Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OCEPR_RS00590 Genome accession   NC_014761
Coordinates   122925..124040 (+) Length   371 a.a.
NCBI ID   WP_013456760.1    Uniprot ID   E4U5N2
Organism   Oceanithermus profundus DSM 14977     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 117925..129040
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCEPR_RS00570 (Ocepr_0123) - 118580..118903 (+) 324 WP_013456756.1 nucleotide pyrophosphohydrolase -
  OCEPR_RS00575 (Ocepr_0124) pgeF 118900..119637 (+) 738 WP_013456757.1 peptidoglycan editing factor PgeF -
  OCEPR_RS00580 (Ocepr_0125) - 119648..120181 (+) 534 WP_013456758.1 YqeG family HAD IIIA-type phosphatase -
  OCEPR_RS00585 (Ocepr_0126) pilF 120262..122913 (+) 2652 WP_013456759.1 type II/IV secretion system protein Machinery gene
  OCEPR_RS00590 (Ocepr_0127) pilT 122925..124040 (+) 1116 WP_013456760.1 type IV pilus twitching motility protein PilT Machinery gene
  OCEPR_RS00595 (Ocepr_0128) - 124134..125630 (+) 1497 WP_013456761.1 ABC transporter ATP-binding protein -
  OCEPR_RS00600 (Ocepr_0129) - 125627..126715 (+) 1089 WP_013456762.1 ABC transporter permease -
  OCEPR_RS00605 (Ocepr_0130) - 126715..127575 (+) 861 WP_013456763.1 ABC transporter permease -
  OCEPR_RS00610 (Ocepr_0131) - 127592..128740 (+) 1149 WP_013456764.1 BMP family ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 40969.02 Da        Isoelectric Point: 6.7818

>NTDB_id=39110 OCEPR_RS00590 WP_013456760.1 122925..124040(+) (pilT) [Oceanithermus profundus DSM 14977]
MQKVPDIVDLLKLSVEREASDLVITVALPPMVKIDGEFHPTEYEPLTPQDTRKLMYALMDEKQQRQFEESKELDFSFSLP
GSGRFRVNVFFQRGSVGGVLRVVPTVIKSFDELGLPKNIGELALSPRGLLLVTGPTGSGKSTTLAAMLDYINENRRAHIV
TIEDPIEFFHKHKMSIINQREVGTDTQGFHEALRSVLRQAPDVILVGEMRDYDTISAAITAAETGHLVMGTLHTNSAPET
IDRIIDVFPQNQQEQVRVQLSNNLVAVLTQQLLPKAFGDGRVLAYELMVATPAVRALIREGKSHQLTSVIQTGGQFGMIT
MDAHLADLYRRKVLSYETALSRAVDPKEFTRLAGVGAGSSVPGGTPTPRRR

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=39110 OCEPR_RS00590 WP_013456760.1 122925..124040(+) (pilT) [Oceanithermus profundus DSM 14977]
ATGCAGAAGGTTCCGGACATCGTCGATCTTCTCAAGCTGTCGGTGGAGCGGGAGGCCTCCGACCTGGTCATCACGGTGGC
GCTGCCGCCGATGGTCAAGATCGACGGCGAGTTCCACCCCACCGAGTACGAGCCGCTCACCCCCCAGGACACCCGCAAGC
TGATGTACGCGCTGATGGACGAGAAGCAGCAGCGGCAGTTCGAGGAGTCGAAGGAGCTCGACTTCTCCTTCAGCCTTCCG
GGCAGCGGCCGCTTCCGCGTCAACGTCTTTTTTCAGCGCGGCTCCGTGGGCGGGGTGTTGCGCGTGGTGCCGACGGTGAT
CAAGTCCTTCGACGAGCTGGGCCTGCCCAAGAACATCGGCGAGCTGGCGCTCAGCCCCCGCGGGCTGCTGCTGGTGACCG
GGCCCACGGGTTCGGGCAAGTCGACCACGCTGGCGGCGATGCTCGACTACATCAACGAGAACCGCCGGGCCCACATCGTC
ACCATCGAGGACCCGATCGAGTTCTTCCACAAGCACAAGATGTCGATCATCAACCAGCGCGAGGTGGGCACGGACACCCA
GGGCTTCCACGAGGCGCTGCGCAGCGTGCTGCGCCAGGCGCCCGACGTGATCCTGGTCGGCGAGATGCGCGACTACGACA
CGATCTCGGCCGCGATCACCGCCGCGGAGACCGGCCACCTGGTCATGGGCACCCTGCACACCAACTCGGCGCCCGAGACG
ATCGACCGCATCATCGACGTCTTCCCGCAGAACCAGCAGGAGCAGGTGCGGGTGCAGCTTTCCAACAACCTGGTGGCCGT
GCTCACCCAGCAGCTGCTGCCCAAGGCCTTCGGCGACGGCCGCGTGCTCGCCTACGAGCTGATGGTGGCCACCCCGGCGG
TGCGGGCGCTGATCCGCGAGGGCAAGAGCCACCAGCTCACCAGCGTGATCCAGACCGGCGGGCAGTTCGGCATGATCACG
ATGGACGCCCACCTGGCCGACCTCTACCGGCGCAAGGTGCTCTCCTACGAGACCGCGCTCTCGCGCGCCGTCGACCCCAA
GGAGTTTACCCGCCTGGCCGGCGTGGGCGCGGGGAGCAGCGTGCCCGGCGGCACCCCGACCCCGCGCCGCCGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(44-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E4U5N2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

63.587

99.191

0.631
  pilT Acinetobacter baumannii D1279779

54.07

92.722

0.501
  pilT Acinetobacter baumannii strain A118

54.07

92.722

0.501
  pilT Acinetobacter nosocomialis M2

54.07

92.722

0.501
  pilT Acinetobacter baylyi ADP1

53.779

92.722

0.499
  pilT Pseudomonas aeruginosa PAK

52.326

92.722

0.485
  pilT Vibrio cholerae strain A1552

52.174

92.992

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.174

92.992

0.485
  pilT Legionella pneumophila strain ERS1305867

51.744

92.722

0.48
  pilT Pseudomonas stutzeri DSM 10701

51.744

92.722

0.48
  pilT Legionella pneumophila strain Lp02

51.744

92.722

0.48
  pilT Neisseria meningitidis 8013

48.547

92.722

0.45
  pilT Neisseria gonorrhoeae MS11

48.256

92.722

0.447
  pilU Pseudomonas stutzeri DSM 10701

45.115

93.801

0.423
  pilU Vibrio cholerae strain A1552

43.103

93.801

0.404
  pilU Acinetobacter baylyi ADP1

42.899

92.992

0.399

Multiple sequence alignment