Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GBN67_RS12175 Genome accession   NZ_CP045051
Coordinates   2511815..2512936 (+) Length   373 a.a.
NCBI ID   WP_184118209.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain AJ312     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2506815..2517936
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBN67_RS12160 (GBN67_12155) - 2509014..2509748 (-) 735 WP_148826638.1 hypothetical protein -
  GBN67_RS12165 (GBN67_12160) - 2509939..2510628 (-) 690 WP_234962999.1 YggS family pyridoxal phosphate-dependent enzyme -
  GBN67_RS12170 (GBN67_12165) pilT 2510751..2511788 (+) 1038 WP_004694862.1 type IV pilus twitching motility protein PilT Machinery gene
  GBN67_RS12175 (GBN67_12170) pilU 2511815..2512936 (+) 1122 WP_184118209.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GBN67_RS12180 (GBN67_12175) fur 2512996..2513433 (-) 438 WP_004694866.1 ferric iron uptake transcriptional regulator -
  GBN67_RS12185 (GBN67_12180) - 2513546..2513944 (+) 399 WP_004694868.1 outer membrane protein assembly factor BamE -
  GBN67_RS12190 (GBN67_12185) - 2513991..2514314 (-) 324 WP_104222298.1 RnfH family protein -
  GBN67_RS12195 (GBN67_12190) - 2514314..2515396 (-) 1083 WP_234963000.1 hypothetical protein -
  GBN67_RS12200 (GBN67_12195) - 2515539..2515994 (-) 456 WP_005400160.1 bacteriohemerythrin -
  GBN67_RS12205 (GBN67_12200) - 2516269..2517117 (-) 849 WP_234963001.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41833.12 Da        Isoelectric Point: 6.6664

>NTDB_id=391536 GBN67_RS12175 WP_184118209.1 2511815..2512936(+) (pilU) [Acinetobacter johnsonii strain AJ312]
MDFNDLLNYMIQQKASDLFITADVEPSMKINGQIVPVAKGKLTGDVAGQLLNSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETQIPTMDELKLPPVLKELAMTKRGIIIFVGATGTGKSTSLASIIGYRNQNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHSQLFMDLSLNLKAMVAQQLIPTPDGNSRRAAIEILINSPLISDLIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAKQITYKDALKHADSPNDLRLQIKLSEEGGDRLLHAGANITFDGQA

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=391536 GBN67_RS12175 WP_184118209.1 2511815..2512936(+) (pilU) [Acinetobacter johnsonii strain AJ312]
ATGGATTTTAACGACTTACTCAATTATATGATTCAACAAAAAGCGTCGGATTTATTTATCACCGCTGATGTTGAGCCCTC
CATGAAAATTAACGGTCAAATTGTGCCTGTAGCTAAAGGCAAGCTGACAGGTGATGTCGCGGGGCAACTGCTCAACTCGA
TTATGAGTGAGAAACAGCGCAAAGAATTTGCAGACACACGTGAGTGTAACTTCGCGATTATGAATCGGGATAAAACTGCA
CGTTTTCGTGTCAGTGCCTTTCAACAGCGTGATATGCCAGGTATGGTTTTGCGTCGAATTGAGACGCAAATTCCAACCAT
GGATGAGTTAAAACTCCCGCCTGTGCTCAAAGAGTTGGCCATGACCAAACGTGGCATCATCATCTTTGTCGGTGCAACAG
GTACGGGTAAATCGACCTCTTTGGCTTCGATCATTGGTTATCGTAACCAAAACTCGAAAGGTCATATCATTACCATTGAA
GACCCAATTGAATTTATTCATGAGCATGCAGGCTGTATCATTACGCAGCGTGAAGTGGGCATTGATACGGACTCTTTTGA
AATCGCGCTGAAAAACACCTTACGTCAGGCACCCGATGTGATCCTGATTGGTGAGATTCGTTCCCGTGAAACCATGGATT
ATGCAATTGCCTTCGCGGAAACAGGTCACTTGGTATTTGCAACACTGCACGCCAACAACGCCAACCAAGCCATCGACCGT
ATTATTCACTTCTTTGAAGCAGACCGTCATAGCCAATTGTTTATGGACTTATCTTTAAATCTTAAAGCGATGGTTGCTCA
GCAGTTGATTCCAACACCTGACGGTAATTCACGTCGTGCTGCCATTGAAATTTTGATTAACTCACCGTTAATTTCCGATT
TGATCCGTAAAGGTGAAATCCACGAAATTAAAGACCTGATGAAGCGTTCTCGTGAACTTGGTATGCAAACCTTTGACCAA
GCTTTATTCGATTTGTACAAAGCCAAGCAGATTACCTATAAAGATGCGCTGAAACATGCCGACTCTCCAAACGACTTACG
CTTACAAATCAAGTTGTCTGAAGAAGGTGGTGATCGTTTATTGCATGCCGGTGCAAACATCACGTTTGACGGACAAGCTT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.72

100

0.777
  pilU Pseudomonas stutzeri DSM 10701

67.925

99.464

0.676
  pilU Vibrio cholerae strain A1552

56.98

94.102

0.536
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.486

92.761

0.394
  pilT Legionella pneumophila strain Lp02

41.124

90.617

0.373
  pilT Legionella pneumophila strain ERS1305867

41.124

90.617

0.373
  pilT Pseudomonas aeruginosa PAK

39.017

92.761

0.362

Multiple sequence alignment