Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FIV04_RS12730 Genome accession   NZ_CP045338
Coordinates   2848890..2849927 (-) Length   345 a.a.
NCBI ID   WP_150869306.1    Uniprot ID   A0A5P9BBF5
Organism   Vibrio sp. THAF190c     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2843890..2854927
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIV04_RS12705 (FIV04_12745) rsmE 2844647..2845378 (+) 732 WP_032549841.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  FIV04_RS12710 (FIV04_12750) gshB 2845392..2846342 (+) 951 WP_152469315.1 glutathione synthase -
  FIV04_RS12715 (FIV04_12755) - 2846437..2847000 (+) 564 WP_032549843.1 YqgE/AlgH family protein -
  FIV04_RS12720 (FIV04_12760) ruvX 2847032..2847454 (+) 423 WP_032549845.1 Holliday junction resolvase RuvX -
  FIV04_RS26935 (FIV04_12765) - 2847551..2847709 (+) 159 WP_172974247.1 hypothetical protein -
  FIV04_RS12725 (FIV04_12770) pilU 2847765..2848874 (-) 1110 WP_152469316.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FIV04_RS12730 (FIV04_12775) pilT 2848890..2849927 (-) 1038 WP_150869306.1 type IV pilus twitching motility protein PilT Machinery gene
  FIV04_RS12735 (FIV04_12780) - 2849954..2850658 (+) 705 WP_150869307.1 YggS family pyridoxal phosphate-dependent enzyme -
  FIV04_RS12740 (FIV04_12785) proC 2850715..2851533 (+) 819 WP_152469317.1 pyrroline-5-carboxylate reductase -
  FIV04_RS12745 (FIV04_12790) - 2851588..2852145 (+) 558 WP_150869309.1 YggT family protein -
  FIV04_RS12750 (FIV04_12795) yggU 2852145..2852435 (+) 291 WP_150869310.1 DUF167 family protein YggU -
  FIV04_RS12755 (FIV04_12800) - 2852464..2852895 (+) 432 WP_152469318.1 DUF4426 domain-containing protein -
  FIV04_RS12760 (FIV04_12805) - 2852934..2853533 (+) 600 WP_152469319.1 XTP/dITP diphosphatase -
  FIV04_RS12765 (FIV04_12810) hemW 2853533..2854720 (+) 1188 WP_152469320.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38219.74 Da        Isoelectric Point: 6.1877

>NTDB_id=393502 FIV04_RS12730 WP_150869306.1 2848890..2849927(-) (pilT) [Vibrio sp. THAF190c]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMNDSQRSEFEEKLEVDFSFELPNVGR
FRVNAFNQSRGCSAVFRTIPVEIPTLEQLGAPDIFEKIANFEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLVNQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLIAQGLVDSDEVQKKIEIETALF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=393502 FIV04_RS12730 WP_150869306.1 2848890..2849927(-) (pilT) [Vibrio sp. THAF190c]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTTAGGAAGCTTGGAATACCGGCTTTGAGCCATGCTGATGTTCACCGTTTGGTTTTTGAGA
TCATGAACGATTCACAGCGCAGTGAGTTTGAAGAGAAGCTGGAAGTGGACTTTTCATTTGAGTTGCCGAATGTTGGCCGT
TTCCGTGTGAATGCCTTTAACCAGTCGCGTGGTTGCTCTGCGGTGTTTCGAACCATCCCTGTTGAAATCCCGACGCTCGA
GCAGTTAGGTGCTCCTGATATCTTTGAAAAGATAGCTAACTTTGAGAAAGGTTTGGTTTTGGTCACTGGCCCAACGGGCT
CTGGTAAGTCGACCACACTGGCGGCGATGGTTGATTATGTAAACCGTAACCACAACAAGCACATCCTGACCATTGAAGAC
CCTATCGAATTTGTTCATACCAATAACAAGTGCTTGGTTAACCAACGTGAGGTTCATCGTGATACTCACAGCTTTAAAGC
GGCGCTGCGTAGTGCACTGCGTGAAGACCCAGACGTCATCTTAGTTGGCGAGCTGCGTGACCAAGAGACAATCAGCTTAG
CATTGACCGCGGCAGAAACGGGTCACCTAGTCTTTGGTACTCTGCATACTAGTTCTGCGGCCAAAACCATCGACCGTATT
ATTGATGTGTTCCCAGGCAGTGATAAAGACATGGTTCGCTCGATGCTTTCTGAATCCTTGCGTTCGGTGATTGCTCAGAA
GCTGCTTAAGCGTGTTGGTGGTGGACGAGTCGCGTGTCATGAAATCATGATGGCGACCCCTGCGATTCGTAACTTGATTC
GTGAGGACAAGGTCGCTCAGATGTATTCAATTATTCAAACAGGTGCCGCGCATGGTATGCAGACCATGGAGCAAAATGCC
AAACAGTTGATTGCCCAAGGCCTAGTTGACTCAGATGAAGTCCAGAAGAAAATTGAAATAGAAACAGCGCTGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5P9BBF5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

89.565

100

0.896
  pilT Vibrio cholerae strain A1552

89.565

100

0.896
  pilT Acinetobacter baumannii D1279779

72.948

95.362

0.696
  pilT Acinetobacter baumannii strain A118

72.948

95.362

0.696
  pilT Acinetobacter nosocomialis M2

72.644

95.362

0.693
  pilT Pseudomonas stutzeri DSM 10701

70.03

97.681

0.684
  pilT Acinetobacter baylyi ADP1

71.212

95.652

0.681
  pilT Pseudomonas aeruginosa PAK

68.824

98.551

0.678
  pilT Legionella pneumophila strain Lp02

68.997

95.362

0.658
  pilT Legionella pneumophila strain ERS1305867

68.997

95.362

0.658
  pilT Neisseria meningitidis 8013

63.235

98.551

0.623
  pilT Neisseria gonorrhoeae MS11

62.941

98.551

0.62
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.198

96.812

0.496
  pilU Vibrio cholerae strain A1552

43.077

94.203

0.406
  pilU Pseudomonas stutzeri DSM 10701

42.073

95.072

0.4
  pilU Acinetobacter baylyi ADP1

39.688

92.754

0.368

Multiple sequence alignment