Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GBN68_RS12965 Genome accession   NZ_CP045101
Coordinates   2664695..2665732 (+) Length   345 a.a.
NCBI ID   WP_234954122.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain AJ210     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2659695..2670732
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBN68_RS12955 (GBN68_12975) - 2662964..2663698 (-) 735 WP_148826638.1 hypothetical protein -
  GBN68_RS12960 (GBN68_12980) - 2663883..2664572 (-) 690 WP_062034236.1 YggS family pyridoxal phosphate-dependent enzyme -
  GBN68_RS12965 (GBN68_12985) pilT 2664695..2665732 (+) 1038 WP_234954122.1 type IV pilus twitching motility protein PilT Machinery gene
  GBN68_RS12970 (GBN68_12990) pilU 2665759..2666880 (+) 1122 WP_004694864.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GBN68_RS12975 (GBN68_12995) fur 2666941..2667378 (-) 438 WP_004694866.1 ferric iron uptake transcriptional regulator -
  GBN68_RS12980 (GBN68_13000) - 2667491..2667889 (+) 399 WP_004694868.1 outer membrane protein assembly factor BamE -
  GBN68_RS12985 (GBN68_13005) - 2667936..2668259 (-) 324 WP_104222298.1 RnfH family protein -
  GBN68_RS12990 (GBN68_13010) - 2668259..2669341 (-) 1083 WP_234954124.1 hypothetical protein -
  GBN68_RS12995 (GBN68_13015) - 2669484..2669939 (-) 456 WP_005400160.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38305.02 Da        Isoelectric Point: 6.6658

>NTDB_id=391916 GBN68_RS12965 WP_234954122.1 2664695..2665732(+) (pilT) [Acinetobacter johnsonii strain AJ210]
MDITELLAFSAKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKEVHKLVYDIMNDKQRRDFEEKLETDFSFEVPSIAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQTFKELCELPRGLVLVTGPTGSGKSTTLAAMIDYINDNRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KNLVIKGVISPQTARTAAKQPETFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=391916 GBN68_RS12965 WP_234954122.1 2664695..2665732(+) (pilT) [Acinetobacter johnsonii strain AJ210]
ATGGATATCACAGAATTACTAGCCTTTTCGGCTAAGAACGGGGCATCAGATTTACACTTATCTGCAGGTATGCCACCGAT
GATTCGTGTCGATGGTGAAGTTCGTCGTATCAATTTACCAGCTTTAGAACACAAAGAAGTGCATAAACTGGTCTATGACA
TCATGAATGACAAACAACGTCGTGATTTCGAAGAAAAATTAGAAACGGACTTTTCCTTTGAAGTGCCAAGCATTGCTCGC
TTTCGTGTGAACGCATTCAACCAAAACCGTGGTGCAGGTGCAGTATTCCGTACCATTCCATCCAAAGTCCTGACCATGGA
AGACTTAGGTTTAGGACAAACCTTTAAAGAGTTATGTGAATTGCCACGTGGTTTAGTTTTAGTGACGGGGCCTACTGGTT
CTGGTAAATCTACCACGTTGGCTGCAATGATCGATTATATTAACGATAACCGCTATGACCATATCTTAACGGTTGAAGAC
CCAATCGAATTTGTACACCAGTCAAAAAAATGCTTGATCAACCAACGTGAAGTGCACCGTGATACACATGGTTTTAACGA
AGCGCTGCGTTCAGCACTACGTGAAGACCCCGATATTATCCTCGTGGGTGAGATGCGTGACCTTGAAACCATTCGTTTGG
CGCTCACTGCCGCTGAAACGGGTCACTTGGTCTTCGGTACACTACATACCACCTCTGCGGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCTGAAGAAAAAGACATGGTTCGTGCCATGTTATCTGAATCGCTACAAGCCGTGATTTCTCAAAC
CTTACTCAAGAAAAATGGCGGTGGCCGTGTTGCCGCGCATGAAATCATGATTGGTATTCCTGCCATTCGTAACTTAATTC
GTGAAAATAAAGTGGCACAGATGTATTCTGCTATTCAGACTGGTGCAAACTACGGTATGACCACACTGGATCAAAGTTTG
AAAAACTTGGTCATCAAAGGGGTGATCAGTCCACAAACTGCCCGTACAGCTGCCAAGCAACCTGAAACCTTCTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

94.493

100

0.945
  pilT Acinetobacter baumannii D1279779

94.493

100

0.945
  pilT Acinetobacter baumannii strain A118

94.493

100

0.945
  pilT Acinetobacter baylyi ADP1

86.337

99.71

0.861
  pilT Pseudomonas aeruginosa PAK

84.012

99.71

0.838
  pilT Pseudomonas stutzeri DSM 10701

83.43

99.71

0.832
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Neisseria meningitidis 8013

67.246

100

0.672
  pilT Neisseria gonorrhoeae MS11

66.957

100

0.67
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.452

97.391

0.667
  pilT Vibrio cholerae strain A1552

68.452

97.391

0.667
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.525
  pilU Vibrio cholerae strain A1552

42.183

98.261

0.414
  pilU Pseudomonas stutzeri DSM 10701

42.09

97.101

0.409
  pilU Acinetobacter baylyi ADP1

39.942

99.42

0.397

Multiple sequence alignment