Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   F8A90_RS00880 Genome accession   NZ_CP044522
Coordinates   189479..190510 (+) Length   343 a.a.
NCBI ID   WP_166019003.1    Uniprot ID   -
Organism   Cobetia sp. cqz5-12     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 184479..195510
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F8A90_RS00855 (F8A90_00855) metW 184782..185396 (-) 615 WP_200018447.1 methionine biosynthesis protein MetW -
  F8A90_RS00860 (F8A90_00860) - 185389..186585 (-) 1197 WP_200018448.1 homoserine O-acetyltransferase -
  F8A90_RS00865 (F8A90_00865) - 186869..187450 (-) 582 WP_043333437.1 YggT family protein -
  F8A90_RS00870 (F8A90_00870) proC 187600..188442 (-) 843 WP_200018449.1 pyrroline-5-carboxylate reductase -
  F8A90_RS00875 (F8A90_00875) - 188531..189283 (-) 753 WP_200018455.1 YggS family pyridoxal phosphate-dependent enzyme -
  F8A90_RS00880 (F8A90_00880) pilT 189479..190510 (+) 1032 WP_166019003.1 type IV pilus twitching motility protein PilT Machinery gene
  F8A90_RS00885 (F8A90_00885) pilU 190650..191804 (+) 1155 WP_166019002.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  F8A90_RS00890 (F8A90_00890) - 191911..193059 (-) 1149 WP_200018457.1 anhydro-N-acetylmuramic acid kinase -
  F8A90_RS00895 (F8A90_00895) tyrS 193215..194423 (+) 1209 WP_200018459.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 343 a.a.        Molecular weight: 37698.31 Da        Isoelectric Point: 6.0550

>NTDB_id=390815 F8A90_RS00880 WP_166019003.1 189479..190510(+) (pilT) [Cobetia sp. cqz5-12]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDVRRINVPSMDDKQVRALIYEIMNDKQRRDFEEFLETDFSFEVPGVSR
FRVNAFHQGRGAGAVFRTIPSEVLTMEDLGLGEAFRKMCMLPRGLILVTGPTGSGKSTTLAAMVDYINENKYDHILTIED
PVEFVHQSKRCLVNQREVHRDTHGFAPALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSATKTVDRI
IDVFPGEEKSMVRSMLSESLQGVIAQTLLKKVGGGRVAAHEIMIATPAIRNLIREDKVAQMYSSIQTGGALGMQTLDAAL
TRLLAEGLVTNEDAQAKAKGLLS

Nucleotide


Download         Length: 1032 bp        

>NTDB_id=390815 F8A90_RS00880 WP_166019003.1 189479..190510(+) (pilT) [Cobetia sp. cqz5-12]
ATGGATATTACCGAACTGCTGGCTTTTTCGGCAAAACAGAACGCGTCGGATCTTCACCTGTCCGCAGGTTTGCCACCGAT
GATTCGTGTCGACGGTGATGTGCGGCGCATCAATGTGCCGTCAATGGATGACAAGCAGGTGCGCGCGCTGATCTACGAGA
TCATGAACGACAAGCAGCGCCGTGACTTCGAGGAGTTCCTCGAGACCGATTTCTCCTTCGAGGTGCCGGGCGTCTCGCGC
TTTCGTGTCAACGCCTTCCATCAGGGGCGTGGCGCGGGGGCCGTCTTCCGTACCATCCCCTCGGAAGTGCTGACGATGGA
GGATCTGGGGCTCGGTGAAGCCTTCCGCAAGATGTGCATGCTGCCGCGTGGCCTGATTCTTGTCACCGGACCGACTGGCT
CCGGTAAATCCACCACGCTGGCGGCGATGGTCGATTACATCAACGAGAACAAGTACGACCACATCCTCACCATCGAGGAC
CCGGTGGAATTCGTGCACCAGTCCAAGCGCTGTCTGGTCAACCAGCGTGAAGTGCACCGTGACACCCACGGCTTCGCGCC
CGCGCTGCGTTCGGCGCTGCGTGAAGACCCGGATGTCATTCTGGTCGGCGAGCTGCGGGATCTCGAGACCATCCGTCTGG
CACTGACCGCGGCCGAGACGGGCCACCTGGTGTTCGGCACCCTGCACACCACCTCCGCGACCAAGACCGTCGACCGTATC
ATCGATGTCTTCCCCGGCGAGGAGAAGTCGATGGTGCGCTCGATGCTGTCCGAGTCCTTGCAGGGTGTCATCGCCCAGAC
ACTGCTCAAGAAAGTTGGCGGTGGCAGGGTGGCGGCTCACGAGATCATGATCGCGACGCCTGCCATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCCATCCAGACCGGTGGCGCCCTGGGCATGCAGACATTGGATGCAGCGCTG
ACGCGTCTGCTGGCGGAAGGGCTGGTGACCAATGAAGATGCTCAGGCGAAAGCCAAGGGCTTGTTGAGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

84.366

98.834

0.834
  pilT Pseudomonas aeruginosa PAK

82.006

98.834

0.81
  pilT Acinetobacter baumannii D1279779

78.466

98.834

0.776
  pilT Acinetobacter baumannii strain A118

78.466

98.834

0.776
  pilT Acinetobacter nosocomialis M2

78.171

98.834

0.773
  pilT Acinetobacter baylyi ADP1

77.876

98.834

0.77
  pilT Legionella pneumophila strain Lp02

72.781

98.542

0.717
  pilT Legionella pneumophila strain ERS1305867

72.781

98.542

0.717
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.623

98.251

0.694
  pilT Vibrio cholerae strain A1552

70.623

98.251

0.694
  pilT Neisseria meningitidis 8013

65.689

99.417

0.653
  pilT Neisseria gonorrhoeae MS11

65.396

99.417

0.65
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.327

98.834

0.507
  pilU Pseudomonas stutzeri DSM 10701

42.985

97.668

0.42
  pilU Vibrio cholerae strain A1552

41.194

97.668

0.402
  pilU Acinetobacter baylyi ADP1

39.412

99.125

0.391

Multiple sequence alignment