Detailed information NTG0039109

Bioinformatically predicted

Overview

• Name: pilF
• Type: Machinery gene
• Locus tag: OCEPR_RS00585
• Genome accession: NC_014761
• Coordinates: 120262..122913 (+)
• Length: 883 a.a.
• NCBI ID: WP_013456759.1
• Uniprot ID: E4U5N1
• Organism: Oceanithermus profundus DSM 14977
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 115262..127913

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OCEPR_RS00555 (Ocepr_0120)	-	115815..117011 (-)	1197	WP_013456753.1	CinA family nicotinamide mononucleotide deamidase-related protein	-
OCEPR_RS00560 (Ocepr_0121)	mgsA	117116..117493 (-)	378	WP_013456754.1	methylglyoxal synthase	-
OCEPR_RS00565 (Ocepr_0122)	-	117525..118583 (+)	1059	WP_013456755.1	M42 family metallopeptidase	-
OCEPR_RS00570 (Ocepr_0123)	-	118580..118903 (+)	324	WP_013456756.1	nucleotide pyrophosphohydrolase	-
OCEPR_RS00575 (Ocepr_0124)	pgeF	118900..119637 (+)	738	WP_013456757.1	peptidoglycan editing factor PgeF	-
OCEPR_RS00580 (Ocepr_0125)	-	119648..120181 (+)	534	WP_013456758.1	YqeG family HAD IIIA-type phosphatase	-
OCEPR_RS00585 (Ocepr_0126)	pilF	120262..122913 (+)	2652	WP_013456759.1	type II/IV secretion system protein	Machinery gene
OCEPR_RS00590 (Ocepr_0127)	pilT	122925..124040 (+)	1116	WP_013456760.1	type IV pilus twitching motility protein PilT	Machinery gene
OCEPR_RS00595 (Ocepr_0128)	-	124134..125630 (+)	1497	WP_013456761.1	ABC transporter ATP-binding protein	-
OCEPR_RS00600 (Ocepr_0129)	-	125627..126715 (+)	1089	WP_013456762.1	ABC transporter permease	-
OCEPR_RS00605 (Ocepr_0130)	-	126715..127575 (+)	861	WP_013456763.1	ABC transporter permease	-

Sequence

Protein

• Length: 883 a.a.
• Molecular weight: 98737.16 Da
• Isoelectric Point: 4.7917

>NTDB_id=39109 OCEPR_RS00585 WP_013456759.1 120262..122913(+) (pilF) [Oceanithermus profundus DSM 14977]
MSVLTIGDKRLGAALIELGHLKDEDLQRALERHREIGGSLAEVIVDMGLLSEQRMAQVIEEAFGIPLVNLHEVEIPPEAR
AKLPAEKARELEAIPFALEGDTLRVAFTNPLDTLRVDEVEDLTEMLVEPYQVLRPSFEYALAVNYPELGLETPPPPSPIQ
EHEMRLGELLLQKGLIQREALEQALVEQEQTGELLGRILVGKGLIQEIDLYQALAEQEGAEFLPSTDKVEVDRDTANLFL
RTDALRYHAIPVQQGRKSVRVVVSDLRHKQAVESLLGKRVKFVLTLPEEWEKLFARAYPGSGRLGESLVQDGKLDRDSLR
QALKVQERLGKARPLGEVLVELGYVSAEDVEEALKKQRAGGGRLEDTLVQSGKINPEMLAQSLAIQLGYDYIDPVENPPD
PSVLHLVGENTVRRYTVFPHHLEGDALVVLMKDPRNIWAIDDLRVFTKREIIPAVAAEEQIVKLIERFYGSSGDLDELTK
EFAHKHREEEEVSTDLDDNAVVRLVNTIIREAYLQDASDIHIEPREADVIVRIRIDGALREYMRLPKGAAPAIASRIKIM
GNLDIAERRLPQDGRVRFKDRSIDLDLRLSTLPTVYGEKSVMRLLRKATEIPEIEGLGFAPDVFERFQEVISKPYGIFLI
TGPTGSGKSFTTFSILKRVATPDKNTTTIEDPVEYEIPGINQTQVNMAAGLDFARALRAFLRQDPDIIMVGEIRDSETAQ
IATEAALTGHLVIATLHTNDAAGAITRLDEMGVELFNISASLIGVLAQRLVRKVCDHCKVEVEPDPSVLRRLNLDEKEFK
DVTLYRGVGCERCNGTGYKGRYAIHELMVIDDEIRRAIVEGKSATEIKELARSKGMKTLREDGIYKAMQGITTLEEVMAR
TIE

