Detailed information NTG0393888

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: FIU83_RS16485
• Genome accession: NZ_CP045417
• Coordinates: 3621185..3622327 (-)
• Length: 380 a.a.
• NCBI ID: WP_152485023.1
• Uniprot ID: A0A5P9IT93
• Organism: Halomonas sp. THAF5a
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3616185..3627327

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FIU83_RS16475 (FIU83_16485)	tyrS	3618720..3619919 (-)	1200	WP_152485021.1	tyrosine--tRNA ligase	-
FIU83_RS16480 (FIU83_16490)	-	3620033..3621163 (+)	1131	WP_152485022.1	anhydro-N-acetylmuramic acid kinase	-
FIU83_RS16485 (FIU83_16495)	pilU	3621185..3622327 (-)	1143	WP_152485023.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
FIU83_RS16490 (FIU83_16500)	pilT	3622353..3623384 (-)	1032	WP_152485024.1	type IV pilus twitching motility protein PilT	Machinery gene
FIU83_RS16495 (FIU83_16505)	-	3623448..3624152 (+)	705	WP_152485025.1	YggS family pyridoxal phosphate-dependent enzyme	-
FIU83_RS16500 (FIU83_16510)	proC	3624208..3625032 (+)	825	WP_152485026.1	pyrroline-5-carboxylate reductase	-
FIU83_RS16505 (FIU83_16515)	-	3625087..3625680 (+)	594	WP_152485027.1	YggT family protein	-
FIU83_RS16510 (FIU83_16520)	-	3625721..3626878 (+)	1158	WP_152485028.1	homoserine O-acetyltransferase	-

Sequence

Protein

• Length: 380 a.a.
• Molecular weight: 42048.34 Da
• Isoelectric Point: 6.2374

>NTDB_id=393888 FIU83_RS16485 WP_152485023.1 3621185..3622327(-) (pilU) [Halomonas sp. THAF5a]
MTAKEWLHQLLGIMVQREASDLLISVNAPPTLKMAGRLTPLGDQGLSVAQVNELVGASLPEAQRERFTTEHEANFALSLK
DMGRFRISAFQQRNQMAMVVRRIGFDIPHLEELSLPPALGELVNNKRGLVFVVGGTGTGKSTTLASMIQQRNETIGGHII
SVEDPIEYVHPHRKAIINQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRETMEYALTFAETGHLCLATLHANNANQA
LDRILNFFPMERHAQVWLDLSLNLHAIVAQQLLPTIDGGRCPAIEIMLKSPLIGDLVRKGEVGEIKKIMARSRDLGMQTF
DQALFDLYKAGKISEEVALVHADSANDLRMMIKFGDEKSDGVKGAKESASHFSLRGEDDF

Nucleotide

• Length: 1143 bp

>NTDB_id=393888 FIU83_RS16485 WP_152485023.1 3621185..3622327(-) (pilU) [Halomonas sp. THAF5a]
ATGACGGCAAAAGAGTGGCTCCACCAGCTGCTGGGCATCATGGTGCAGAGGGAGGCGTCGGATCTGCTGATCTCGGTGAA
CGCGCCGCCCACCCTGAAGATGGCCGGCCGGCTCACGCCCCTGGGCGATCAGGGGCTCAGCGTGGCGCAGGTCAACGAGC
TGGTCGGCGCCTCGCTGCCCGAGGCGCAGCGGGAGCGCTTCACGACCGAGCACGAGGCCAACTTCGCGCTGAGCCTCAAG
GACATGGGGCGCTTTCGCATCAGCGCCTTCCAGCAGCGCAACCAGATGGCCATGGTCGTGCGGCGTATCGGCTTCGATAT
CCCCCACCTGGAGGAGCTCTCGCTGCCCCCCGCCCTTGGCGAGCTGGTCAACAACAAGCGGGGGCTGGTGTTCGTGGTCG
GCGGCACCGGCACCGGCAAGTCGACCACCCTGGCGTCGATGATCCAGCAGCGCAACGAGACGATCGGCGGCCATATCATA
AGCGTCGAGGACCCCATCGAGTACGTGCACCCGCATCGCAAGGCGATCATCAATCAGCGTGAGGTGGGCATCGACACCGA
GTCCTTCGAGGTGGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAGGTGCGCACCCGGGAGA
CCATGGAGTACGCCCTGACCTTCGCCGAGACCGGCCACCTCTGCCTGGCGACGCTGCACGCCAACAACGCCAACCAGGCG
CTGGATCGCATCCTCAACTTCTTCCCCATGGAGCGCCACGCCCAGGTGTGGCTCGATCTCTCGCTCAACCTGCACGCCAT
CGTCGCCCAGCAGCTGCTGCCCACGATCGACGGCGGGCGCTGCCCGGCGATCGAGATCATGCTCAAGTCGCCGCTGATCG
GCGACCTGGTGCGCAAGGGCGAGGTGGGCGAGATCAAGAAGATCATGGCGCGCTCGCGGGACCTCGGCATGCAGACCTTC
GACCAGGCGCTCTTCGACCTCTACAAGGCGGGCAAGATCAGCGAGGAGGTGGCGCTGGTGCACGCCGATTCGGCCAACGA
CCTGCGCATGATGATCAAGTTCGGCGACGAGAAAAGCGACGGGGTGAAGGGGGCCAAGGAGTCGGCCAGTCACTTCTCCC
TGCGGGGCGAGGACGACTTCTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-274)

3D structure

• AlphaFold DB: A0A5P9IT93

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.798	93.684	0.579
pilU	Vibrio cholerae strain A1552	57.263	94.211	0.539
pilU	Acinetobacter baylyi ADP1	55.807	92.895	0.518
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.71	96.316	0.392