Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FIU95_RS00865 Genome accession   NZ_CP045369
Coordinates   183115..184266 (-) Length   383 a.a.
NCBI ID   WP_152450611.1    Uniprot ID   -
Organism   Microbulbifer sp. THAF38     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 178115..189266
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIU95_RS00840 (FIU95_00840) - 178783..179103 (-) 321 WP_172975292.1 lipoprotein -
  FIU95_RS00845 (FIU95_00845) cyaY 179209..179532 (+) 324 WP_152450603.1 iron donor protein CyaY -
  FIU95_RS00850 (FIU95_00850) argH 179613..181031 (-) 1419 WP_152450605.1 argininosuccinate lyase -
  FIU95_RS00855 (FIU95_00855) - 181121..182278 (+) 1158 WP_152450607.1 sensor histidine kinase -
  FIU95_RS00860 (FIU95_00860) - 182275..183015 (+) 741 WP_152450609.1 LytTR family DNA-binding domain-containing protein -
  FIU95_RS00865 (FIU95_00865) pilU 183115..184266 (-) 1152 WP_152450611.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FIU95_RS00870 (FIU95_00870) pilT 184314..185348 (-) 1035 WP_152450613.1 type IV pilus twitching motility protein PilT Machinery gene
  FIU95_RS00875 (FIU95_00875) - 185418..186122 (+) 705 WP_152450615.1 YggS family pyridoxal phosphate-dependent enzyme -
  FIU95_RS00880 (FIU95_00880) - 186196..186480 (+) 285 WP_152450617.1 hypothetical protein -
  FIU95_RS00885 (FIU95_00885) - 186540..188543 (-) 2004 WP_152450619.1 alpha/beta fold hydrolase -

Sequence

Protein


Download         Length: 383 a.a.        Molecular weight: 42620.96 Da        Isoelectric Point: 6.5035

>NTDB_id=393693 FIU95_RS00865 WP_152450611.1 183115..184266(-) (pilU) [Microbulbifer sp. THAF38]
MDIERLLQLVVEKGASDLFITAGVPPSIKLHGRVVPCSSSALTPEKARELVVGTMNEKQRREFAEKKELNFAIAVRNVGR
FRVSAFFQRNLVGMVLRRIETKIPTIDGLGLPDVLKDLAMTKRGLIIFVGATGTGKSTSLASMIGHRNENTKGHIITIED
PIEFIHQHRGCIVTQREVGLDTDSFEIALKNTLRQAPDVILIGEVRSRETMEHAIAFAETGHLCLCTLHANNANQALDRI
IHFFPADRHQQLFMDLSLNLKAMVAQQLVPTPDGDGRRACLEIMINTPLMSDLVRKGQVHKMKELMKRSGEQGMQTFDQA
LYELYDRGEITYEDALAHADSANDLRLMIKLKSESDAEYLNSAAGELSLQNDSEYGDGGPQLY

Nucleotide


Download         Length: 1152 bp        

>NTDB_id=393693 FIU95_RS00865 WP_152450611.1 183115..184266(-) (pilU) [Microbulbifer sp. THAF38]
ATGGATATAGAAAGACTCCTACAGCTGGTGGTAGAGAAAGGTGCATCGGATCTGTTTATCACCGCCGGTGTACCGCCTTC
CATCAAACTGCACGGCCGCGTGGTGCCTTGCAGCAGCAGTGCACTCACTCCAGAAAAGGCACGCGAGCTGGTGGTGGGCA
CCATGAATGAGAAACAGCGTCGTGAGTTCGCCGAAAAAAAGGAATTAAACTTCGCCATCGCAGTGCGCAACGTAGGTCGT
TTCCGGGTGTCGGCTTTCTTCCAGCGCAACTTGGTGGGTATGGTGCTGCGACGCATCGAAACAAAAATCCCCACCATTGA
CGGCCTCGGCCTGCCGGATGTACTCAAAGATCTGGCCATGACCAAGCGCGGGCTGATCATTTTTGTGGGCGCCACCGGTA
CCGGTAAATCCACCTCCCTGGCCTCGATGATCGGTCACCGCAATGAGAACACCAAGGGTCATATCATCACCATTGAGGAC
CCGATCGAATTTATTCACCAGCATCGCGGCTGTATTGTGACCCAGCGCGAAGTGGGTCTGGACACCGACTCCTTCGAAAT
CGCTCTGAAGAACACCCTGCGTCAGGCGCCGGATGTCATTCTGATTGGTGAGGTGCGCTCTCGCGAGACGATGGAACACG
CCATTGCCTTCGCCGAGACCGGTCACCTGTGCCTGTGTACACTGCACGCCAACAATGCCAACCAGGCCCTCGACCGGATC
ATTCACTTCTTCCCGGCAGACCGTCACCAACAGCTGTTTATGGATCTATCCCTGAACCTTAAGGCCATGGTCGCCCAGCA
GCTGGTGCCAACACCGGATGGCGACGGCCGCCGCGCCTGTCTGGAAATTATGATCAATACACCGCTGATGTCAGACCTAG
TGCGCAAAGGCCAGGTGCACAAGATGAAGGAGTTGATGAAACGCTCCGGCGAACAGGGCATGCAGACCTTCGACCAGGCC
CTGTACGAACTCTACGATCGCGGCGAAATTACTTACGAGGACGCCCTCGCCCACGCCGACTCCGCCAACGACTTGCGCCT
GATGATCAAGCTCAAATCGGAATCCGATGCAGAGTATTTGAATAGCGCCGCCGGTGAGTTGAGTTTGCAAAATGACTCTG
AGTACGGCGATGGTGGACCGCAGCTTTACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

74.595

96.606

0.721
  pilU Acinetobacter baylyi ADP1

67.507

93.211

0.629
  pilU Vibrio cholerae strain A1552

54.717

96.867

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.018

88.512

0.407
  pilT Acinetobacter nosocomialis M2

43.881

87.467

0.384
  pilT Acinetobacter baumannii D1279779

43.881

87.467

0.384
  pilT Acinetobacter baumannii strain A118

43.881

87.467

0.384
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.515

87.206

0.371
  pilT Vibrio cholerae strain A1552

42.515

87.206

0.371
  pilT Acinetobacter baylyi ADP1

41.642

89.034

0.371
  pilT Pseudomonas aeruginosa PAK

42.09

87.467

0.368
  pilT Legionella pneumophila strain Lp02

41.791

87.467

0.366
  pilT Legionella pneumophila strain ERS1305867

41.791

87.467

0.366
  pilT Pseudomonas stutzeri DSM 10701

41.493

87.467

0.363

Multiple sequence alignment