Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GCU53_RS11250 Genome accession   NZ_CP045302
Coordinates   2417161..2418195 (-) Length   344 a.a.
NCBI ID   WP_152387695.1    Uniprot ID   -
Organism   Azotobacter salinestris strain KACC 13899     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2412161..2423195
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCU53_RS11225 pyrR 2412606..2413115 (+) 510 WP_152387690.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  GCU53_RS11230 - 2413128..2414144 (+) 1017 WP_152387691.1 aspartate carbamoyltransferase catalytic subunit -
  GCU53_RS11235 - 2414144..2415415 (+) 1272 WP_152387692.1 dihydroorotase -
  GCU53_RS11240 - 2415488..2415889 (-) 402 WP_152387693.1 NINE protein -
  GCU53_RS11245 pilU 2415971..2417116 (-) 1146 WP_152387694.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GCU53_RS11250 pilT 2417161..2418195 (-) 1035 WP_152387695.1 type IV pilus twitching motility protein PilT Machinery gene
  GCU53_RS11255 - 2418267..2418959 (+) 693 WP_152387696.1 YggS family pyridoxal phosphate-dependent enzyme -
  GCU53_RS11260 proC 2418977..2419795 (+) 819 WP_152387697.1 pyrroline-5-carboxylate reductase -
  GCU53_RS11265 - 2419809..2420402 (+) 594 WP_152387698.1 YggT family protein -
  GCU53_RS11270 - 2420402..2420701 (+) 300 WP_152387699.1 DUF167 domain-containing protein -
  GCU53_RS11275 - 2420773..2421912 (+) 1140 WP_152387700.1 homoserine O-acetyltransferase -
  GCU53_RS11280 metW 2421920..2422513 (+) 594 WP_152387701.1 methionine biosynthesis protein MetW -
  GCU53_RS11285 - 2422576..2422995 (+) 420 WP_152387702.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38088.92 Da        Isoelectric Point: 6.8890

>NTDB_id=393277 GCU53_RS11250 WP_152387695.1 2417161..2418195(-) (pilT) [Azotobacter salinestris strain KACC 13899]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPMDHKQVHALIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSRVLSMDDLGMGEVFKRITEVPRGLVLVTGPTGSGKSTTLAAMLDYLNANKHHHILTIED
PIEFVHESKKCLINQREVHRDTLGFAEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKVGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGSIGMQTLDMCL
KNLLSRGMITRESAREKAKIPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=393277 GCU53_RS11250 WP_152387695.1 2417161..2418195(-) (pilT) [Azotobacter salinestris strain KACC 13899]
ATGGACATTACCGAGCTGCTCGCCTTCAGCGCCAAGCAGGGCGCGTCGGACCTGCACCTCTCCGCCGGTCTGCCGCCGAT
GATTCGCGTCGACGGCGACGTGCGCCGCATCAACCTGCCGCCGATGGATCACAAGCAGGTGCACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTACGAGGAGTTTCTCGAAACCGACTTCTCCTTCGAGGTGCCCGGCGTGGCGCGC
TTCCGGGTCAACGCCTTCAACCAGAACCGCGGTGCCGGCGCGGTGTTCCGGACCATTCCCTCGCGCGTGCTGAGCATGGA
CGATCTGGGGATGGGCGAGGTGTTCAAGAGGATCACCGAGGTGCCGCGCGGCCTGGTGCTGGTCACCGGCCCCACCGGCT
CGGGCAAGTCGACCACCCTGGCGGCCATGCTCGACTATCTGAACGCCAACAAGCACCACCACATCCTCACCATCGAGGAC
CCGATCGAATTCGTCCACGAATCGAAGAAGTGCCTGATCAACCAGCGCGAGGTGCACCGCGATACCCTGGGCTTCGCCGA
GGCGCTGCGTTCGGCGCTGCGCGAGGACCCGGACGTCATCCTGGTGGGCGAGATGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCACCTCGGCGGCCAAGACCATCGACCGGGTG
GTCGACGTGTTCCCCGCGGAAGAGAAGTCGATGGTCCGCTCGATGCTCTCCGAATCGCTGCAGGCGGTGATTTCCCAGAC
CCTGCTGAAGAAGGTCGGTGGCGGCCGGGTGGCGGCCCACGAGATCATGATCGGCACCCCGGCGATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCGATCCAGACCGGCGGCTCGATCGGCATGCAGACCCTGGACATGTGCCTG
AAGAACCTGCTGTCCAGGGGCATGATCACCCGCGAGAGCGCCCGCGAGAAGGCGAAGATTCCCGACAACTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

92.151

100

0.922
  pilT Pseudomonas aeruginosa PAK

91.57

100

0.916
  pilT Acinetobacter nosocomialis M2

83.14

100

0.831
  pilT Acinetobacter baumannii D1279779

82.849

100

0.828
  pilT Acinetobacter baumannii strain A118

82.849

100

0.828
  pilT Acinetobacter baylyi ADP1

81.105

100

0.811
  pilT Legionella pneumophila strain Lp02

73.669

98.256

0.724
  pilT Legionella pneumophila strain ERS1305867

73.669

98.256

0.724
  pilT Neisseria meningitidis 8013

68.824

98.837

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.529

98.837

0.677
  pilT Vibrio cholerae strain A1552

68.529

98.837

0.677
  pilT Neisseria gonorrhoeae MS11

68.529

98.837

0.677
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.526
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398
  pilU Vibrio cholerae strain A1552

39.528

98.547

0.39

Multiple sequence alignment