Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GCU53_RS11245 Genome accession   NZ_CP045302
Coordinates   2415971..2417116 (-) Length   381 a.a.
NCBI ID   WP_152387694.1    Uniprot ID   -
Organism   Azotobacter salinestris strain KACC 13899     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2410971..2422116
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCU53_RS11215 - 2411565..2412137 (+) 573 WP_152387688.1 YqgE/AlgH family protein -
  GCU53_RS11220 ruvX 2412134..2412562 (+) 429 WP_152387689.1 Holliday junction resolvase RuvX -
  GCU53_RS11225 pyrR 2412606..2413115 (+) 510 WP_152387690.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  GCU53_RS11230 - 2413128..2414144 (+) 1017 WP_152387691.1 aspartate carbamoyltransferase catalytic subunit -
  GCU53_RS11235 - 2414144..2415415 (+) 1272 WP_152387692.1 dihydroorotase -
  GCU53_RS11240 - 2415488..2415889 (-) 402 WP_152387693.1 NINE protein -
  GCU53_RS11245 pilU 2415971..2417116 (-) 1146 WP_152387694.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GCU53_RS11250 pilT 2417161..2418195 (-) 1035 WP_152387695.1 type IV pilus twitching motility protein PilT Machinery gene
  GCU53_RS11255 - 2418267..2418959 (+) 693 WP_152387696.1 YggS family pyridoxal phosphate-dependent enzyme -
  GCU53_RS11260 proC 2418977..2419795 (+) 819 WP_152387697.1 pyrroline-5-carboxylate reductase -
  GCU53_RS11265 - 2419809..2420402 (+) 594 WP_152387698.1 YggT family protein -
  GCU53_RS11270 - 2420402..2420701 (+) 300 WP_152387699.1 DUF167 domain-containing protein -
  GCU53_RS11275 - 2420773..2421912 (+) 1140 WP_152387700.1 homoserine O-acetyltransferase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42992.35 Da        Isoelectric Point: 6.8865

>NTDB_id=393276 GCU53_RS11245 WP_152387694.1 2415971..2417116(-) (pilU) [Azotobacter salinestris strain KACC 13899]
MEFEKLLRLMMEKGASDLFITAGMPPSMKLNGRLVPVTRHPLSPEQARETVFAVMNEQQRREFAATHECNFAISARGIGR
FRVSAFYQRNLMGMVLRRIETRIPTFDELKLPEVLRELSMTKRGLVLFVGATGTGKSTSLAAMIGYRNQNSSGHIISIED
PIEFIHQHQGCIVTQREVGIDTDSFEVALKNTLRQAPDVIMVGEIRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPADRHRQVWMDLSLNLRAIVAQQLIPTPDGKGRRAVIEVLINTPLVADLIRKGEVHELKAVMKRSTEQGMQTFDQA
LYQLYSQGEITYEEALRHADSANDLRLMIKLESETDSRHLESVAEGLSLQASEEDTGRRMR

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=393276 GCU53_RS11245 WP_152387694.1 2415971..2417116(-) (pilU) [Azotobacter salinestris strain KACC 13899]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGATGGAGAAGGGCGCCTCCGATCTGTTCATCACTGCGGGCATGCCGCCGTC
GATGAAGCTCAACGGGCGCCTGGTGCCGGTGACCCGCCACCCGCTGTCGCCGGAGCAGGCCCGGGAAACCGTGTTCGCGG
TGATGAACGAGCAGCAGCGTCGCGAGTTCGCCGCGACTCACGAATGCAACTTCGCCATCAGCGCCCGCGGTATCGGCCGC
TTCCGGGTCAGCGCCTTCTACCAGCGCAACCTGATGGGCATGGTGCTGCGCCGCATCGAGACGCGCATCCCGACCTTCGA
CGAGCTCAAGCTGCCCGAGGTGCTGCGCGAGCTGTCGATGACCAAGCGCGGGCTGGTGCTGTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTATCGCAACCAGAACAGCAGCGGCCACATCATTTCCATCGAGGAT
CCCATCGAGTTCATCCACCAGCATCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGCATCGATACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGGTCGGCGAGATCCGCACTCGCGAGACCATGGACTACG
CGGTGGCCTTCGCCGAAACCGGCCACCTGTGTCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTCGACCGGATC
ATCAACTTCTTCCCGGCCGACCGCCACCGCCAGGTATGGATGGACCTGTCGCTGAACCTGCGCGCCATCGTCGCCCAGCA
GTTGATCCCGACGCCGGACGGCAAGGGGCGCCGGGCGGTGATCGAGGTGCTGATCAATACCCCGCTGGTCGCCGACCTGA
TCCGCAAGGGCGAGGTCCACGAGCTCAAGGCGGTGATGAAGCGCTCCACCGAACAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACAGCCAGGGCGAGATCACCTACGAGGAAGCCCTGCGCCATGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTGGAGTCGGAGACCGACAGCCGGCACCTGGAGAGCGTGGCCGAGGGCCTCAGCCTGCAGGCCAGCGAGG
AGGACACCGGCCGGCGCATGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

83.727

100

0.837
  pilU Acinetobacter baylyi ADP1

64.706

93.701

0.606
  pilU Vibrio cholerae strain A1552

60.112

93.438

0.562
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.953

88.976

0.391

Multiple sequence alignment