Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FIV04_RS12725 Genome accession   NZ_CP045338
Coordinates   2847765..2848874 (-) Length   369 a.a.
NCBI ID   WP_152469316.1    Uniprot ID   -
Organism   Vibrio sp. THAF190c     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2842765..2853874
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIV04_RS12700 (FIV04_12740) - 2843112..2844452 (-) 1341 WP_152469314.1 hypothetical protein -
  FIV04_RS12705 (FIV04_12745) rsmE 2844647..2845378 (+) 732 WP_032549841.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  FIV04_RS12710 (FIV04_12750) gshB 2845392..2846342 (+) 951 WP_152469315.1 glutathione synthase -
  FIV04_RS12715 (FIV04_12755) - 2846437..2847000 (+) 564 WP_032549843.1 YqgE/AlgH family protein -
  FIV04_RS12720 (FIV04_12760) ruvX 2847032..2847454 (+) 423 WP_032549845.1 Holliday junction resolvase RuvX -
  FIV04_RS26935 (FIV04_12765) - 2847551..2847709 (+) 159 WP_172974247.1 hypothetical protein -
  FIV04_RS12725 (FIV04_12770) pilU 2847765..2848874 (-) 1110 WP_152469316.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FIV04_RS12730 (FIV04_12775) pilT 2848890..2849927 (-) 1038 WP_150869306.1 type IV pilus twitching motility protein PilT Machinery gene
  FIV04_RS12735 (FIV04_12780) - 2849954..2850658 (+) 705 WP_150869307.1 YggS family pyridoxal phosphate-dependent enzyme -
  FIV04_RS12740 (FIV04_12785) proC 2850715..2851533 (+) 819 WP_152469317.1 pyrroline-5-carboxylate reductase -
  FIV04_RS12745 (FIV04_12790) - 2851588..2852145 (+) 558 WP_150869309.1 YggT family protein -
  FIV04_RS12750 (FIV04_12795) yggU 2852145..2852435 (+) 291 WP_150869310.1 DUF167 family protein YggU -
  FIV04_RS12755 (FIV04_12800) - 2852464..2852895 (+) 432 WP_152469318.1 DUF4426 domain-containing protein -
  FIV04_RS12760 (FIV04_12805) - 2852934..2853533 (+) 600 WP_152469319.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41424.08 Da        Isoelectric Point: 5.0950

>NTDB_id=393501 FIV04_RS12725 WP_152469316.1 2847765..2848874(-) (pilU) [Vibrio sp. THAF190c]
MELNQILEGMLSQKASDLYITVDAPVLFRVDGELRPQGEVLNEDQVSQLLDAMMDQERRDEYRQTREANFAIVRDGGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNNNRSGHILTVEDPI
EFVHEHKKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVQKEQKEQFLFDLSMNLRGVVAQQLIRDKNGNGRHGVFEILLNSPRVSDLIRRGDLHELKATMAKSKEIGMQTFDQALY
DLVIAGEISEEDAFHSADSANDLRLMLKTQRGDDDYGTGSLAGVKIDMG

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=393501 FIV04_RS12725 WP_152469316.1 2847765..2848874(-) (pilU) [Vibrio sp. THAF190c]
ATGGAATTAAATCAGATCCTTGAGGGGATGCTGTCGCAAAAGGCGTCCGATCTTTATATCACGGTTGATGCGCCAGTGTT
GTTTCGTGTCGATGGAGAGCTGCGCCCGCAAGGTGAAGTTCTCAATGAAGATCAAGTGTCACAGCTGCTAGACGCAATGA
TGGATCAAGAGCGACGTGATGAGTATCGCCAAACGCGTGAGGCGAACTTTGCCATTGTTCGAGACGGTGGGCGCTTTCGT
GTCAGTGCTTTCTTTCAGCGAGAACTGCCAGGTGCTGTGATTCGTCGTATTGAAACCAACATCCCAACCTTTGAGCAATT
AAAGCTCCCTGATGTGCTACAGGACCTTTCAATCGCTAAACGCGGCCTTGTGCTGGTGGTTGGTGCAACAGGTTCTGGTA
AGTCGACGTCAATGGCGGCAATGACGGGTTATCGCAACAACAATCGTTCAGGTCATATTCTTACGGTGGAAGATCCGATT
GAGTTTGTGCATGAGCACAAGAAGTGCATTGTCACCCAGCGTGAGGTGGGCTTAGATACAGAGAGCTACGAAGTTGCGCT
CAAGAACTCGTTACGTCAAGCTCCCGATATGATCTTGATTGGTGAGATTCGAAGCCGTGAAACCATGGAATACGCAATGA
CCTTTGCTGAGACTGGGCACTTATGTATGGCCACTCTGCATGCGAATAATGCTAACCAAGCTTTAGAACGTATTCTTCAT
TTGGTGCAGAAAGAGCAGAAAGAGCAGTTCTTGTTTGATTTGTCGATGAACCTGCGTGGAGTTGTTGCACAGCAGCTCAT
ACGAGACAAAAATGGCAATGGTCGTCACGGTGTGTTTGAGATTTTACTCAACAGCCCACGTGTGTCAGATCTTATTCGTC
GTGGTGATTTGCATGAACTCAAAGCAACCATGGCAAAGTCGAAAGAGATTGGTATGCAGACTTTCGATCAAGCGCTGTAT
GACTTAGTTATTGCAGGGGAAATCAGTGAAGAGGATGCATTCCACAGTGCAGACTCGGCTAACGACTTGCGATTGATGCT
GAAAACACAACGCGGTGACGATGATTACGGCACTGGATCTCTGGCTGGTGTTAAGATTGATATGGGATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

83.424

99.729

0.832
  pilU Pseudomonas stutzeri DSM 10701

55.795

100

0.561
  pilU Acinetobacter baylyi ADP1

53.846

95.122

0.512
  pilT Legionella pneumophila strain Lp02

42.398

92.683

0.393
  pilT Legionella pneumophila strain ERS1305867

42.398

92.683

0.393
  pilT Acinetobacter baumannii strain A118

42.059

92.141

0.388
  pilT Acinetobacter baumannii D1279779

42.059

92.141

0.388
  pilT Acinetobacter nosocomialis M2

42.059

92.141

0.388
  pilT Acinetobacter baylyi ADP1

41.813

92.683

0.388
  pilT Pseudomonas aeruginosa PAK

41.791

90.786

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.284

87.805

0.371
  pilT Pseudomonas stutzeri DSM 10701

40.299

90.786

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.515

0.363
  pilT Vibrio cholerae strain A1552

40.12

90.515

0.363
  pilT Neisseria gonorrhoeae MS11

38.218

94.309

0.36
  pilT Neisseria meningitidis 8013

38.218

94.309

0.36

Multiple sequence alignment