Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FIU99_RS14330 Genome accession   NZ_CP045355
Coordinates   3097076..3098182 (-) Length   368 a.a.
NCBI ID   WP_039951288.1    Uniprot ID   A0AAJ4AIE2
Organism   Vibrio sp. THAF64     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3092076..3103182
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIU99_RS14300 (FIU99_14375) - 3092821..3093318 (+) 498 WP_006958245.1 SprT family zinc-dependent metalloprotease -
  FIU99_RS14305 (FIU99_14380) endA 3093398..3094102 (+) 705 WP_152472318.1 deoxyribonuclease I -
  FIU99_RS14310 (FIU99_14385) rsmE 3094211..3094942 (+) 732 WP_038509531.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  FIU99_RS14315 (FIU99_14390) gshB 3094956..3095906 (+) 951 WP_006958250.1 glutathione synthase -
  FIU99_RS14320 (FIU99_14395) - 3095963..3096526 (+) 564 WP_006958253.1 YqgE/AlgH family protein -
  FIU99_RS14325 (FIU99_14400) ruvX 3096544..3096966 (+) 423 WP_029236265.1 Holliday junction resolvase RuvX -
  FIU99_RS14330 (FIU99_14405) pilU 3097076..3098182 (-) 1107 WP_039951288.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FIU99_RS14335 (FIU99_14410) pilT 3098194..3099231 (-) 1038 WP_006958261.1 type IV pilus twitching motility protein PilT Machinery gene
  FIU99_RS14340 (FIU99_14415) - 3099256..3099972 (+) 717 WP_006958262.1 YggS family pyridoxal phosphate-dependent enzyme -
  FIU99_RS14345 (FIU99_14420) proC 3100000..3100818 (+) 819 WP_006958263.1 pyrroline-5-carboxylate reductase -
  FIU99_RS14350 (FIU99_14425) - 3100857..3101414 (+) 558 WP_006958264.1 YggT family protein -
  FIU99_RS14355 (FIU99_14430) yggU 3101414..3101704 (+) 291 WP_006958267.1 DUF167 family protein YggU -
  FIU99_RS14365 (FIU99_14440) - 3102267..3102776 (+) 510 WP_242704145.1 hypothetical protein -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41279.94 Da        Isoelectric Point: 5.1909

>NTDB_id=393619 FIU99_RS14330 WP_039951288.1 3097076..3098182(-) (pilU) [Vibrio sp. THAF64]
MDINPYLQGMLAEKASDLYITVGAPILYRVDGELKPHGENLSEAEVLTLLHSTMDEERQNDYSHSREANFAVMRDFGRFR
VSAFYQRELPGAVIRRIETTIPTFDDLKLPETLKDLSIAKRGLVLVVGATGSGKSTTMAAMTGYRNTERSGHILTVEDPI
EFVHEHQKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLKGVIGQQLIRDKDGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMAKSQEIGMQTFDQALY
QLVVNDKITEEDALHSADSANDLRIMLKTQRGESFGGGALADVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=393619 FIU99_RS14330 WP_039951288.1 3097076..3098182(-) (pilU) [Vibrio sp. THAF64]
ATGGATATCAATCCTTATCTGCAAGGCATGCTGGCTGAGAAAGCGTCAGACTTATACATCACTGTTGGTGCGCCGATACT
TTATCGTGTTGATGGTGAGTTGAAGCCGCATGGAGAGAACTTATCGGAAGCCGAAGTGCTGACGCTACTCCACAGCACCA
TGGATGAAGAGCGCCAGAATGACTATAGCCACAGTCGAGAAGCGAACTTTGCCGTAATGCGGGATTTTGGTCGTTTTCGT
GTTTCTGCTTTCTATCAGCGAGAATTACCCGGTGCGGTGATTCGTCGAATCGAAACCACCATACCCACATTTGATGACTT
AAAACTGCCTGAAACTCTGAAAGATCTTTCCATCGCCAAGCGCGGCTTGGTGCTAGTGGTGGGTGCAACAGGTTCCGGTA
AGTCGACCACTATGGCGGCGATGACCGGCTATCGAAATACTGAGCGTAGCGGTCATATTCTGACAGTCGAAGATCCGATT
GAATTTGTCCACGAACATCAGAAATGCATCGTCACGCAACGTGAGGTTGGCTTAGACACAGAAAGTTATGAAGTTGCACT
GAAGAACTCTTTGCGTCAGGCTCCTGATATGATTTTGATCGGTGAGATCCGCTCGCGAGAGACCATGGAGTACGCGATGA
CATTCGCCGAAACGGGGCATCTGTGTATGGCGACGTTGCACGCCAATAATGCTAACCAAGCTTTGGAAAGGATCCTACAC
CTTGTACCGAAAGAGCAAAAAGAGCAGTTCCTGTTTGATCTCTCCATGAACCTCAAAGGTGTTATCGGCCAGCAGTTGAT
CCGAGATAAAGACGGCCAAGGTCGTCATGGCGTATTTGAAATTCTACTTAACAGTCCACGTGTATCGGATCTCATTCGAC
GCGGTGATCTGCATGAACTCAAATCAACCATGGCGAAATCCCAGGAAATTGGTATGCAGACGTTTGATCAGGCGCTTTAC
CAACTGGTGGTGAATGACAAAATTACTGAAGAAGATGCGCTGCACAGTGCAGATTCAGCCAATGATCTGCGTATCATGCT
TAAAACTCAACGAGGTGAAAGCTTCGGTGGTGGTGCGCTGGCAGATGTGAAGATTGATATGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.065

100

0.821
  pilU Pseudomonas stutzeri DSM 10701

57.714

95.109

0.549
  pilU Acinetobacter baylyi ADP1

53.276

95.38

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.543

92.12

0.41
  pilT Legionella pneumophila strain Lp02

42.478

92.12

0.391
  pilT Legionella pneumophila strain ERS1305867

42.478

92.12

0.391
  pilT Acinetobacter baylyi ADP1

41.642

92.663

0.386
  pilT Acinetobacter nosocomialis M2

41.298

92.12

0.38
  pilT Pseudomonas aeruginosa PAK

41.298

92.12

0.38
  pilT Acinetobacter baumannii D1279779

41.298

92.12

0.38
  pilT Acinetobacter baumannii strain A118

41.298

92.12

0.38
  pilT Neisseria meningitidis 8013

39.42

93.75

0.37
  pilT Neisseria gonorrhoeae MS11

39.42

93.75

0.37
  pilT Pseudomonas stutzeri DSM 10701

39.823

92.12

0.367
  pilT Vibrio cholerae strain A1552

41.231

88.315

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.231

88.315

0.364

Multiple sequence alignment