Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PSESU_RS10380 Genome accession   NC_014924
Coordinates   2280082..2281119 (-) Length   345 a.a.
NCBI ID   WP_013535727.1    Uniprot ID   E6WUB5
Organism   Pseudoxanthomonas suwonensis 11-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2275082..2286119
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSESU_RS10350 (Psesu_2057) ruvX 2275341..2275811 (-) 471 WP_013535721.1 Holliday junction resolvase RuvX -
  PSESU_RS10355 (Psesu_2058) - 2275804..2276370 (-) 567 WP_013535722.1 YqgE/AlgH family protein -
  PSESU_RS10360 (Psesu_2059) - 2276450..2276995 (+) 546 WP_013535723.1 DNA-3-methyladenine glycosylase I -
  PSESU_RS10365 (Psesu_2060) - 2277039..2277908 (+) 870 WP_013535724.1 DUF72 domain-containing protein -
  PSESU_RS10370 (Psesu_2061) - 2277913..2278587 (-) 675 WP_041764836.1 YitT family protein -
  PSESU_RS10375 (Psesu_2062) pilU 2278808..2279938 (-) 1131 WP_013535726.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PSESU_RS10380 (Psesu_2063) pilT 2280082..2281119 (-) 1038 WP_013535727.1 type IV pilus twitching motility protein PilT Machinery gene
  PSESU_RS10385 (Psesu_2064) - 2281235..2281933 (+) 699 WP_049782331.1 YggS family pyridoxal phosphate-dependent enzyme -
  PSESU_RS10390 (Psesu_2065) proC 2281986..2282816 (+) 831 WP_013535729.1 pyrroline-5-carboxylate reductase -
  PSESU_RS10395 (Psesu_2066) - 2282818..2283297 (+) 480 WP_013535730.1 DUF4426 domain-containing protein -
  PSESU_RS10400 (Psesu_2067) - 2283497..2283916 (+) 420 WP_013535731.1 hypothetical protein -
  PSESU_RS10405 (Psesu_2068) - 2283960..2284820 (-) 861 WP_013535732.1 pirin family protein -
  PSESU_RS10410 (Psesu_2069) - 2284945..2285790 (-) 846 WP_013535733.1 ABC transporter permease subunit -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38644.36 Da        Isoelectric Point: 6.7269

>NTDB_id=39493 PSESU_RS10380 WP_013535727.1 2280082..2281119(-) (pilT) [Pseudoxanthomonas suwonensis 11-1]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAAMIDHINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMMTLDQHL
QDLVKRGLVTRQQAKEYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=39493 PSESU_RS10380 WP_013535727.1 2280082..2281119(-) (pilT) [Pseudoxanthomonas suwonensis 11-1]
ATGGATATCGCCGAACTGCTGGCCTTTTCGGTCAAGAACAAGGCCTCGGACCTGCACCTGTCCGCGGGCCTGCCGCCGAT
GATCCGCGTCGATGGCGACGTCCGCCGGATCAACATTCCCGCCCTGGACCACAAGCAGGTCCACGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGCCGGGATTACGAGGAATTCCTCGAGGTCGACTTCTCGTTCGAGATTCCCTCGCTGGCCCGT
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGCGCGGTGTTCCGCACCATTCCGTCCGAGGTCCTCACGCTGGA
GGACCTGGGCTGCCCGCCGATCTTCCGCCAGCTCATCGACCAGCCGCAGGGCCTGATCCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACCACATCAACAAGAACGAGTACGGGCACATCCTCACCGTCGAGGAT
CCGATCGAGTTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGATACCCACGGCTTCAACGA
GGCCCTGCGCTCGGCGCTGCGCGAGGACCCGGACTACATCCTGGTCGGCGAAATGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAGAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCGGTGATCTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGCGGTCGTACCGCGGCCTGGGAAATCATGGTTGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTACTCGTCGATCCAGACCGGCCAGCAGTACGGAATGATGACCCTCGACCAGCACCTG
CAGGACCTGGTCAAGCGCGGCCTGGTCACGCGCCAGCAGGCGAAGGAATACGCCAAGGACAAGCGCCTCTTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E6WUB5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.942

99.71

0.797
  pilT Acinetobacter baumannii D1279779

79.942

99.71

0.797
  pilT Acinetobacter baumannii strain A118

79.942

99.71

0.797
  pilT Pseudomonas stutzeri DSM 10701

79.36

99.71

0.791
  pilT Acinetobacter baylyi ADP1

78.488

99.71

0.783
  pilT Pseudomonas aeruginosa PAK

78.466

98.261

0.771
  pilT Legionella pneumophila strain Lp02

74.419

99.71

0.742
  pilT Legionella pneumophila strain ERS1305867

74.419

99.71

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.149

97.101

0.681
  pilT Vibrio cholerae strain A1552

70.149

97.101

0.681
  pilT Neisseria meningitidis 8013

65.689

98.841

0.649
  pilT Neisseria gonorrhoeae MS11

65.396

98.841

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.145

100

0.501
  pilU Vibrio cholerae strain A1552

39.943

100

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397
  pilU Acinetobacter baylyi ADP1

40

98.551

0.394

Multiple sequence alignment