Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GBK02_RS00455 Genome accession   NZ_CP045202
Coordinates   89052..90188 (-) Length   378 a.a.
NCBI ID   WP_203467826.1    Uniprot ID   A0A974Y644
Organism   Dechloromonas sp. TW-R-39-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 84052..95188
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBK02_RS00425 (GBK02_00425) - 84412..84594 (+) 183 WP_203467820.1 hypothetical protein -
  GBK02_RS00430 (GBK02_00430) - 84821..85270 (+) 450 WP_203467821.1 cytochrome c5 family protein -
  GBK02_RS00435 (GBK02_00435) - 85403..85747 (-) 345 WP_203467822.1 hypothetical protein -
  GBK02_RS00440 (GBK02_00440) - 85886..87877 (-) 1992 WP_203467823.1 UvrD-helicase domain-containing protein -
  GBK02_RS00445 (GBK02_00445) - 87976..88293 (-) 318 WP_203467824.1 hypothetical protein -
  GBK02_RS00450 (GBK02_00450) - 88342..89028 (-) 687 WP_203467825.1 DUF4197 domain-containing protein -
  GBK02_RS00455 (GBK02_00455) pilU 89052..90188 (-) 1137 WP_203467826.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GBK02_RS00460 (GBK02_00460) pilT 90219..91262 (-) 1044 WP_203467827.1 type IV pilus twitching motility protein PilT Machinery gene
  GBK02_RS00465 (GBK02_00465) - 91316..92005 (+) 690 WP_203467828.1 YggS family pyridoxal phosphate-dependent enzyme -
  GBK02_RS00470 (GBK02_00470) proC 92002..92814 (+) 813 WP_203467829.1 pyrroline-5-carboxylate reductase -
  GBK02_RS00475 (GBK02_00475) - 92817..93350 (+) 534 WP_203467830.1 YggT family protein -
  GBK02_RS00480 (GBK02_00480) - 93358..93651 (+) 294 WP_203467831.1 DUF167 domain-containing protein -
  GBK02_RS00485 (GBK02_00485) - 93703..94041 (-) 339 WP_203467832.1 ComEA family DNA-binding protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42199.59 Da        Isoelectric Point: 6.5070

>NTDB_id=392649 GBK02_RS00455 WP_203467826.1 89052..90188(-) (pilU) [Dechloromonas sp. TW-R-39-2]
MERDQAMKFMHDLLRLMSQKKGSDLFIAAGFAPAIKIDGKVTPVSNQVLTAQHTAELARSIMNDRQAAEFEATKECNFAI
SPSGIGRFRVNALVQQGRVGVVCRTINMTIPTLDELQMPPVLKDLAMSKRGLIIFVGGTGTGKTTSLAALVDYRNENSFG
HIITIEDPIEYVHEHKNCIVTQREVGVDTDDWGPALKNTLRQAPDVILMGEIRDRNTMDYAIAFAETGHLALATLHANSA
NQAIDRIINFFPEERRQQLLMDLSLNMRAMVSQRLLPKKDGKGRVAAIEVMLNSPLIADLIFRGEVQEIKEIMKKSRELG
MQTFDQALFDLYEAGKISYEDALRNADSLNDLRLQIKLYGKESKDRDLSSGLNHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=392649 GBK02_RS00455 WP_203467826.1 89052..90188(-) (pilU) [Dechloromonas sp. TW-R-39-2]
ATGGAACGCGATCAGGCAATGAAGTTCATGCACGATCTTTTGCGCCTGATGTCGCAAAAGAAAGGCTCCGATTTGTTCAT
CGCCGCCGGTTTTGCGCCGGCCATCAAGATCGATGGCAAGGTGACGCCGGTGTCGAACCAGGTATTGACGGCGCAACACA
CGGCCGAGTTGGCCCGCTCGATCATGAACGACCGCCAGGCGGCCGAGTTCGAGGCGACCAAGGAGTGCAATTTCGCCATT
TCACCCTCCGGTATCGGGCGCTTCCGGGTCAATGCGCTGGTTCAGCAAGGTCGCGTCGGTGTCGTATGCCGGACGATCAA
CATGACGATTCCGACGCTCGATGAGTTGCAAATGCCGCCGGTGCTCAAGGATCTGGCGATGAGCAAGCGCGGCCTGATCA
TCTTCGTCGGCGGCACCGGCACCGGCAAGACGACCTCGCTGGCTGCCTTGGTCGATTATCGCAACGAGAATTCCTTCGGC
CACATCATCACCATCGAAGACCCGATCGAATATGTACACGAGCACAAGAACTGCATCGTGACGCAGCGCGAAGTCGGCGT
TGATACCGACGACTGGGGTCCGGCCCTGAAGAACACCCTGCGCCAGGCACCGGATGTCATCCTGATGGGCGAAATCCGCG
ACCGCAACACGATGGATTACGCCATCGCCTTTGCCGAAACCGGCCACCTGGCGCTGGCTACGCTGCATGCCAACAGCGCC
AACCAGGCGATCGACCGGATCATCAATTTCTTCCCCGAAGAGCGTCGCCAGCAATTGCTGATGGATCTGTCGCTCAATAT
GCGGGCCATGGTTTCCCAGCGCCTGCTGCCCAAGAAAGACGGCAAGGGGCGGGTTGCCGCGATCGAGGTAATGCTCAATT
CGCCGCTGATCGCCGACCTGATCTTCCGCGGCGAGGTGCAGGAGATCAAGGAAATCATGAAGAAGTCGCGCGAGCTGGGC
ATGCAGACCTTCGACCAGGCCCTGTTCGATCTCTACGAAGCCGGCAAAATCAGCTATGAAGATGCGTTGCGCAACGCCGA
TTCGCTCAATGACCTGCGCTTGCAGATCAAGTTGTATGGCAAGGAATCGAAGGATCGAGACCTGAGCAGCGGTCTGAACC
ATCTCGATATCGTCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(113-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.088

96.296

0.598
  pilU Acinetobacter baylyi ADP1

59.944

94.444

0.566
  pilU Vibrio cholerae strain A1552

54.839

90.212

0.495
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.582

88.624

0.386
  pilT Pseudomonas aeruginosa PAK

42.687

88.624

0.378
  pilT Acinetobacter baylyi ADP1

41.543

89.153

0.37
  pilT Pseudomonas stutzeri DSM 10701

41.791

88.624

0.37
  pilT Legionella pneumophila strain ERS1305867

41.692

87.566

0.365
  pilT Legionella pneumophila strain Lp02

41.692

87.566

0.365
  pilT Acinetobacter nosocomialis M2

41.39

87.566

0.362
  pilT Acinetobacter baumannii D1279779

41.39

87.566

0.362
  pilT Acinetobacter baumannii strain A118

41.39

87.566

0.362

Multiple sequence alignment