Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FIU83_RS16490 Genome accession   NZ_CP045417
Coordinates   3622353..3623384 (-) Length   343 a.a.
NCBI ID   WP_152485024.1    Uniprot ID   A0A5P9IZU8
Organism   Halomonas sp. THAF5a     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3617353..3628384
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIU83_RS16475 (FIU83_16485) tyrS 3618720..3619919 (-) 1200 WP_152485021.1 tyrosine--tRNA ligase -
  FIU83_RS16480 (FIU83_16490) - 3620033..3621163 (+) 1131 WP_152485022.1 anhydro-N-acetylmuramic acid kinase -
  FIU83_RS16485 (FIU83_16495) pilU 3621185..3622327 (-) 1143 WP_152485023.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FIU83_RS16490 (FIU83_16500) pilT 3622353..3623384 (-) 1032 WP_152485024.1 type IV pilus twitching motility protein PilT Machinery gene
  FIU83_RS16495 (FIU83_16505) - 3623448..3624152 (+) 705 WP_152485025.1 YggS family pyridoxal phosphate-dependent enzyme -
  FIU83_RS16500 (FIU83_16510) proC 3624208..3625032 (+) 825 WP_152485026.1 pyrroline-5-carboxylate reductase -
  FIU83_RS16505 (FIU83_16515) - 3625087..3625680 (+) 594 WP_152485027.1 YggT family protein -
  FIU83_RS16510 (FIU83_16520) - 3625721..3626878 (+) 1158 WP_152485028.1 homoserine O-acetyltransferase -
  FIU83_RS16515 (FIU83_16525) metW 3626878..3627474 (+) 597 WP_152485414.1 methionine biosynthesis protein MetW -
  FIU83_RS16520 (FIU83_16530) - 3627551..3628003 (+) 453 WP_152485029.1 DUF4426 domain-containing protein -
  FIU83_RS16525 (FIU83_16535) - 3628000..3628329 (+) 330 WP_152485030.1 Rieske (2Fe-2S) protein -

Sequence

Protein


Download         Length: 343 a.a.        Molecular weight: 37943.48 Da        Isoelectric Point: 6.3154

>NTDB_id=393889 FIU83_RS16490 WP_152485024.1 3622353..3623384(-) (pilT) [Halomonas sp. THAF5a]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDIRRLNVPSMEDREVRKLIYDIMADRQRRDYEEFYETDFSFEVPGVSR
FRVNVFNQARGAGAVFRTIPTEVLSMEDLGMGTVFRQLSMLPRGLVLVTGPTGSGKSTTLAAMIDYINDNRFEHILTIED
PVEFVHRSKRCLVNQREVHRDTHGFAPALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPGEEKSMVRSMLSESLQAVISQTLLKRQGGGRVAAHEILVATAAVRNLIREDKVAQIYSAIQTGGNLGMQTMDAAL
ARLVSDNIVAREEAQAKAKGVLA

Nucleotide


Download         Length: 1032 bp        

>NTDB_id=393889 FIU83_RS16490 WP_152485024.1 3622353..3623384(-) (pilT) [Halomonas sp. THAF5a]
ATGGATATTACCGAACTGCTGGCATTCTCGGCAAAACAGAACGCCTCGGATCTTCACCTCTCGGCGGGCCTGCCGCCGAT
GATCCGCGTCGATGGCGACATCCGACGACTCAACGTGCCGTCCATGGAGGACCGAGAGGTCCGCAAGCTGATCTACGACA
TCATGGCCGACCGCCAGCGTCGCGACTACGAGGAGTTCTACGAGACCGACTTCTCGTTCGAGGTGCCGGGGGTCTCCCGC
TTCCGCGTCAACGTCTTCAACCAGGCGCGCGGTGCCGGGGCGGTGTTCCGCACCATCCCCACCGAGGTACTGTCCATGGA
GGACCTCGGCATGGGGACGGTCTTCCGCCAGCTCTCGATGCTGCCCCGCGGCCTGGTGCTGGTCACCGGCCCCACCGGCT
CGGGCAAGAGCACCACCCTGGCGGCGATGATCGACTACATCAACGACAACCGCTTCGAGCACATCCTCACCATCGAGGAC
CCGGTGGAATTCGTCCACCGCAGCAAGCGCTGCCTGGTCAACCAGCGCGAGGTGCACCGCGATACCCACGGCTTCGCCCC
GGCCCTGCGCAGCGCCCTGCGCGAGGACCCGGACATCATCCTGGTCGGCGAGCTGCGCGACCTCGAGACCATCCGGCTGG
CGCTGACCGCGGCCGAGACGGGCCACCTGGTGTTCGGCACCCTGCACACCACCTCGGCGGCCAAGACCATCGACCGGATC
ATCGACGTCTTCCCGGGCGAGGAGAAGTCCATGGTGCGCTCGATGCTCTCCGAATCGCTGCAGGCGGTGATCTCCCAGAC
CCTGCTCAAGCGCCAGGGCGGCGGCCGGGTGGCGGCCCACGAGATCCTGGTGGCCACGGCGGCGGTGCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATCTACTCGGCGATCCAGACCGGCGGCAACCTCGGCATGCAGACCATGGATGCCGCCCTG
GCGCGGCTGGTCAGCGACAACATCGTGGCGCGGGAGGAGGCCCAGGCCAAGGCCAAGGGCGTGCTGGCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5P9IZU8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

78.761

98.834

0.778
  pilT Pseudomonas aeruginosa PAK

78.761

98.834

0.778
  pilT Acinetobacter nosocomialis M2

75.811

98.834

0.749
  pilT Acinetobacter baumannii D1279779

75.516

98.834

0.746
  pilT Acinetobacter baumannii strain A118

75.516

98.834

0.746
  pilT Acinetobacter baylyi ADP1

74.926

98.834

0.741
  pilT Legionella pneumophila strain Lp02

72.189

98.542

0.711
  pilT Legionella pneumophila strain ERS1305867

72.189

98.542

0.711
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.953

98.251

0.668
  pilT Vibrio cholerae strain A1552

67.953

98.251

0.668
  pilT Neisseria meningitidis 8013

65.588

99.125

0.65
  pilT Neisseria gonorrhoeae MS11

65.294

99.125

0.647
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.212

98.834

0.516
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.668

0.402
  pilU Vibrio cholerae strain A1552

40

97.668

0.391
  pilU Acinetobacter baylyi ADP1

39.118

99.125

0.388

Multiple sequence alignment