Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FS418_RS07940 Genome accession   NZ_CP045427
Coordinates   1884718..1885830 (+) Length   370 a.a.
NCBI ID   WP_142872475.1    Uniprot ID   A0AAJ4APZ3
Organism   Shewanella sp. YLB-09     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1879718..1890830
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FS418_RS07910 (FS418_07955) - 1880453..1880887 (-) 435 WP_142872470.1 DUF4426 domain-containing protein -
  FS418_RS07915 (FS418_07960) yggU 1881039..1881341 (-) 303 WP_185965788.1 DUF167 family protein YggU -
  FS418_RS07920 (FS418_07965) - 1881348..1881896 (-) 549 WP_142872471.1 YggT family protein -
  FS418_RS07925 (FS418_07970) proC 1881967..1882785 (-) 819 WP_142872472.1 pyrroline-5-carboxylate reductase -
  FS418_RS07930 (FS418_07975) - 1882925..1883620 (-) 696 WP_142872473.1 YggS family pyridoxal phosphate-dependent enzyme -
  FS418_RS07935 (FS418_07980) pilT 1883657..1884694 (+) 1038 WP_142872474.1 type IV pilus twitching motility protein PilT Machinery gene
  FS418_RS07940 (FS418_07985) pilU 1884718..1885830 (+) 1113 WP_142872475.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FS418_RS07945 (FS418_07990) - 1885998..1887101 (+) 1104 WP_142872476.1 hypothetical protein -
  FS418_RS07950 (FS418_07995) ruvX 1887530..1887952 (-) 423 WP_142872477.1 Holliday junction resolvase RuvX -
  FS418_RS07955 (FS418_08000) - 1888055..1888615 (-) 561 WP_142872478.1 YqgE/AlgH family protein -
  FS418_RS07960 (FS418_08005) yciH 1888680..1889009 (-) 330 WP_142872479.1 stress response translation initiation inhibitor YciH -
  FS418_RS07965 (FS418_08010) - 1889168..1889485 (-) 318 WP_142872480.1 hypothetical protein -
  FS418_RS07970 (FS418_08015) - 1889681..1890241 (+) 561 WP_142872481.1 DUF4136 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41014.78 Da        Isoelectric Point: 5.4391

>NTDB_id=394035 FS418_RS07940 WP_142872475.1 1884718..1885830(+) (pilU) [Shewanella sp. YLB-09]
MDVRPFLKNMVDRKASDLFITAGFPPSAKIDGELRTLSETSFSPAQSLDFVESLMSDEQKKEFHETRECNFAFALNDLGR
FRVSAFWQREASGCVMRRIETTIPDVDDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLASLVGYRNANSRGHILTIED
PVEFVHDHRKSIVTQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALAFAETGHLCMATLHANNANQALDRI
MNLVPVSKHQQLLFDLSLNLRGIVAQQLVPTADGKGRRAAIEILINTPRVSSLIAKNELHSLKETMAKSNEQGMQTFDQA
LLNLYVEGEISYADALHHADSPNDLRLMIKLQNSEQTGSGFMEGVTLDLD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=394035 FS418_RS07940 WP_142872475.1 1884718..1885830(+) (pilU) [Shewanella sp. YLB-09]
ATGGATGTTCGTCCCTTTTTAAAAAACATGGTAGATCGTAAAGCATCAGATCTATTTATTACAGCCGGTTTTCCACCGAG
TGCCAAAATTGATGGTGAGCTAAGAACTTTGAGTGAAACTTCATTTTCTCCGGCTCAGTCGCTCGATTTTGTCGAGTCTT
TGATGAGCGATGAACAGAAAAAGGAGTTCCATGAGACTCGTGAATGTAACTTTGCATTCGCGCTCAATGATCTAGGCCGT
TTTCGTGTCAGTGCATTCTGGCAGCGTGAGGCGTCAGGCTGTGTAATGCGCCGGATTGAGACTACAATTCCCGATGTCGA
TGACCTGAAGCTTCCTCCAATCCTTAAAGACTTGGTGATGAGTAAACGTGGTTTGATCATCATGGTTGGGGGAACTGGTA
CGGGTAAGTCTACCTCTTTGGCTTCCTTGGTGGGCTATAGAAATGCTAATTCCCGTGGTCACATTCTGACGATAGAGGAC
CCAGTCGAATTTGTGCATGATCACCGTAAAAGTATTGTCACACAGCGTGAAGTGGGGATTGATACTGAGTCTTTCGATGC
TGCACTTAAGAGTTCACTGCGTCAAGCACCAGATGTGATCTTAATTGGTGAGATCCGTACTCAGGAAACCATGGAGTTTG
CCTTGGCTTTCGCCGAAACGGGGCATCTTTGTATGGCAACCTTACACGCCAATAACGCTAACCAAGCGTTAGATCGGATC
ATGAATTTGGTTCCTGTCAGTAAGCATCAGCAGTTATTGTTTGACCTATCGCTGAACTTAAGAGGCATTGTTGCTCAGCA
ACTTGTGCCAACGGCTGATGGCAAGGGACGACGCGCAGCTATTGAGATCCTGATCAATACGCCGCGGGTAAGTAGCTTGA
TTGCAAAAAATGAGCTGCATTCACTTAAAGAAACCATGGCTAAGTCTAATGAGCAGGGAATGCAAACTTTCGATCAGGCT
TTACTTAATCTCTACGTCGAAGGTGAGATTAGTTATGCTGATGCCTTGCACCACGCCGATTCCCCTAACGATTTAAGATT
GATGATTAAGCTGCAGAACTCAGAGCAAACGGGGTCTGGCTTTATGGAAGGGGTGACGCTAGATTTAGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.559

100

0.619
  pilU Acinetobacter baylyi ADP1

60.795

95.135

0.578
  pilU Vibrio cholerae strain A1552

57.568

100

0.576
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.667

97.297

0.405
  pilT Vibrio cholerae strain A1552

41.818

89.189

0.373
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.818

89.189

0.373
  pilT Legionella pneumophila strain Lp02

41.39

89.459

0.37
  pilT Legionella pneumophila strain ERS1305867

41.39

89.459

0.37

Multiple sequence alignment