Detailed information NTG0393772

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: FIU88_RS16260
• Genome accession: NZ_CP045399
• Coordinates: 3497139..3498281 (-)
• Length: 380 a.a.
• NCBI ID: WP_152479874.1
• Uniprot ID: A0A5P9HH08
• Organism: Halomonas sp. THAF12
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3492139..3503281

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FIU88_RS16250 (FIU88_16290)	tyrS	3494533..3495732 (-)	1200	WP_152479872.1	tyrosine--tRNA ligase	-
FIU88_RS16255 (FIU88_16295)	-	3495844..3496974 (+)	1131	WP_152479873.1	anhydro-N-acetylmuramic acid kinase	-
FIU88_RS16260 (FIU88_16300)	pilU	3497139..3498281 (-)	1143	WP_152479874.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
FIU88_RS16265 (FIU88_16305)	pilT	3498308..3499342 (-)	1035	WP_152479875.1	type IV pilus twitching motility protein PilT	Machinery gene
FIU88_RS16270 (FIU88_16310)	-	3499397..3500104 (+)	708	WP_152479876.1	YggS family pyridoxal phosphate-dependent enzyme	-
FIU88_RS16275 (FIU88_16315)	proC	3500170..3500994 (+)	825	WP_152479877.1	pyrroline-5-carboxylate reductase	-
FIU88_RS16280 (FIU88_16320)	-	3501041..3501634 (+)	594	WP_016853956.1	YggT family protein	-
FIU88_RS16285 (FIU88_16325)	-	3501913..3503070 (+)	1158	WP_152479878.1	homoserine O-acetyltransferase	-

Sequence

Protein

• Length: 380 a.a.
• Molecular weight: 42156.29 Da
• Isoelectric Point: 5.7280

>NTDB_id=393772 FIU88_RS16260 WP_152479874.1 3497139..3498281(-) (pilU) [Halomonas sp. THAF12]
MTAKEWLYQLLDIMIEQEASDLLISVGAPPTLKMAGKLMPMGEEGLSVAQVGELVAAAMPENHRARFDDEHEANFALSLS
GRGRFRVSAFRQRNQPAMVLRRIAFEIPHLEELSLPTVLGELANIKRGLVFVVGGTGTGKSTTLASMIQRRNETLGGHII
SIEDPIEYVHPHNKAIINQREVGIDTESFEVALKNTLRQAPDVILIGEIRTRETMEHALAFAETGHLCLATLHANNANQA
LDRIIHFFPHERHDQVWLDLSLNLQAIVAQQLLPTQAGGRCPAIEILLKSPLIADLVRKGEVSEIKNVMARSRDLGMQTF
DQALYDLYRKGQVSEEVVLVHADSANDLRMMIKYGDERGVGLAEAREAASHLSLRGEDDY

Nucleotide

• Length: 1143 bp

>NTDB_id=393772 FIU88_RS16260 WP_152479874.1 3497139..3498281(-) (pilU) [Halomonas sp. THAF12]
ATGACCGCGAAAGAATGGCTGTATCAGCTGCTCGACATCATGATCGAGCAGGAGGCCTCGGACCTGCTGATCTCGGTGGG
GGCGCCGCCGACGCTGAAGATGGCCGGCAAGCTGATGCCCATGGGCGAGGAGGGGCTGTCGGTGGCGCAGGTCGGCGAGC
TGGTGGCGGCGGCGATGCCGGAGAATCACCGGGCGCGCTTCGACGATGAACACGAGGCCAACTTCGCCCTCAGCCTGTCG
GGGCGGGGGCGCTTCCGCGTCAGCGCCTTCCGCCAGCGCAACCAGCCGGCCATGGTGCTGCGCCGGATCGCCTTCGAGAT
CCCGCACCTGGAGGAGCTGTCGCTGCCGACGGTGCTGGGCGAGCTGGCCAACATCAAGCGCGGGCTGGTGTTCGTGGTCG
GCGGCACCGGCACCGGCAAGTCGACGACCCTGGCGTCGATGATCCAGCGGCGTAACGAGACCCTCGGCGGCCATATCATC
AGCATCGAGGATCCGATCGAGTACGTTCACCCGCACAACAAGGCGATCATCAACCAGCGCGAGGTGGGCATCGATACCGA
GTCGTTCGAGGTGGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGCACCCGGGAGA
CCATGGAGCATGCCCTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCCAACAACGCCAACCAGGCG
CTGGACCGGATCATTCACTTCTTCCCCCACGAGCGGCACGATCAGGTGTGGCTCGACCTGTCGCTCAACCTGCAGGCCAT
CGTCGCCCAGCAGCTGTTGCCGACGCAGGCAGGGGGCCGCTGCCCGGCCATCGAGATCCTGCTGAAGTCGCCGCTGATCG
CCGACCTGGTCCGCAAGGGCGAGGTGTCCGAGATCAAGAACGTCATGGCCCGTTCCCGTGACCTGGGCATGCAGACCTTC
GACCAGGCGCTCTATGACCTCTACAGGAAGGGGCAGGTCAGCGAGGAGGTGGTACTGGTGCACGCCGACTCGGCCAACGA
CCTGCGCATGATGATCAAGTACGGCGACGAGCGCGGCGTGGGGCTGGCCGAGGCCAGGGAGGCGGCCAGCCATCTTTCGC
TGCGCGGCGAGGACGACTATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (119-274)

3D structure

• AlphaFold DB: A0A5P9HH08

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	64.571	92.105	0.595
pilU	Vibrio cholerae strain A1552	58.939	94.211	0.555
pilU	Acinetobacter baylyi ADP1	56.571	92.105	0.521
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	44.109	87.105	0.384
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.566	87.368	0.363
pilT	Vibrio cholerae strain A1552	41.566	87.368	0.363
pilT	Neisseria meningitidis 8013	41.265	87.368	0.361