Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FIU99_RS14335 Genome accession   NZ_CP045355
Coordinates   3098194..3099231 (-) Length   345 a.a.
NCBI ID   WP_006958261.1    Uniprot ID   -
Organism   Vibrio sp. THAF64     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3093194..3104231
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIU99_RS14305 (FIU99_14380) endA 3093398..3094102 (+) 705 WP_152472318.1 deoxyribonuclease I -
  FIU99_RS14310 (FIU99_14385) rsmE 3094211..3094942 (+) 732 WP_038509531.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  FIU99_RS14315 (FIU99_14390) gshB 3094956..3095906 (+) 951 WP_006958250.1 glutathione synthase -
  FIU99_RS14320 (FIU99_14395) - 3095963..3096526 (+) 564 WP_006958253.1 YqgE/AlgH family protein -
  FIU99_RS14325 (FIU99_14400) ruvX 3096544..3096966 (+) 423 WP_029236265.1 Holliday junction resolvase RuvX -
  FIU99_RS14330 (FIU99_14405) pilU 3097076..3098182 (-) 1107 WP_039951288.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FIU99_RS14335 (FIU99_14410) pilT 3098194..3099231 (-) 1038 WP_006958261.1 type IV pilus twitching motility protein PilT Machinery gene
  FIU99_RS14340 (FIU99_14415) - 3099256..3099972 (+) 717 WP_006958262.1 YggS family pyridoxal phosphate-dependent enzyme -
  FIU99_RS14345 (FIU99_14420) proC 3100000..3100818 (+) 819 WP_006958263.1 pyrroline-5-carboxylate reductase -
  FIU99_RS14350 (FIU99_14425) - 3100857..3101414 (+) 558 WP_006958264.1 YggT family protein -
  FIU99_RS14355 (FIU99_14430) yggU 3101414..3101704 (+) 291 WP_006958267.1 DUF167 family protein YggU -
  FIU99_RS14365 (FIU99_14440) - 3102267..3102776 (+) 510 WP_242704145.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38121.49 Da        Isoelectric Point: 6.1894

>NTDB_id=393620 FIU99_RS14335 WP_006958261.1 3098194..3099231(-) (pilT) [Vibrio sp. THAF64]
MDIAELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGVPAFNHSEVHRLVFEIMNDAQRSEFEERLEVDFSFALPDVGR
FRVNAFNQSRGCAAVFRTIPTDIPTLEQLEAPSIFENIANMEKGLILVTGPTGSGKSTTLAAMVDHINRHHNKHILTIED
PIEFVHTNNKCLINQREVHNDTHSFNNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKGMVRSMLSESLRAVIAQKLLKRIGGGRTACHEIMMATPAIRNLIREDKVAQMQSVIQTGAAHGMQTMEQHS
KQLIAQGLVDAEEVSKKIEVEQGLF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=393620 FIU99_RS14335 WP_006958261.1 3098194..3099231(-) (pilT) [Vibrio sp. THAF64]
ATGGATATCGCTGAATTACTGGATTTTAGTGTAAAGCATAATGCTTCAGATCTACATCTTTCTGCTGGTGTATCTCCCAT
GGTACGCATTGATGGTGAAGTAAGAAAGCTTGGCGTACCAGCGTTCAACCATTCCGAAGTGCATCGTTTGGTGTTTGAAA
TTATGAACGATGCTCAGCGTAGCGAATTTGAAGAACGTCTTGAAGTTGACTTTTCTTTTGCACTTCCGGACGTTGGCCGT
TTTAGGGTCAACGCTTTTAACCAATCTCGTGGTTGTGCGGCCGTATTTCGTACCATTCCAACCGATATCCCGACTTTAGA
GCAGCTAGAAGCGCCGTCGATCTTTGAAAATATCGCCAATATGGAGAAAGGATTAATCTTGGTCACTGGGCCAACGGGCT
CAGGTAAATCGACTACTTTGGCGGCAATGGTGGATCATATTAATCGTCACCACAATAAGCATATCCTGACTATTGAAGAT
CCGATTGAGTTTGTGCACACGAATAACAAATGTCTGATCAACCAGCGTGAAGTTCACAATGACACCCATAGTTTTAATAA
CGCGCTGCGCAGTGCATTGCGTGAAGATCCCGATGTCATTTTAGTTGGTGAGTTACGCGATCAGGAAACGATCAGTCTAG
CGCTGACCGCTGCTGAAACTGGCCACTTGGTATTTGGTACTTTGCACACCAGTTCGGCGGCGAAAACGATCGACCGTATC
ATTGATGTTTTCCCAGGTAGCGATAAAGGCATGGTGCGTTCCATGCTCTCTGAGTCTCTGCGTGCGGTTATTGCGCAGAA
ATTACTCAAGCGCATTGGCGGCGGGAGAACCGCTTGTCATGAAATCATGATGGCAACGCCTGCTATTCGCAACTTGATCC
GCGAAGACAAAGTCGCGCAAATGCAGTCTGTGATTCAGACTGGTGCAGCGCATGGTATGCAAACCATGGAGCAGCATTCG
AAACAGTTGATTGCTCAAGGTCTGGTTGATGCAGAAGAAGTGTCGAAAAAAATCGAAGTTGAGCAAGGTCTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

87.246

100

0.872
  pilT Vibrio cholerae strain A1552

87.246

100

0.872
  pilT Acinetobacter baylyi ADP1

68.713

99.13

0.681
  pilT Acinetobacter baumannii strain A118

69.277

96.232

0.667
  pilT Acinetobacter baumannii D1279779

69.277

96.232

0.667
  pilT Acinetobacter nosocomialis M2

68.976

96.232

0.664
  pilT Pseudomonas stutzeri DSM 10701

66.766

97.681

0.652
  pilT Pseudomonas aeruginosa PAK

65.497

99.13

0.649
  pilT Legionella pneumophila strain Lp02

66.869

95.362

0.638
  pilT Legionella pneumophila strain ERS1305867

66.869

95.362

0.638
  pilT Neisseria meningitidis 8013

64.497

97.971

0.632
  pilT Neisseria gonorrhoeae MS11

63.235

98.551

0.623
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.818

95.652

0.496
  pilU Vibrio cholerae strain A1552

42.462

94.203

0.4
  pilU Pseudomonas stutzeri DSM 10701

40.12

96.812

0.388
  pilU Acinetobacter baylyi ADP1

41.25

92.754

0.383

Multiple sequence alignment