Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FIU82_RS03245 Genome accession   NZ_CP045418
Coordinates   704689..705822 (+) Length   377 a.a.
NCBI ID   WP_022946316.1    Uniprot ID   A0A0F4PSH8
Organism   Pseudoalteromonas sp. THAF3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 699689..710822
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIU82_RS03215 (FIU82_03240) - 700351..700935 (-) 585 WP_152440326.1 XTP/dITP diphosphatase -
  FIU82_RS03220 (FIU82_03245) - 700946..701383 (-) 438 WP_053910609.1 DUF4426 domain-containing protein -
  FIU82_RS03225 (FIU82_03250) - 701488..702021 (-) 534 WP_022946320.1 YggT family protein -
  FIU82_RS03230 (FIU82_03255) proC 702042..702863 (-) 822 WP_152440327.1 pyrroline-5-carboxylate reductase -
  FIU82_RS03235 (FIU82_03260) - 702909..703595 (-) 687 WP_152440328.1 YggS family pyridoxal phosphate-dependent enzyme -
  FIU82_RS03240 (FIU82_03265) pilT 703626..704675 (+) 1050 WP_022946317.1 type IV pilus twitching motility protein PilT Machinery gene
  FIU82_RS03245 (FIU82_03270) pilU 704689..705822 (+) 1134 WP_022946316.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FIU82_RS03250 (FIU82_03275) - 705800..706723 (+) 924 WP_253946338.1 hypothetical protein -
  FIU82_RS03255 (FIU82_03280) ruvX 706683..707132 (-) 450 WP_022946314.1 Holliday junction resolvase RuvX -
  FIU82_RS03260 (FIU82_03285) - 707129..707686 (-) 558 WP_022946313.1 YqgE/AlgH family protein -
  FIU82_RS03265 (FIU82_03290) gshB 707701..708654 (-) 954 WP_045979570.1 glutathione synthase -
  FIU82_RS03270 (FIU82_03295) rsmE 708709..709443 (-) 735 WP_026111304.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  FIU82_RS03275 (FIU82_03300) - 709481..709909 (-) 429 WP_152440329.1 hypothetical protein -
  FIU82_RS03280 (FIU82_03305) - 709928..710818 (-) 891 WP_022946309.1 hypothetical protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41672.63 Da        Isoelectric Point: 5.4104

>NTDB_id=393898 FIU82_RS03245 WP_022946316.1 704689..705822(+) (pilU) [Pseudoalteromonas sp. THAF3]
MIQPLAHYLQKMIALQASDLFVSTHLPVSAKVNGELTPLDDYVLSDDEALTLVESAMSDKQINEFHNSKECNFAIATDEG
RFRISAFWQRDQAGMVIRRIVTTIPDVNELGLPSVLTDVIMKKRGLVLFVGGTGTGKSTSLAALLGYRNRNQKGHILTIE
DPIEFVHEHNKSIITQREVGLDTESFEAALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDR
IMHLVPKEKHDKLKYDLALNLRAIIAQQLIPTTDGNGRVAAIEVLLNSPMIAELIKKGEIGSIKEVMAKSKEMGMQTFDQ
ALFDLYKQNLINYADALHHADSPNDLRLMIKLRNNEQQGAGFLQGVTVDGLGDDSDS

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=393898 FIU82_RS03245 WP_022946316.1 704689..705822(+) (pilU) [Pseudoalteromonas sp. THAF3]
ATGATACAACCTTTAGCGCATTATTTGCAGAAGATGATCGCCTTGCAGGCATCGGATTTATTTGTTTCTACGCATTTGCC
GGTGAGTGCCAAAGTGAATGGCGAGCTCACCCCCCTTGATGACTATGTGCTCAGTGATGATGAGGCATTGACCTTGGTAG
AATCGGCAATGTCCGATAAACAAATCAATGAGTTTCATAACTCTAAAGAATGTAACTTTGCCATTGCCACCGATGAGGGA
CGTTTCCGTATCTCGGCATTTTGGCAACGTGATCAGGCGGGGATGGTTATTCGTCGTATCGTTACCACCATACCCGACGT
TAATGAACTCGGTCTGCCTTCAGTGCTTACCGATGTGATCATGAAAAAACGTGGTCTAGTGCTGTTCGTCGGTGGTACCG
GCACCGGTAAGTCGACCTCATTGGCGGCGCTACTGGGTTATCGTAACCGCAACCAAAAAGGCCATATCCTTACCATTGAG
GATCCAATTGAATTCGTACACGAGCATAACAAAAGTATCATTACCCAACGTGAGGTTGGACTTGATACCGAGAGTTTTGA
GGCTGCACTGAAGAGCTCGCTGCGTCAGGCGCCGGATGTAATTCTCATTGGTGAGATCCGCTCGCAAGAAACCATGGAAT
ATGCGTTAAGCTTCGCTGAAACTGGCCATCTGTGTGTTGCTACCTTGCACGCCAACAACGCCAACCAAGCCATTGACCGT
ATTATGCACTTGGTGCCCAAGGAAAAGCATGACAAGCTCAAATATGATTTAGCCCTTAACTTGCGCGCCATTATTGCTCA
GCAGCTGATCCCTACCACAGATGGTAATGGTCGTGTGGCAGCGATTGAGGTGTTACTAAACTCACCGATGATTGCCGAGC
TGATCAAAAAAGGCGAAATCGGCTCCATCAAAGAGGTGATGGCTAAGTCCAAAGAAATGGGCATGCAAACCTTCGACCAA
GCGCTGTTTGATCTGTACAAGCAAAACCTTATCAACTACGCCGATGCGCTGCACCACGCTGACTCACCGAACGATCTTCG
TTTGATGATTAAGCTTCGTAACAACGAACAACAAGGTGCAGGCTTCCTACAAGGGGTAACGGTGGACGGCCTTGGCGACG
ATAGCGATAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F4PSH8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.297

98.143

0.562
  pilU Acinetobacter baylyi ADP1

56.873

98.408

0.56
  pilU Vibrio cholerae strain A1552

57.534

96.817

0.557
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.642

91.777

0.401
  pilT Acinetobacter baylyi ADP1

41.337

87.268

0.361

Multiple sequence alignment