Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FIU82_RS03240 Genome accession   NZ_CP045418
Coordinates   703626..704675 (+) Length   349 a.a.
NCBI ID   WP_022946317.1    Uniprot ID   A0A0F4PM94
Organism   Pseudoalteromonas sp. THAF3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 698626..709675
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIU82_RS03205 (FIU82_03230) - 698639..699157 (-) 519 WP_022946324.1 DsrE family protein -
  FIU82_RS03210 (FIU82_03235) hemW 699202..700347 (-) 1146 WP_152440325.1 radical SAM family heme chaperone HemW -
  FIU82_RS03215 (FIU82_03240) - 700351..700935 (-) 585 WP_152440326.1 XTP/dITP diphosphatase -
  FIU82_RS03220 (FIU82_03245) - 700946..701383 (-) 438 WP_053910609.1 DUF4426 domain-containing protein -
  FIU82_RS03225 (FIU82_03250) - 701488..702021 (-) 534 WP_022946320.1 YggT family protein -
  FIU82_RS03230 (FIU82_03255) proC 702042..702863 (-) 822 WP_152440327.1 pyrroline-5-carboxylate reductase -
  FIU82_RS03235 (FIU82_03260) - 702909..703595 (-) 687 WP_152440328.1 YggS family pyridoxal phosphate-dependent enzyme -
  FIU82_RS03240 (FIU82_03265) pilT 703626..704675 (+) 1050 WP_022946317.1 type IV pilus twitching motility protein PilT Machinery gene
  FIU82_RS03245 (FIU82_03270) pilU 704689..705822 (+) 1134 WP_022946316.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FIU82_RS03250 (FIU82_03275) - 705800..706723 (+) 924 WP_253946338.1 hypothetical protein -
  FIU82_RS03255 (FIU82_03280) ruvX 706683..707132 (-) 450 WP_022946314.1 Holliday junction resolvase RuvX -
  FIU82_RS03260 (FIU82_03285) - 707129..707686 (-) 558 WP_022946313.1 YqgE/AlgH family protein -
  FIU82_RS03265 (FIU82_03290) gshB 707701..708654 (-) 954 WP_045979570.1 glutathione synthase -
  FIU82_RS03270 (FIU82_03295) rsmE 708709..709443 (-) 735 WP_026111304.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38685.18 Da        Isoelectric Point: 6.2340

>NTDB_id=393897 FIU82_RS03240 WP_022946317.1 703626..704675(+) (pilT) [Pseudoalteromonas sp. THAF3]
MDITELLAFSVQHKASDLHLSAGVSPMIRVDGDVRRINIPVLEAKEVSGLVYDIMNDKQRKEYEENLEVDFSFEVPDLAR
FRVNAFNSNRGPAAVFRTIPNEVLTLDDLGAPEIFKTISENPRGLVLVTGPTGSGKSTTLAAMIDYINKSRHDHILTIED
PIEFVHQNKLSLINQREVHRDTHSFNNALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPGEEKDMVRSMLSESLRAVISQTLLKKVSGGRIAAHEIMIGIPAIRNLIREDKIAQMYSSIQTGAAHGMQTMDQCL
QNLLNRGLISTQAAQAKAHDKTQFGMSGQ

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=393897 FIU82_RS03240 WP_022946317.1 703626..704675(+) (pilT) [Pseudoalteromonas sp. THAF3]
ATGGATATTACTGAATTATTGGCCTTTAGTGTGCAACACAAGGCCTCGGATTTACACCTTTCAGCCGGTGTATCGCCGAT
GATTCGTGTCGACGGCGACGTGCGCCGTATTAACATTCCCGTGCTTGAGGCCAAGGAAGTCAGCGGCCTTGTCTACGACA
TTATGAACGATAAACAGCGCAAAGAATACGAAGAAAACCTAGAAGTGGATTTCTCGTTCGAGGTTCCTGACTTAGCGCGT
TTTCGTGTTAACGCCTTTAATTCTAACCGAGGCCCAGCAGCGGTGTTTCGTACCATCCCCAATGAGGTGCTGACCTTGGA
TGATTTGGGCGCGCCGGAGATATTTAAAACCATCTCGGAAAACCCACGTGGTCTGGTATTGGTGACTGGGCCAACAGGTT
CTGGTAAATCAACCACCTTGGCGGCGATGATTGATTACATCAATAAATCACGCCACGACCATATCCTCACCATTGAGGAC
CCGATAGAATTCGTACACCAAAACAAGCTAAGCTTGATTAACCAGCGGGAAGTTCACCGTGATACGCACAGCTTTAACAA
TGCCTTGCGCAGTGCGTTGCGTGAAGACCCCGACGTTATCTTGGTCGGTGAGCTGCGGGACTTAGAAACCATTCGCCTAG
CGATGACCGCAGCAGAAACAGGTCACTTAGTGTTTGGCACCTTGCACACCACCTCAGCACCAAAAACCATTGACCGTATT
ATTGACGTCTTCCCCGGTGAAGAAAAAGACATGGTACGTTCGATGCTTTCAGAATCACTGCGCGCGGTTATCTCGCAAAC
ACTGTTGAAAAAAGTGTCGGGCGGGCGAATTGCGGCGCACGAGATTATGATAGGTATCCCAGCGATACGAAACCTTATTC
GTGAAGATAAAATCGCGCAGATGTACTCGTCGATTCAAACTGGCGCTGCGCATGGTATGCAGACCATGGACCAGTGCTTA
CAAAACTTGTTAAACCGTGGCCTTATCAGCACACAGGCAGCGCAGGCGAAAGCCCATGATAAGACCCAATTCGGCATGTC
GGGTCAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F4PM94

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.907

98.567

0.768
  pilT Acinetobacter baumannii D1279779

77.907

98.567

0.768
  pilT Acinetobacter baumannii strain A118

77.907

98.567

0.768
  pilT Acinetobacter baylyi ADP1

77.035

98.567

0.759
  pilT Pseudomonas stutzeri DSM 10701

75.291

98.567

0.742
  pilT Pseudomonas aeruginosa PAK

74.709

98.567

0.736
  pilT Legionella pneumophila strain Lp02

73.837

98.567

0.728
  pilT Legionella pneumophila strain ERS1305867

73.837

98.567

0.728
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.107

96.562

0.696
  pilT Vibrio cholerae strain A1552

72.107

96.562

0.696
  pilT Neisseria gonorrhoeae MS11

70.674

97.708

0.691
  pilT Neisseria meningitidis 8013

70.674

97.708

0.691
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

98.854

0.521
  pilU Vibrio cholerae strain A1552

41.493

95.989

0.398
  pilU Pseudomonas stutzeri DSM 10701

40.896

95.989

0.393
  pilU Acinetobacter baylyi ADP1

40.663

95.129

0.387

Multiple sequence alignment