Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FS418_RS07935 Genome accession   NZ_CP045427
Coordinates   1883657..1884694 (+) Length   345 a.a.
NCBI ID   WP_142872474.1    Uniprot ID   -
Organism   Shewanella sp. YLB-09     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1878657..1889694
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FS418_RS07905 (FS418_07950) rdgB 1879685..1880287 (-) 603 WP_142872469.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  FS418_RS07910 (FS418_07955) - 1880453..1880887 (-) 435 WP_142872470.1 DUF4426 domain-containing protein -
  FS418_RS07915 (FS418_07960) yggU 1881039..1881341 (-) 303 WP_185965788.1 DUF167 family protein YggU -
  FS418_RS07920 (FS418_07965) - 1881348..1881896 (-) 549 WP_142872471.1 YggT family protein -
  FS418_RS07925 (FS418_07970) proC 1881967..1882785 (-) 819 WP_142872472.1 pyrroline-5-carboxylate reductase -
  FS418_RS07930 (FS418_07975) - 1882925..1883620 (-) 696 WP_142872473.1 YggS family pyridoxal phosphate-dependent enzyme -
  FS418_RS07935 (FS418_07980) pilT 1883657..1884694 (+) 1038 WP_142872474.1 type IV pilus twitching motility protein PilT Machinery gene
  FS418_RS07940 (FS418_07985) pilU 1884718..1885830 (+) 1113 WP_142872475.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FS418_RS07945 (FS418_07990) - 1885998..1887101 (+) 1104 WP_142872476.1 hypothetical protein -
  FS418_RS07950 (FS418_07995) ruvX 1887530..1887952 (-) 423 WP_142872477.1 Holliday junction resolvase RuvX -
  FS418_RS07955 (FS418_08000) - 1888055..1888615 (-) 561 WP_142872478.1 YqgE/AlgH family protein -
  FS418_RS07960 (FS418_08005) yciH 1888680..1889009 (-) 330 WP_142872479.1 stress response translation initiation inhibitor YciH -
  FS418_RS07965 (FS418_08010) - 1889168..1889485 (-) 318 WP_142872480.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38306.81 Da        Isoelectric Point: 6.9449

>NTDB_id=394034 FS418_RS07935 WP_142872474.1 1883657..1884694(+) (pilT) [Shewanella sp. YLB-09]
MEITELLAFSVKHKASDLHLSAGVSPMIRVDGDVRKINLPALDHQGVHGLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQARGAAAVFRTIPSDILSLEQLGAPEIFKKISSFPRGLVLVTGPTGSGKSTTLAAMVDYINESRHEHILTIED
PIEFVHQSKQCLVNQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPEGEKGMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMSHGMQTLDQCL
QNLVNRGQISREDAQHKSANKQTQF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=394034 FS418_RS07935 WP_142872474.1 1883657..1884694(+) (pilT) [Shewanella sp. YLB-09]
ATGGAAATCACAGAGTTACTTGCTTTTAGTGTAAAACACAAAGCGTCAGATCTACACCTTTCAGCAGGCGTTTCGCCTAT
GATACGTGTAGATGGCGATGTCAGAAAGATCAATTTACCCGCTTTAGATCATCAAGGGGTGCATGGACTCGTCTATGACA
TCATGAATGACAAGCAGCGTAAAGATTATGAAGAACATTTAGAGATAGATTTTTCCTTCGAAGTCCCTAATCTAGCGCGC
TTTCGTGTCAATGCCTTCAATCAGGCGCGAGGAGCTGCGGCAGTATTTCGAACCATTCCCAGTGATATCTTGAGCCTAGA
GCAACTAGGTGCTCCGGAGATTTTTAAGAAAATTTCCAGCTTCCCGCGTGGCCTTGTCCTTGTTACTGGTCCGACTGGCT
CGGGTAAGAGTACCACTTTAGCGGCGATGGTCGATTATATTAATGAAAGTCGCCATGAGCATATCCTTACCATTGAGGAC
CCAATAGAATTTGTCCACCAGAGTAAACAGTGTCTGGTTAACCAGCGTGAAGTGCACCGTCATACTCACAGCTTTAATGC
TGCACTGAGAAGTGCACTGCGTGAAGATCCAGATGTGATTTTGGTCGGTGAGATGCGTGACCTAGAGACAATACGTTTAG
CGATGACGGCGGCTGAAACTGGTCACTTAGTTTTCGGTACACTGCACACCACCTCAGCGGCTAAAACCATCGACCGTGTG
GTTGATGTATTCCCTGAAGGTGAGAAGGGCATGGTCAGAACCATGCTGTCAGAATCTCTGCAGGCGGTTATTTCACAAAC
CTTGATAAAGAAAGTGGGTGGCGGACGAGTTGCGGCGCATGAGATCATGATGGGAACACCTGCCATTCGAAACTTAATTC
GTGAGGATAAAGTGGCGCAGATGTATTCAGCGATTCAGACGGGGATGTCACATGGTATGCAGACGTTAGATCAATGCCTG
CAAAACTTGGTGAATCGCGGTCAGATAAGTCGTGAAGATGCGCAGCACAAGAGTGCAAATAAGCAGACACAATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Pseudomonas aeruginosa PAK

80.178

97.971

0.786
  pilT Pseudomonas stutzeri DSM 10701

79.586

97.971

0.78
  pilT Acinetobacter baylyi ADP1

78.209

97.101

0.759
  pilT Legionella pneumophila strain Lp02

76.246

98.841

0.754
  pilT Legionella pneumophila strain ERS1305867

76.246

98.841

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.81

97.681

0.701
  pilT Vibrio cholerae strain A1552

71.81

97.681

0.701
  pilT Neisseria meningitidis 8013

69.186

99.71

0.69
  pilT Neisseria gonorrhoeae MS11

68.895

99.71

0.687
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.765

94.783

0.481
  pilU Vibrio cholerae strain A1552

41.983

99.42

0.417
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.101

0.403
  pilU Acinetobacter baylyi ADP1

39.583

97.391

0.386

Multiple sequence alignment