Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GD578_RS04820 Genome accession   NZ_CP045560
Coordinates   993240..994277 (-) Length   345 a.a.
NCBI ID   WP_006581387.1    Uniprot ID   A0A0A7XL61
Organism   Acinetobacter nosocomialis strain AC1530     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 988240..999277
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GD578_RS04790 (GD578_04830) - 988525..989457 (-) 933 WP_086610772.1 IS5 family transposase -
  GD578_RS04795 (GD578_04840) - 989631..990698 (+) 1068 WP_004710227.1 hypothetical protein -
  GD578_RS04800 (GD578_04845) - 990698..991021 (+) 324 WP_017393294.1 RnfH family protein -
  GD578_RS04805 (GD578_04850) - 991077..991475 (-) 399 WP_001170994.1 outer membrane protein assembly factor BamE -
  GD578_RS04810 (GD578_04855) fur 991587..992024 (+) 438 WP_002052029.1 ferric iron uptake transcriptional regulator -
  GD578_RS04815 (GD578_04860) pilU 992094..993212 (-) 1119 WP_002052019.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GD578_RS04820 (GD578_04865) pilT 993240..994277 (-) 1038 WP_006581387.1 type IV pilus twitching motility protein PilT Machinery gene
  GD578_RS04825 (GD578_04870) - 994404..995096 (+) 693 WP_017393295.1 YggS family pyridoxal phosphate-dependent enzyme -
  GD578_RS04830 (GD578_04875) - 995204..996046 (+) 843 WP_017393296.1 undecaprenyl-diphosphate phosphatase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38328.92 Da        Isoelectric Point: 6.7271

>NTDB_id=395135 GD578_RS04820 WP_006581387.1 993240..994277(-) (pilT) [Acinetobacter nosocomialis strain AC1530]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMLDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANHGMTTLDQSL
KGLVSRGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=395135 GD578_RS04820 WP_006581387.1 993240..994277(-) (pilT) [Acinetobacter nosocomialis strain AC1530]
ATGGATATTACAGAGCTACTCGCCTTCTCTGTGAAAAATGGCGCATCCGATTTGCACTTATCAGCTGGTATGCCACCAAT
GATTCGGGTCGATGGTGAAGTTCGTCGTATTAACTTGCCAGCCTTAGAACATAAAGATGTCCACCGCCTCGTTTATGACA
TCATGAATGATAAACAGCGCCGTGACTATGAAGAAAAACTCGAAACTGACTTTTCTTTTGAAGTACCGAATGTTGCGCGT
TTCCGTGTGAATGCATTTAACCAAAACCGTGGTGCTGGTGCAGTATTTCGTACCATTCCATCTAAAGTCCTCACCATGGA
AGATCTAGGTCTAGGGCAAATCTTTAAAGATATTTGCGACTATCCTCGTGGCATTGTGCTGGTGACAGGACCAACAGGTT
CGGGTAAATCAACTACGCTTGCAGCGATGCTCGATTATATTAATGAAAACCGCTATGACCATATTTTAACGGTGGAAGAC
CCAATCGAATTTGTACACCAATCTAAGAAATGTCTGATTAACCAACGTGAAGTGCATCGTGATACACATGGCTTTAATGA
AGCCCTTCGCTCAGCACTACGTGAAGACCCAGATATTATTTTAGTCGGTGAGATGCGTGACCTTGAAACGATTCGATTAG
CACTTACTGCTGCCGAAACAGGTCACTTGGTATTTGGTACGCTTCACACCACATCGGCTGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCCGAAGAAAAAGACATGGTTCGTGCCATGCTGTCTGAATCTCTACAAGCCGTTATTTCTCAGAC
CCTGCTTAAAAAGAATGGTGGTGGTCGTGTTGCGGCACATGAAATTATGATTGGTATTCCGGCTATTCGTAACTTAATTC
GTGAAAACAAAGTCGCACAAATGTATTCAGCTATTCAGACTGGTGCTAACCATGGCATGACAACCCTCGACCAAAGCCTA
AAAGGTTTGGTTTCACGTGGTGTAATTAGTCCACAAACAGCCCGTACAGCGGCGAAACAGCCTGAATCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0A7XL61

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

100

100

1
  pilT Acinetobacter baumannii D1279779

99.71

100

0.997
  pilT Acinetobacter baumannii strain A118

99.71

100

0.997
  pilT Acinetobacter baylyi ADP1

88.663

99.71

0.884
  pilT Pseudomonas aeruginosa PAK

84.012

99.71

0.838
  pilT Pseudomonas stutzeri DSM 10701

83.721

99.71

0.835
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.429

97.391

0.696
  pilT Vibrio cholerae strain A1552

71.429

97.391

0.696
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.522
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.101

0.394
  pilU Acinetobacter baylyi ADP1

38.728

100

0.388

Multiple sequence alignment