Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FIV01_RS01975 Genome accession   NZ_CP045350
Coordinates   406438..407544 (+) Length   368 a.a.
NCBI ID   WP_152429492.1    Uniprot ID   A0A5P9CFX0
Organism   Vibrio aquimaris strain THAF100     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 401438..412544
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIV01_RS01945 (FIV01_01935) - 401968..402573 (-) 606 WP_152429487.1 XTP/dITP diphosphatase -
  FIV01_RS01950 (FIV01_01940) - 402602..403033 (-) 432 WP_152429488.1 DUF4426 domain-containing protein -
  FIV01_RS01955 (FIV01_01945) - 403209..403766 (-) 558 WP_152429489.1 YggT family protein -
  FIV01_RS01960 (FIV01_01950) proC 403806..404624 (-) 819 WP_152429490.1 pyrroline-5-carboxylate reductase -
  FIV01_RS01965 (FIV01_01955) - 404636..405364 (-) 729 WP_152429491.1 YggS family pyridoxal phosphate-dependent enzyme -
  FIV01_RS01970 (FIV01_01960) pilT 405389..406426 (+) 1038 WP_114787444.1 type IV pilus twitching motility protein PilT Machinery gene
  FIV01_RS01975 (FIV01_01965) pilU 406438..407544 (+) 1107 WP_152429492.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FIV01_RS01980 (FIV01_01970) ruvX 407563..407985 (-) 423 WP_152429493.1 Holliday junction resolvase RuvX -
  FIV01_RS01985 (FIV01_01975) - 408003..408566 (-) 564 WP_152429494.1 YqgE/AlgH family protein -
  FIV01_RS01990 (FIV01_01980) gshB 408620..409570 (-) 951 WP_152429495.1 glutathione synthase -
  FIV01_RS01995 (FIV01_01985) rsmE 409582..410313 (-) 732 WP_152429496.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  FIV01_RS02000 (FIV01_01990) - 410623..411234 (+) 612 WP_152429497.1 LysE family translocator -
  FIV01_RS02005 (FIV01_01995) - 411249..411737 (-) 489 WP_152429498.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41164.92 Da        Isoelectric Point: 5.9363

>NTDB_id=393561 FIV01_RS01975 WP_152429492.1 406438..407544(+) (pilU) [Vibrio aquimaris strain THAF100]
MDINACLQGMLSEKASDLYITVDAPLLYRVNGELKPLGDTLSSQAVFTLLHSMMDEERQKEYAQSREANFAVVKDFGRFR
VSAFFQRELPGAVIRRIETSIPTFEQLKLPETLRDLSIAKRGLVLVVGATGSGKSTTMAAMTGYRNHHRSGHILTVEDPI
EFVHEHDKCIVTQREVGLDTDSYEVALKNSLRQAPDMILIGEIRSRETMEFAMSFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVIAQQLLRDKNGNGRHGVFEILLNSPRVSDLIRRGDLHELKATMAKSQQSGMQTFDQALY
QLVIDDKITEDDALHSADSANDLRIMLKTNRGDSFGGGALSDVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=393561 FIV01_RS01975 WP_152429492.1 406438..407544(+) (pilU) [Vibrio aquimaris strain THAF100]
ATGGATATCAATGCTTGCTTACAAGGGATGTTGAGTGAAAAGGCATCGGATTTATATATCACTGTCGATGCACCTCTTCT
CTATCGGGTGAACGGGGAATTGAAACCCCTTGGTGATACTTTGTCATCTCAAGCGGTATTCACTTTGTTACACAGCATGA
TGGATGAGGAGCGTCAAAAGGAATATGCCCAAAGCAGAGAAGCCAATTTTGCTGTCGTGAAAGATTTTGGCCGTTTTAGG
GTCTCGGCATTTTTCCAACGTGAGCTACCTGGTGCTGTCATTCGACGTATCGAGACGAGTATTCCGACTTTTGAGCAATT
GAAACTCCCAGAAACCTTAAGAGATTTGTCGATTGCCAAACGTGGGTTGGTTTTAGTAGTGGGTGCGACAGGTTCTGGTA
AATCGACCACTATGGCGGCCATGACAGGGTATCGGAATCATCATCGAAGTGGGCATATCCTTACCGTTGAGGATCCCATA
GAGTTTGTACATGAACACGATAAATGCATTGTTACTCAACGTGAAGTTGGTCTAGATACCGACAGTTATGAAGTGGCGTT
GAAGAATTCTCTTAGGCAGGCCCCAGATATGATTTTGATCGGTGAAATACGATCTCGAGAAACGATGGAGTTTGCGATGA
GTTTTGCTGAAACGGGTCATTTGTGTATGGCGACGTTACACGCAAACAATGCCAATCAAGCGCTTGAACGTATTCTTCAC
CTTGTACCTAAAGAGCAAAAAGAGCAGTTTTTATTCGATTTGTCGATGAACTTACGAGGTGTGATCGCTCAACAATTGCT
TCGCGATAAAAATGGCAATGGTCGACATGGTGTGTTTGAAATCTTGCTAAATAGCCCAAGAGTCTCGGATCTGATCCGAC
GCGGCGATCTTCATGAGCTTAAAGCGACCATGGCTAAATCCCAACAATCGGGCATGCAGACGTTTGATCAGGCGCTGTAT
CAGTTGGTTATCGATGATAAGATCACAGAAGATGATGCGCTTCACAGTGCTGATTCAGCGAATGATTTGCGCATCATGCT
CAAAACGAACCGAGGGGATAGTTTTGGTGGTGGTGCTTTATCCGACGTGAAAATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5P9CFX0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.065

100

0.821
  pilU Pseudomonas stutzeri DSM 10701

56.571

95.109

0.538
  pilU Acinetobacter baylyi ADP1

52.137

95.38

0.497
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.068

92.12

0.397
  pilT Legionella pneumophila strain ERS1305867

41.888

92.12

0.386
  pilT Legionella pneumophila strain Lp02

41.888

92.12

0.386
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.462

88.315

0.375
  pilT Vibrio cholerae strain A1552

42.462

88.315

0.375
  pilT Acinetobacter baylyi ADP1

42.154

88.315

0.372
  pilT Acinetobacter baumannii strain A118

40.413

92.12

0.372
  pilT Acinetobacter baumannii D1279779

40.413

92.12

0.372
  pilT Pseudomonas stutzeri DSM 10701

39.823

92.12

0.367
  pilT Pseudomonas aeruginosa PAK

39.823

92.12

0.367
  pilT Neisseria gonorrhoeae MS11

39.13

93.75

0.367
  pilT Neisseria meningitidis 8013

39.13

93.75

0.367
  pilT Acinetobacter nosocomialis M2

41.231

88.315

0.364

Multiple sequence alignment