Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PSESU_RS10375 Genome accession   NC_014924
Coordinates   2278808..2279938 (-) Length   376 a.a.
NCBI ID   WP_013535726.1    Uniprot ID   E6WUB4
Organism   Pseudoxanthomonas suwonensis 11-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2273808..2284938
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSESU_RS10345 (Psesu_2056) - 2274338..2275282 (-) 945 WP_013535720.1 aspartate carbamoyltransferase catalytic subunit -
  PSESU_RS10350 (Psesu_2057) ruvX 2275341..2275811 (-) 471 WP_013535721.1 Holliday junction resolvase RuvX -
  PSESU_RS10355 (Psesu_2058) - 2275804..2276370 (-) 567 WP_013535722.1 YqgE/AlgH family protein -
  PSESU_RS10360 (Psesu_2059) - 2276450..2276995 (+) 546 WP_013535723.1 DNA-3-methyladenine glycosylase I -
  PSESU_RS10365 (Psesu_2060) - 2277039..2277908 (+) 870 WP_013535724.1 DUF72 domain-containing protein -
  PSESU_RS10370 (Psesu_2061) - 2277913..2278587 (-) 675 WP_041764836.1 YitT family protein -
  PSESU_RS10375 (Psesu_2062) pilU 2278808..2279938 (-) 1131 WP_013535726.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PSESU_RS10380 (Psesu_2063) pilT 2280082..2281119 (-) 1038 WP_013535727.1 type IV pilus twitching motility protein PilT Machinery gene
  PSESU_RS10385 (Psesu_2064) - 2281235..2281933 (+) 699 WP_049782331.1 YggS family pyridoxal phosphate-dependent enzyme -
  PSESU_RS10390 (Psesu_2065) proC 2281986..2282816 (+) 831 WP_013535729.1 pyrroline-5-carboxylate reductase -
  PSESU_RS10395 (Psesu_2066) - 2282818..2283297 (+) 480 WP_013535730.1 DUF4426 domain-containing protein -
  PSESU_RS10400 (Psesu_2067) - 2283497..2283916 (+) 420 WP_013535731.1 hypothetical protein -
  PSESU_RS10405 (Psesu_2068) - 2283960..2284820 (-) 861 WP_013535732.1 pirin family protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41925.30 Da        Isoelectric Point: 6.8287

>NTDB_id=39492 PSESU_RS10375 WP_013535726.1 2278808..2279938(-) (pilU) [Pseudoxanthomonas suwonensis 11-1]
MSTMDFTSFLKLMAHQKASDLFITAGMPPAMKVHGKITPITQTPLTPQQSRDMVLNVMTPAQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELNLPPIIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIVTQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRSQLLMDLSLNLKGVVAQQLVPTPDGKGRRVAMEILLGTPLVQDYIRDGEIHKLKEVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=39492 PSESU_RS10375 WP_013535726.1 2278808..2279938(-) (pilU) [Pseudoxanthomonas suwonensis 11-1]
ATGAGCACCATGGATTTCACCTCGTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGACCTGTTCATCACCGCGGGCAT
GCCGCCGGCGATGAAGGTCCACGGCAAGATCACGCCGATCACGCAGACGCCGCTGACCCCGCAGCAGAGCCGCGACATGG
TCCTCAACGTGATGACCCCGGCCCAGCGCGAGGAGTTCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCGTCGCCGGC
GTGGGCCGCTTCCGCGTCAGCTGTTTCTACCAGCGCAACCAGGTGGGCATGGTGCTGCGCCGGATCGAGACCAGGATCCC
GACGGTGGAGGAGCTGAACCTGCCGCCGATCATCAAGACCCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAACTCGACCGGCCACATCATCACC
ATCGAGGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCGTCACCCAGCGCGAGGTCGGCATCGACACCGACAG
CTGGGAGGCCGCGCTGAAGAACACCCTGCGCCAGGCCCCGGACGTGATCATGATCGGCGAAGTGCGTACCCGCGAGGGCA
TGGACCACGCCATCGCCTTCGCCGAGACCGGCCACCTCGTGCTGTGCACCCTGCATGCGAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAGGATCGCCGCAGCCAGCTGCTGATGGACCTGTCGCTGAACCTCAAGGGCGTGGT
CGCGCAGCAGCTGGTGCCGACCCCCGACGGCAAGGGGCGCCGCGTGGCGATGGAGATCCTGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGGTCATGAAGGAATCGACCAACCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCTCCTACGAGGACGCGCTGCGCTACGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTCTCCCAGGGCGGCGACGCCCGCACCCTGTCGCAGGGGCTGGACGGGGTCGAGATCGCCG
AGGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E6WUB4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

69.21

97.606

0.676
  pilU Acinetobacter baylyi ADP1

64.426

94.947

0.612
  pilU Vibrio cholerae strain A1552

54.494

94.681

0.516
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.737

94.947

0.396
  pilT Legionella pneumophila strain Lp02

41.493

89.096

0.37
  pilT Legionella pneumophila strain ERS1305867

41.493

89.096

0.37
  pilT Acinetobacter baumannii strain A118

40.116

91.489

0.367
  pilT Acinetobacter nosocomialis M2

40.116

91.489

0.367
  pilT Acinetobacter baumannii D1279779

40.116

91.489

0.367
  pilT Pseudomonas aeruginosa PAK

40.597

89.096

0.362

Multiple sequence alignment