Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FIU95_RS00870 Genome accession   NZ_CP045369
Coordinates   184314..185348 (-) Length   344 a.a.
NCBI ID   WP_152450613.1    Uniprot ID   A0A5P9ER06
Organism   Microbulbifer sp. THAF38     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 179314..190348
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIU95_RS00850 (FIU95_00850) argH 179613..181031 (-) 1419 WP_152450605.1 argininosuccinate lyase -
  FIU95_RS00855 (FIU95_00855) - 181121..182278 (+) 1158 WP_152450607.1 sensor histidine kinase -
  FIU95_RS00860 (FIU95_00860) - 182275..183015 (+) 741 WP_152450609.1 LytTR family DNA-binding domain-containing protein -
  FIU95_RS00865 (FIU95_00865) pilU 183115..184266 (-) 1152 WP_152450611.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FIU95_RS00870 (FIU95_00870) pilT 184314..185348 (-) 1035 WP_152450613.1 type IV pilus twitching motility protein PilT Machinery gene
  FIU95_RS00875 (FIU95_00875) - 185418..186122 (+) 705 WP_152450615.1 YggS family pyridoxal phosphate-dependent enzyme -
  FIU95_RS00880 (FIU95_00880) - 186196..186480 (+) 285 WP_152450617.1 hypothetical protein -
  FIU95_RS00885 (FIU95_00885) - 186540..188543 (-) 2004 WP_152450619.1 alpha/beta fold hydrolase -
  FIU95_RS00890 (FIU95_00890) proC 188685..189518 (+) 834 WP_253868782.1 pyrroline-5-carboxylate reductase -
  FIU95_RS00895 (FIU95_00895) - 189582..190190 (+) 609 WP_152450623.1 YggT family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38359.01 Da        Isoelectric Point: 6.5788

>NTDB_id=393694 FIU95_RS00870 WP_152450613.1 184314..185348(-) (pilT) [Microbulbifer sp. THAF38]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDVRRINLPAMEHKQVHGLIYEIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNHNRGAGAVFRTIPSRVLTMEDLGMGQVFKQISDTPRGLVLVTGPTGSGKSTTLAAMMDYINDNKYEHILTIED
PIEFVHESKKCLVNQREVHRDTHGFAEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAQEKAMVRSMLSESLQAVISQTLLKKNGGGRVAAHEIMRGTPAIRNLIREDKIAQMYSAIQTGASVGMQTMDQCL
EDLVARRIVSREVAREKAKMPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=393694 FIU95_RS00870 WP_152450613.1 184314..185348(-) (pilT) [Microbulbifer sp. THAF38]
ATGGATATCACTGAACTCCTCGCCTTCAGCGCCAAACAAAACGCTTCGGACCTGCACCTCTCCGCAGGCCTGCCGCCGAT
GATCCGTGTGGATGGCGATGTGCGCCGTATCAACCTGCCGGCCATGGAACACAAGCAGGTGCACGGCCTGATCTACGAGA
TCATGAACGACAAGCAACGCAAGGACTACGAAGAATTTCTCGAGACCGACTTCTCTTTCGAGGTGCCCGGTGTAGCGCGC
TTTCGTGTGAACGCCTTTAACCACAATCGCGGTGCCGGTGCAGTATTTCGGACCATTCCCTCCAGGGTTTTAACCATGGA
GGATCTGGGCATGGGCCAAGTTTTCAAACAGATCTCCGATACTCCGCGCGGCCTGGTCTTGGTCACAGGGCCCACCGGCT
CTGGTAAGTCCACCACCCTCGCCGCGATGATGGACTATATCAACGACAACAAATACGAGCATATCCTCACCATTGAGGAT
CCCATCGAATTCGTGCACGAATCCAAAAAATGCCTGGTGAACCAGCGCGAAGTGCATAGAGATACCCACGGCTTCGCAGA
AGCATTGCGCTCGGCCCTGCGTGAAGACCCGGATATCATTCTGGTGGGTGAGATGCGTGACCTGGAAACTATTCGCCTGG
CATTGACCGCAGCGGAAACCGGCCACTTGGTATTCGGCACCCTGCACACCACCTCCGCCGCCAAAACTATCGACCGGGTG
GTGGATGTGTTCCCGGCGCAGGAAAAAGCCATGGTGCGTTCCATGCTATCGGAATCCCTGCAGGCGGTAATCTCGCAGAC
ACTGCTGAAGAAAAACGGCGGTGGCCGTGTCGCCGCCCACGAAATCATGCGCGGCACCCCGGCAATCCGCAACCTGATCC
GCGAAGACAAAATCGCGCAGATGTACTCCGCCATCCAGACCGGTGCCAGCGTCGGTATGCAGACCATGGACCAGTGCCTG
GAAGACTTGGTGGCTCGCCGTATCGTCAGCCGCGAAGTCGCGCGGGAAAAAGCCAAGATGCCGGAAAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5P9ER06

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

88.953

100

0.89
  pilT Pseudomonas stutzeri DSM 10701

88.081

100

0.881
  pilT Acinetobacter baumannii D1279779

84.302

100

0.843
  pilT Acinetobacter baumannii strain A118

84.302

100

0.843
  pilT Acinetobacter nosocomialis M2

84.012

100

0.84
  pilT Acinetobacter baylyi ADP1

80.523

100

0.805
  pilT Legionella pneumophila strain Lp02

74.852

98.256

0.735
  pilT Legionella pneumophila strain ERS1305867

74.852

98.256

0.735
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.118

98.837

0.683
  pilT Vibrio cholerae strain A1552

69.118

98.837

0.683
  pilT Neisseria meningitidis 8013

67.826

100

0.68
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.547

0.401
  pilU Vibrio cholerae strain A1552

40.708

98.547

0.401
  pilU Acinetobacter baylyi ADP1

38.15

100

0.384

Multiple sequence alignment