Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GBN67_RS12170 Genome accession   NZ_CP045051
Coordinates   2510751..2511788 (+) Length   345 a.a.
NCBI ID   WP_004694862.1    Uniprot ID   N9CT59
Organism   Acinetobacter johnsonii strain AJ312     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2505751..2516788
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBN67_RS12160 (GBN67_12155) - 2509014..2509748 (-) 735 WP_148826638.1 hypothetical protein -
  GBN67_RS12165 (GBN67_12160) - 2509939..2510628 (-) 690 WP_234962999.1 YggS family pyridoxal phosphate-dependent enzyme -
  GBN67_RS12170 (GBN67_12165) pilT 2510751..2511788 (+) 1038 WP_004694862.1 type IV pilus twitching motility protein PilT Machinery gene
  GBN67_RS12175 (GBN67_12170) pilU 2511815..2512936 (+) 1122 WP_184118209.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GBN67_RS12180 (GBN67_12175) fur 2512996..2513433 (-) 438 WP_004694866.1 ferric iron uptake transcriptional regulator -
  GBN67_RS12185 (GBN67_12180) - 2513546..2513944 (+) 399 WP_004694868.1 outer membrane protein assembly factor BamE -
  GBN67_RS12190 (GBN67_12185) - 2513991..2514314 (-) 324 WP_104222298.1 RnfH family protein -
  GBN67_RS12195 (GBN67_12190) - 2514314..2515396 (-) 1083 WP_234963000.1 hypothetical protein -
  GBN67_RS12200 (GBN67_12195) - 2515539..2515994 (-) 456 WP_005400160.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38292.96 Da        Isoelectric Point: 6.6658

>NTDB_id=391535 GBN67_RS12170 WP_004694862.1 2510751..2511788(+) (pilT) [Acinetobacter johnsonii strain AJ312]
MDITELLAFSAKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKEVHKLVYDIMNDKQRRDFEEKLETDFSFEVPSIAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQTFKELCELPRGLVLVTGPTGSGKSTTLAAMIDYINDNRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KNLVTKGVISPQTARTAAKQPETFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=391535 GBN67_RS12170 WP_004694862.1 2510751..2511788(+) (pilT) [Acinetobacter johnsonii strain AJ312]
ATGGATATCACAGAATTACTAGCCTTTTCGGCTAAGAACGGGGCATCAGATTTACACCTATCTGCAGGTATGCCACCGAT
GATTCGTGTCGATGGTGAAGTTCGTCGTATCAATTTACCAGCTTTAGAACACAAAGAAGTGCATAAACTGGTCTATGACA
TCATGAATGACAAACAACGTCGTGATTTCGAAGAAAAATTAGAAACGGACTTTTCCTTTGAAGTTCCAAGTATTGCTCGT
TTTCGTGTGAACGCATTCAACCAAAACCGCGGTGCAGGTGCAGTATTCCGTACCATTCCTTCTAAAGTCCTGACCATGGA
AGACTTAGGTTTAGGGCAAACCTTTAAAGAGTTGTGTGAATTACCACGTGGTTTAGTTTTAGTGACGGGGCCTACTGGTT
CTGGTAAATCTACCACTTTGGCGGCAATGATCGATTATATTAACGATAACCGCTATGACCATATCTTAACGGTTGAAGAC
CCAATCGAATTTGTACACCAGTCAAAAAAATGCTTGATCAACCAACGTGAAGTGCACCGTGATACACATGGTTTTAACGA
AGCGCTGCGTTCAGCACTACGTGAAGACCCCGATATTATCCTCGTGGGTGAGATGCGTGACCTTGAAACCATTCGTTTGG
CGCTCACAGCAGCAGAAACGGGTCACTTGGTCTTCGGTACACTACATACCACCTCTGCGGCAAAAACCATTGACCGTGTA
ATTGACGTATTCCCTGCTGAAGAAAAAGACATGGTTCGTGCCATGTTGTCTGAATCGCTACAAGCCGTGATTTCTCAAAC
CTTACTCAAGAAAAATGGCGGTGGCCGTGTTGCCGCGCATGAAATCATGATTGGTATTCCTGCCATTCGTAACTTAATTC
GTGAAAACAAAGTAGCACAGATGTATTCTGCTATTCAGACTGGTGCAAACTACGGTATGACCACACTGGATCAAAGTTTG
AAAAACTTGGTCACCAAAGGGGTGATCAGTCCGCAGACCGCACGTACAGCTGCCAAGCAACCTGAAACCTTCTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9CT59

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

94.493

100

0.945
  pilT Acinetobacter baumannii D1279779

94.493

100

0.945
  pilT Acinetobacter baumannii strain A118

94.493

100

0.945
  pilT Acinetobacter baylyi ADP1

86.337

99.71

0.861
  pilT Pseudomonas aeruginosa PAK

84.012

99.71

0.838
  pilT Pseudomonas stutzeri DSM 10701

83.43

99.71

0.832
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Neisseria meningitidis 8013

67.246

100

0.672
  pilT Neisseria gonorrhoeae MS11

66.957

100

0.67
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.452

97.391

0.667
  pilT Vibrio cholerae strain A1552

68.452

97.391

0.667
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.525
  pilU Vibrio cholerae strain A1552

42.183

98.261

0.414
  pilU Pseudomonas stutzeri DSM 10701

42.09

97.101

0.409
  pilU Acinetobacter baylyi ADP1

39.942

99.42

0.397

Multiple sequence alignment