Nucleotide

• Length: 2652 bp

>NTDB_id=39109 OCEPR_RS00585 WP_013456759.1 120262..122913(+) (pilF) [Oceanithermus profundus DSM 14977]
ATGAGCGTATTGACGATTGGCGATAAGCGGCTCGGCGCCGCACTTATTGAACTCGGCCACTTGAAGGACGAGGATTTGCA
GCGTGCCCTGGAACGGCACCGCGAAATCGGGGGCTCGTTGGCCGAGGTGATCGTGGACATGGGGCTGCTCTCGGAGCAGC
GCATGGCCCAGGTGATCGAAGAAGCCTTCGGGATTCCTCTGGTCAACCTGCACGAGGTGGAGATCCCGCCCGAGGCGCGG
GCCAAGCTGCCCGCGGAAAAGGCGCGGGAGCTCGAGGCCATCCCCTTCGCCCTCGAGGGCGACACCCTGCGCGTCGCCTT
CACCAACCCCCTCGACACCCTGCGCGTCGACGAGGTGGAGGACCTCACCGAGATGCTCGTCGAGCCCTACCAGGTGCTCC
GGCCCTCGTTCGAGTACGCGCTGGCGGTCAACTACCCCGAGCTGGGGCTCGAGACCCCGCCGCCCCCCTCGCCGATCCAG
GAGCACGAGATGCGCCTGGGCGAGCTGCTGTTGCAGAAGGGGCTGATCCAGCGGGAGGCGCTCGAGCAGGCCCTGGTCGA
GCAGGAGCAGACCGGCGAGCTGCTGGGCCGCATCCTCGTCGGCAAGGGGCTGATCCAGGAGATCGACCTCTACCAGGCCC
TGGCCGAGCAGGAAGGGGCCGAGTTCCTACCGAGCACCGACAAGGTCGAGGTCGACCGCGACACCGCCAACCTCTTCCTG
CGCACCGACGCGCTGCGCTACCACGCGATCCCCGTCCAGCAGGGGCGCAAGAGCGTGCGCGTCGTCGTCTCCGACCTGCG
CCACAAGCAGGCCGTCGAGAGCCTGCTGGGCAAGCGGGTGAAGTTCGTCCTCACCCTGCCCGAGGAGTGGGAAAAGCTCT
TCGCCCGCGCCTACCCGGGCTCGGGCCGCCTGGGCGAGTCGCTCGTCCAGGACGGCAAGCTCGACCGCGACAGCCTGCGG
CAGGCGCTCAAGGTCCAGGAGCGCCTGGGCAAGGCGCGGCCGCTGGGCGAGGTTCTCGTCGAGCTCGGCTACGTCTCCGC
CGAGGACGTGGAAGAAGCCCTCAAGAAGCAGCGCGCCGGCGGCGGCCGCCTCGAGGACACCCTGGTCCAGTCGGGCAAGA
TCAACCCCGAGATGCTGGCCCAGAGCCTGGCCATCCAGCTGGGCTACGACTACATTGACCCCGTGGAGAACCCGCCCGAC
CCCAGCGTGCTGCACCTGGTGGGCGAGAATACGGTGCGCCGCTACACGGTCTTCCCGCACCACCTCGAAGGCGACGCCCT
GGTGGTGCTGATGAAGGACCCGCGCAACATCTGGGCGATCGACGACCTGCGGGTCTTCACCAAGCGCGAGATCATCCCCG
CGGTGGCCGCCGAGGAGCAGATCGTCAAGCTGATCGAGCGCTTCTACGGCTCGAGCGGCGACCTCGACGAGCTGACCAAG
GAGTTCGCCCACAAGCACCGCGAGGAAGAGGAGGTCTCCACCGACCTCGACGACAACGCGGTGGTGCGGCTCGTCAACAC
CATCATCCGCGAGGCCTACCTGCAGGACGCCTCGGACATCCACATCGAGCCGCGCGAGGCCGACGTGATCGTGCGCATCC
GCATCGACGGCGCGCTGCGCGAGTACATGCGCCTGCCCAAGGGCGCGGCCCCGGCCATCGCCAGCCGCATCAAGATCATG
GGCAACCTCGACATCGCCGAGCGCCGCCTGCCCCAGGACGGCCGCGTGCGCTTCAAGGACCGCAGCATCGACCTCGACCT
GCGCCTTTCCACGCTGCCCACGGTCTACGGCGAGAAGTCGGTGATGCGCCTCTTGCGCAAGGCCACCGAGATCCCCGAGA
TCGAGGGATTGGGCTTCGCCCCCGACGTCTTCGAGCGCTTCCAGGAGGTCATCAGCAAGCCCTACGGCATCTTCCTGATC
ACCGGCCCCACCGGTTCGGGTAAGTCGTTCACTACCTTCTCGATCCTCAAGCGGGTGGCCACCCCCGACAAGAACACGAC
CACGATCGAGGACCCGGTGGAGTACGAGATCCCCGGCATCAACCAGACCCAGGTGAACATGGCCGCGGGCCTCGACTTCG
CCCGCGCCCTGCGCGCCTTCCTGCGCCAGGATCCGGACATCATCATGGTCGGTGAGATCCGCGACTCGGAGACCGCGCAG
ATCGCCACCGAGGCCGCGCTCACCGGCCACCTGGTCATCGCCACCCTGCACACCAACGACGCCGCCGGCGCGATCACCCG
CCTCGACGAGATGGGCGTCGAGCTCTTCAACATCTCGGCCTCGCTGATCGGCGTGCTGGCCCAGCGCCTGGTGCGCAAGG
TCTGCGACCACTGCAAGGTCGAGGTCGAGCCCGACCCCTCGGTGCTGCGGCGCCTCAACCTCGACGAGAAGGAGTTCAAG
GACGTCACCCTCTACCGCGGCGTCGGCTGCGAGCGCTGCAACGGCACCGGCTACAAGGGGCGCTACGCCATCCACGAACT
GATGGTCATCGACGACGAGATCCGCCGGGCCATCGTCGAGGGCAAGTCGGCCACCGAGATCAAGGAACTCGCACGCAGCA
AGGGGATGAAGACGCTGCGCGAGGACGGCATCTACAAGGCCATGCAGGGCATCACCACGCTGGAAGAGGTCATGGCCCGC
ACGATCGAGTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (368-473)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (45-142)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (199-301)

• Type II/IV secretion system protein (T2SSE) (507-773)

3D structure

• AlphaFold DB: E4U5N1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilF	Thermus thermophilus HB27	71.268	100	0.719
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	57.579	100	0.576