Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GBK02_RS00460 Genome accession   NZ_CP045202
Coordinates   90219..91262 (-) Length   347 a.a.
NCBI ID   WP_203467827.1    Uniprot ID   -
Organism   Dechloromonas sp. TW-R-39-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 85219..96262
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBK02_RS00435 (GBK02_00435) - 85403..85747 (-) 345 WP_203467822.1 hypothetical protein -
  GBK02_RS00440 (GBK02_00440) - 85886..87877 (-) 1992 WP_203467823.1 UvrD-helicase domain-containing protein -
  GBK02_RS00445 (GBK02_00445) - 87976..88293 (-) 318 WP_203467824.1 hypothetical protein -
  GBK02_RS00450 (GBK02_00450) - 88342..89028 (-) 687 WP_203467825.1 DUF4197 domain-containing protein -
  GBK02_RS00455 (GBK02_00455) pilU 89052..90188 (-) 1137 WP_203467826.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GBK02_RS00460 (GBK02_00460) pilT 90219..91262 (-) 1044 WP_203467827.1 type IV pilus twitching motility protein PilT Machinery gene
  GBK02_RS00465 (GBK02_00465) - 91316..92005 (+) 690 WP_203467828.1 YggS family pyridoxal phosphate-dependent enzyme -
  GBK02_RS00470 (GBK02_00470) proC 92002..92814 (+) 813 WP_203467829.1 pyrroline-5-carboxylate reductase -
  GBK02_RS00475 (GBK02_00475) - 92817..93350 (+) 534 WP_203467830.1 YggT family protein -
  GBK02_RS00480 (GBK02_00480) - 93358..93651 (+) 294 WP_203467831.1 DUF167 domain-containing protein -
  GBK02_RS00485 (GBK02_00485) - 93703..94041 (-) 339 WP_203467832.1 ComEA family DNA-binding protein -
  GBK02_RS00490 (GBK02_00490) - 94181..95434 (-) 1254 WP_203467833.1 dihydroorotase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38422.10 Da        Isoelectric Point: 7.1069

>NTDB_id=392650 GBK02_RS00460 WP_203467827.1 90219..91262(-) (pilT) [Dechloromonas sp. TW-R-39-2]
MDITELLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINLPPMEHKDVHGMVYDIMNDGQRKVYEETLECDFSFEIPNLAR
FRVNAFNQQRGAGAVFRTIPTKVMTLEELNCPKIFREISEYPRGIVLVTGPTGSGKSTTLAAMVNHVNENHYGHILTVED
PIEFVHDAKKCLINQREVGPHTLSFSNALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRI
VDVFPAAEKEMVRSMLSESLRAVISQTLLKTKDGAGRVAAHEIMIGTPAIRNLIRENKVAQMYSAIQTGQNVGMQTLDQN
LLDMVRRGIVSSNDARNKASNKDAFPA

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=392650 GBK02_RS00460 WP_203467827.1 90219..91262(-) (pilT) [Dechloromonas sp. TW-R-39-2]
ATGGACATTACCGAACTGCTGGCTTTCAGCGTCAAGAACAAGGCTTCCGACTTGCACCTGTCGGCCGGCCTGCCGCCCAT
GATCCGGGTGCACGGCGATGTTCGGCGGATCAACCTGCCGCCGATGGAGCACAAGGATGTTCATGGCATGGTCTATGACA
TCATGAATGACGGCCAGCGCAAGGTCTATGAGGAAACGCTGGAGTGCGATTTCTCCTTCGAAATTCCCAACCTGGCGCGT
TTCCGGGTCAATGCCTTCAACCAGCAGCGTGGTGCCGGTGCCGTTTTCCGGACCATTCCGACCAAGGTCATGACGCTGGA
AGAACTCAACTGTCCGAAGATTTTCCGCGAAATTTCCGAGTATCCGCGCGGTATCGTGCTGGTTACCGGGCCGACCGGTT
CGGGCAAGTCGACGACGCTGGCGGCGATGGTCAATCACGTCAATGAAAACCACTATGGCCACATCCTGACGGTCGAGGAC
CCGATCGAATTTGTGCACGATGCCAAGAAATGCCTGATCAATCAGCGCGAAGTCGGGCCGCATACGCTGTCTTTCTCGAA
CGCCCTGCGCTCGGCGCTGCGCGAAGACCCGGACGTGATCCTGGTCGGTGAAATGCGCGACCTCGAAACCATCCGTCTGG
CACTGACGGCGGCCGAAACCGGCCACCTGGTATTTGGCACGCTGCATACTTCTTCGGCCGCCAAGACGGTTGACCGTATC
GTTGACGTTTTCCCGGCGGCTGAAAAGGAAATGGTTCGCTCGATGCTGTCCGAATCGCTGCGCGCCGTCATTTCGCAGAC
CCTGCTCAAGACCAAGGATGGCGCCGGCCGCGTGGCTGCCCACGAAATCATGATTGGCACGCCGGCCATCCGCAACCTGA
TCCGTGAAAACAAGGTGGCGCAGATGTATTCTGCGATCCAGACCGGCCAGAATGTCGGCATGCAGACGCTGGATCAGAAT
CTGCTCGACATGGTTCGGCGCGGCATTGTTTCAAGCAACGATGCACGCAACAAGGCATCGAACAAGGATGCTTTCCCGGC
CTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

77.971

99.424

0.775
  pilT Acinetobacter baumannii strain A118

77.971

99.424

0.775
  pilT Acinetobacter nosocomialis M2

77.971

99.424

0.775
  pilT Pseudomonas aeruginosa PAK

75.942

99.424

0.755
  pilT Acinetobacter baylyi ADP1

75.942

99.424

0.755
  pilT Pseudomonas stutzeri DSM 10701

75.072

99.424

0.746
  pilT Neisseria meningitidis 8013

71.594

99.424

0.712
  pilT Legionella pneumophila strain Lp02

71.594

99.424

0.712
  pilT Legionella pneumophila strain ERS1305867

71.594

99.424

0.712
  pilT Neisseria gonorrhoeae MS11

71.304

99.424

0.709
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.118

97.406

0.683
  pilT Vibrio cholerae strain A1552

70.118

97.406

0.683
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

99.424

0.51
  pilU Acinetobacter baylyi ADP1

40.936

98.559

0.403
  pilU Vibrio cholerae strain A1552

41.056

98.271

0.403
  pilU Pseudomonas stutzeri DSM 10701

41.791

96.542

0.403

Multiple sequence alignment