Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FIV01_RS01970 Genome accession   NZ_CP045350
Coordinates   405389..406426 (+) Length   345 a.a.
NCBI ID   WP_114787444.1    Uniprot ID   -
Organism   Vibrio aquimaris strain THAF100     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 400389..411426
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIV01_RS01940 (FIV01_01930) hemW 400778..401956 (-) 1179 WP_152429486.1 radical SAM family heme chaperone HemW -
  FIV01_RS01945 (FIV01_01935) - 401968..402573 (-) 606 WP_152429487.1 XTP/dITP diphosphatase -
  FIV01_RS01950 (FIV01_01940) - 402602..403033 (-) 432 WP_152429488.1 DUF4426 domain-containing protein -
  FIV01_RS01955 (FIV01_01945) - 403209..403766 (-) 558 WP_152429489.1 YggT family protein -
  FIV01_RS01960 (FIV01_01950) proC 403806..404624 (-) 819 WP_152429490.1 pyrroline-5-carboxylate reductase -
  FIV01_RS01965 (FIV01_01955) - 404636..405364 (-) 729 WP_152429491.1 YggS family pyridoxal phosphate-dependent enzyme -
  FIV01_RS01970 (FIV01_01960) pilT 405389..406426 (+) 1038 WP_114787444.1 type IV pilus twitching motility protein PilT Machinery gene
  FIV01_RS01975 (FIV01_01965) pilU 406438..407544 (+) 1107 WP_152429492.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FIV01_RS01980 (FIV01_01970) ruvX 407563..407985 (-) 423 WP_152429493.1 Holliday junction resolvase RuvX -
  FIV01_RS01985 (FIV01_01975) - 408003..408566 (-) 564 WP_152429494.1 YqgE/AlgH family protein -
  FIV01_RS01990 (FIV01_01980) gshB 408620..409570 (-) 951 WP_152429495.1 glutathione synthase -
  FIV01_RS01995 (FIV01_01985) rsmE 409582..410313 (-) 732 WP_152429496.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  FIV01_RS02000 (FIV01_01990) - 410623..411234 (+) 612 WP_152429497.1 LysE family translocator -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 37891.33 Da        Isoelectric Point: 6.1084

>NTDB_id=393560 FIV01_RS01970 WP_114787444.1 405389..406426(+) (pilT) [Vibrio aquimaris strain THAF100]
MDIAELLDFSVKHNASDLHLSAGVSPMIRIDGEVRKLGVPALSHSEVHRLISDIMSDSQRSEFEEHLEVDFSFELPDVGR
FRVNAFNQSRGCSAVFRTIPTVIPTLEQLEAPAIFESISNMEKGLILVTGPTGSGKSTTLAAMVDYINRNHNKHILTIED
PIEFVHTNNKCLLNQREVHKDTHSFNNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPGSDKGMVRSMLSESLRAVIAQKLLKKVGGGRVACHEIMMATPAIRNLIREDKVAQMQSVIQTGAAHGMQTMEQHS
KQLIAQGLVDAEEVAKKIEAESGMM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=393560 FIV01_RS01970 WP_114787444.1 405389..406426(+) (pilT) [Vibrio aquimaris strain THAF100]
ATGGATATTGCTGAATTACTGGACTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCAGGTGTATCCCCAAT
GATACGTATTGATGGAGAAGTCAGAAAGCTTGGAGTACCAGCATTGAGTCATTCTGAGGTTCATCGATTAATCTCAGATA
TTATGAGTGACTCCCAGCGTAGCGAATTTGAGGAACATTTAGAAGTTGATTTTTCATTTGAACTTCCAGATGTTGGTCGT
TTTCGTGTTAATGCGTTTAATCAATCGCGAGGATGCTCTGCTGTATTTCGGACTATACCTACTGTGATCCCCACTTTAGA
ACAATTGGAAGCGCCTGCGATCTTTGAAAGTATTTCTAATATGGAAAAAGGTTTGATCCTTGTGACAGGACCAACTGGAT
CAGGCAAATCTACGACACTGGCTGCCATGGTTGATTACATTAATCGCAACCACAACAAACATATTTTGACCATTGAAGAT
CCAATTGAATTTGTACACACCAATAACAAATGTCTGTTAAACCAGCGTGAAGTACATAAAGATACTCACAGTTTCAACAA
TGCCTTGCGTAGTGCTTTACGTGAGGATCCTGATGTTATTTTGGTTGGTGAGCTTAGAGATCAAGAAACAATAAGTCTTG
CTCTTACTGCAGCGGAAACAGGGCACCTTGTGTTTGGTACCTTACACACCAGTTCAGCTGCGAAAACCGTTGACCGTATC
ATTGATGTATTTCCAGGTAGTGATAAAGGTATGGTTCGCTCTATGCTCTCAGAATCACTCAGAGCCGTTATTGCACAGAA
GCTACTAAAGAAAGTGGGCGGGGGCCGAGTAGCTTGCCATGAGATTATGATGGCGACGCCAGCGATTAGAAACCTAATCC
GTGAAGACAAAGTCGCTCAGATGCAATCTGTAATTCAAACCGGTGCGGCGCATGGTATGCAGACGATGGAGCAGCATTCG
AAGCAATTGATTGCTCAGGGACTTGTTGATGCTGAAGAAGTGGCGAAGAAAATTGAAGCTGAATCTGGAATGATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

87.316

98.261

0.858
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

87.316

98.261

0.858
  pilT Acinetobacter baylyi ADP1

69.679

99.42

0.693
  pilT Acinetobacter baumannii D1279779

69.388

99.42

0.69
  pilT Acinetobacter baumannii strain A118

69.388

99.42

0.69
  pilT Acinetobacter nosocomialis M2

69.096

99.42

0.687
  pilT Pseudomonas stutzeri DSM 10701

68.713

99.13

0.681
  pilT Pseudomonas aeruginosa PAK

68.249

97.681

0.667
  pilT Legionella pneumophila strain Lp02

68.389

95.362

0.652
  pilT Legionella pneumophila strain ERS1305867

68.389

95.362

0.652
  pilT Neisseria meningitidis 8013

64.793

97.971

0.635
  pilT Neisseria gonorrhoeae MS11

62.609

100

0.626
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.606

95.652

0.484
  pilU Vibrio cholerae strain A1552

42.462

94.203

0.4
  pilU Pseudomonas stutzeri DSM 10701

40.12

96.812

0.388
  pilU Acinetobacter baylyi ADP1

40.625

92.754

0.377

Multiple sequence alignment