Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GBN68_RS12970 Genome accession   NZ_CP045101
Coordinates   2665759..2666880 (+) Length   373 a.a.
NCBI ID   WP_004694864.1    Uniprot ID   A0A380UB92
Organism   Acinetobacter johnsonii strain AJ210     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2660759..2671880
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBN68_RS12955 (GBN68_12975) - 2662964..2663698 (-) 735 WP_148826638.1 hypothetical protein -
  GBN68_RS12960 (GBN68_12980) - 2663883..2664572 (-) 690 WP_062034236.1 YggS family pyridoxal phosphate-dependent enzyme -
  GBN68_RS12965 (GBN68_12985) pilT 2664695..2665732 (+) 1038 WP_234954122.1 type IV pilus twitching motility protein PilT Machinery gene
  GBN68_RS12970 (GBN68_12990) pilU 2665759..2666880 (+) 1122 WP_004694864.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GBN68_RS12975 (GBN68_12995) fur 2666941..2667378 (-) 438 WP_004694866.1 ferric iron uptake transcriptional regulator -
  GBN68_RS12980 (GBN68_13000) - 2667491..2667889 (+) 399 WP_004694868.1 outer membrane protein assembly factor BamE -
  GBN68_RS12985 (GBN68_13005) - 2667936..2668259 (-) 324 WP_104222298.1 RnfH family protein -
  GBN68_RS12990 (GBN68_13010) - 2668259..2669341 (-) 1083 WP_234954124.1 hypothetical protein -
  GBN68_RS12995 (GBN68_13015) - 2669484..2669939 (-) 456 WP_005400160.1 bacteriohemerythrin -
  GBN68_RS13000 (GBN68_13020) - 2670214..2671062 (-) 849 WP_184118212.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41844.10 Da        Isoelectric Point: 6.5786

>NTDB_id=391917 GBN68_RS12970 WP_004694864.1 2665759..2666880(+) (pilU) [Acinetobacter johnsonii strain AJ210]
MDFNDLLNYMIQQKASDLFITADVEPSMKINGQIVPVAKGKLTGDVAGQLLNSIMSEKQRKEFADTHECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETQIPTMDELKLPPVLKELAMTKRGIIIFVGATGTGKSTSLASIIGYRNQNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHSQLFMDLSLNLKAMVAQQLIPTPDGNSRRAAIEILINSPLISDLIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAKQITYKDALKHADSPNDLRLQIKLSEEGGDRLLHASANITFDGQA

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=391917 GBN68_RS12970 WP_004694864.1 2665759..2666880(+) (pilU) [Acinetobacter johnsonii strain AJ210]
ATGGACTTTAACGACTTACTCAACTATATGATTCAACAAAAAGCGTCAGATTTATTTATCACCGCTGATGTTGAACCCTC
CATGAAAATTAACGGTCAAATTGTGCCTGTGGCTAAGGGCAAGCTGACGGGTGATGTCGCGGGGCAACTACTCAACTCGA
TTATGAGTGAAAAGCAGCGTAAAGAATTTGCAGACACACATGAGTGCAACTTCGCGATTATGAATCGGGATAAAACTGCA
CGTTTTCGTGTCAGCGCATTTCAACAGCGTGATATGCCAGGTATGGTTTTGCGTCGAATTGAGACGCAAATTCCAACCAT
GGATGAGTTAAAACTCCCACCTGTGCTCAAAGAGTTAGCCATGACCAAACGTGGCATCATCATCTTTGTGGGTGCAACAG
GTACGGGTAAATCAACTTCTTTGGCTTCGATCATTGGTTACCGTAACCAAAACTCGAAAGGTCATATCATTACCATTGAA
GACCCAATTGAATTTATTCATGAGCATGCAGGCTGTATCATTACCCAGCGTGAAGTGGGCATTGATACGGACTCTTTTGA
AATCGCGCTGAAAAATACCTTACGTCAGGCACCCGATGTGATCCTGATTGGTGAGATTCGTTCCCGCGAAACCATGGATT
ATGCAATTGCCTTTGCTGAAACAGGTCACTTGGTATTTGCAACGCTGCACGCCAACAACGCCAACCAAGCCATCGACCGT
ATTATTCACTTTTTTGAAGCAGACCGTCATAGCCAATTGTTTATGGACTTGTCTTTAAACCTTAAAGCGATGGTTGCTCA
GCAGTTGATTCCAACACCAGACGGTAATTCTCGTCGCGCGGCGATTGAGATTTTGATTAACTCACCGTTAATTTCTGATT
TGATCCGTAAAGGTGAAATCCACGAAATTAAAGACCTGATGAAGCGTTCTCGTGAACTCGGTATGCAAACCTTTGACCAA
GCCTTATTCGATTTGTACAAAGCTAAGCAGATTACCTATAAAGATGCGCTGAAACACGCCGACTCTCCAAACGACTTACG
CTTACAAATCAAGTTGTCTGAAGAAGGCGGTGATCGTCTATTGCATGCCAGTGCAAACATCACGTTTGACGGACAAGCTT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A380UB92

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.984

100

0.78
  pilU Pseudomonas stutzeri DSM 10701

68.464

99.464

0.681
  pilU Vibrio cholerae strain A1552

54.865

99.196

0.544
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.197

92.761

0.391
  pilT Legionella pneumophila strain Lp02

41.124

90.617

0.373
  pilT Legionella pneumophila strain ERS1305867

41.124

90.617

0.373
  pilT Pseudomonas aeruginosa PAK

39.017

92.761

0.362

Multiple sequence alignment