Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FIU88_RS16265 Genome accession   NZ_CP045399
Coordinates   3498308..3499342 (-) Length   344 a.a.
NCBI ID   WP_152479875.1    Uniprot ID   A0A5P9HGL3
Organism   Halomonas sp. THAF12     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3493308..3504342
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIU88_RS16250 (FIU88_16290) tyrS 3494533..3495732 (-) 1200 WP_152479872.1 tyrosine--tRNA ligase -
  FIU88_RS16255 (FIU88_16295) - 3495844..3496974 (+) 1131 WP_152479873.1 anhydro-N-acetylmuramic acid kinase -
  FIU88_RS16260 (FIU88_16300) pilU 3497139..3498281 (-) 1143 WP_152479874.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FIU88_RS16265 (FIU88_16305) pilT 3498308..3499342 (-) 1035 WP_152479875.1 type IV pilus twitching motility protein PilT Machinery gene
  FIU88_RS16270 (FIU88_16310) - 3499397..3500104 (+) 708 WP_152479876.1 YggS family pyridoxal phosphate-dependent enzyme -
  FIU88_RS16275 (FIU88_16315) proC 3500170..3500994 (+) 825 WP_152479877.1 pyrroline-5-carboxylate reductase -
  FIU88_RS16280 (FIU88_16320) - 3501041..3501634 (+) 594 WP_016853956.1 YggT family protein -
  FIU88_RS16285 (FIU88_16325) - 3501913..3503070 (+) 1158 WP_152479878.1 homoserine O-acetyltransferase -
  FIU88_RS16290 (FIU88_16330) metW 3503070..3503663 (+) 594 WP_152480262.1 methionine biosynthesis protein MetW -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37918.42 Da        Isoelectric Point: 6.5965

>NTDB_id=393773 FIU88_RS16265 WP_152479875.1 3498308..3499342(-) (pilT) [Halomonas sp. THAF12]
MDITELLAFSAKQKASDLHLSAGLPPMIRVDGDIRRLNVPAMEDREVRKLIYDILTDRQRRDFEESLETDFSFEVPGVSR
FRVNAFHQARGAGAVFRTIPSEVMTMEDLGLGETFRRLAMLPRGLVLVTGPTGSGKSTTLAAMIDHINDNRFEHILTIED
PVEFVHRSKRCLINQREVHRDTQGFAPALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTVDRI
IDVFPGDEKAMVRSMLSESLQAVISQTLLKRQGGGRVAAHEILIATSAVRNLIREDKVAQIYSSLQTGGNLGMQTLDSAL
SRLVADNVVARDEAQAKARAVLAG

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=393773 FIU88_RS16265 WP_152479875.1 3498308..3499342(-) (pilT) [Halomonas sp. THAF12]
ATGGATATTACCGAACTGCTGGCATTCTCGGCAAAACAGAAGGCCTCGGATCTTCACCTTTCGGCCGGCCTGCCGCCGAT
GATCCGGGTCGATGGCGATATCCGCCGGCTCAACGTGCCGGCGATGGAGGATCGCGAGGTCCGCAAGCTGATCTACGACA
TCCTCACCGATCGTCAGCGGCGCGATTTCGAGGAGTCTCTGGAGACCGACTTCTCCTTCGAGGTGCCGGGGGTCTCGCGC
TTCCGTGTCAACGCCTTCCACCAGGCGCGCGGCGCCGGGGCGGTGTTCCGCACCATTCCCTCCGAGGTGATGACCATGGA
GGACCTGGGGCTGGGCGAGACGTTCCGCCGCCTGGCGATGCTGCCGCGCGGCCTGGTGCTGGTGACCGGCCCCACCGGTT
CGGGCAAGAGCACCACGCTGGCGGCGATGATCGATCACATCAACGACAACCGCTTCGAGCATATCCTCACCATCGAGGAT
CCGGTGGAGTTCGTGCATCGCAGCAAGCGCTGCCTGATCAACCAGCGCGAGGTGCATCGCGACACCCAGGGCTTCGCGCC
GGCGCTGCGCAGTGCCCTGCGCGAGGACCCTGACGTGATCCTGGTCGGCGAGATGCGCGACCTGGAGACCATCCGGCTGG
CGCTGACCGCGGCCGAGACCGGGCACCTGGTGTTCGGCACCCTGCACACCACCTCGGCGGCCAAGACCGTCGACCGGATC
ATCGACGTCTTCCCCGGCGACGAGAAGGCCATGGTGCGCTCGATGCTCTCGGAGTCGCTGCAGGCGGTGATCTCCCAGAC
CCTGCTCAAGCGCCAGGGCGGCGGCCGGGTGGCGGCCCACGAGATCCTGATCGCCACCTCGGCGGTGCGTAACCTGATTC
GCGAGGACAAGGTGGCGCAGATCTATTCGTCGCTGCAGACCGGCGGCAACCTGGGCATGCAGACCCTGGACTCGGCGCTG
TCGCGGCTGGTGGCGGACAACGTGGTGGCCCGGGACGAGGCCCAGGCCAAGGCGCGGGCCGTACTGGCCGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5P9HGL3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

78.761

98.547

0.776
  pilT Pseudomonas aeruginosa PAK

77.581

98.547

0.765
  pilT Acinetobacter baumannii D1279779

75.516

98.547

0.744
  pilT Acinetobacter baumannii strain A118

75.516

98.547

0.744
  pilT Acinetobacter nosocomialis M2

75.221

98.547

0.741
  pilT Acinetobacter baylyi ADP1

74.412

98.837

0.735
  pilT Legionella pneumophila strain Lp02

69.822

98.256

0.686
  pilT Legionella pneumophila strain ERS1305867

69.822

98.256

0.686
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.249

97.965

0.669
  pilT Vibrio cholerae strain A1552

68.249

97.965

0.669
  pilT Neisseria meningitidis 8013

66.471

98.837

0.657
  pilT Neisseria gonorrhoeae MS11

66.176

98.837

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.392

98.547

0.526
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Acinetobacter baylyi ADP1

43.168

93.605

0.404
  pilU Vibrio cholerae strain A1552

40.597

97.384

0.395

Multiple sequence alignment