Detailed information NTG0390816

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: F8A90_RS00885
• Genome accession: NZ_CP044522
• Coordinates: 190650..191804 (+)
• Length: 384 a.a.
• NCBI ID: WP_166019002.1
• Uniprot ID: -
• Organism: Cobetia sp. cqz5-12
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 185650..196804

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
F8A90_RS00865 (F8A90_00865)	-	186869..187450 (-)	582	WP_043333437.1	YggT family protein	-
F8A90_RS00870 (F8A90_00870)	proC	187600..188442 (-)	843	WP_200018449.1	pyrroline-5-carboxylate reductase	-
F8A90_RS00875 (F8A90_00875)	-	188531..189283 (-)	753	WP_200018455.1	YggS family pyridoxal phosphate-dependent enzyme	-
F8A90_RS00880 (F8A90_00880)	pilT	189479..190510 (+)	1032	WP_166019003.1	type IV pilus twitching motility protein PilT	Machinery gene
F8A90_RS00885 (F8A90_00885)	pilU	190650..191804 (+)	1155	WP_166019002.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
F8A90_RS00890 (F8A90_00890)	-	191911..193059 (-)	1149	WP_200018457.1	anhydro-N-acetylmuramic acid kinase	-
F8A90_RS00895 (F8A90_00895)	tyrS	193215..194423 (+)	1209	WP_200018459.1	tyrosine--tRNA ligase	-

Sequence

Protein

• Length: 384 a.a.
• Molecular weight: 43052.33 Da
• Isoelectric Point: 6.3429

>NTDB_id=390816 F8A90_RS00885 WP_166019002.1 190650..191804(+) (pilU) [Cobetia sp. cqz5-12]
MTPHEWLHELLQLMVSKGSSDLFISTGTPPQMKVNGRMVALGDKKLGVDQVRELVLAPMSDMQRERFEEEREANFAHSLP
GVGRFRISAFYQRSQMGMVIRRIQLSIPSLEELRLPEIIKGLSETKRGLVIFVGGTGAGKSTSLAAMIQHRNQTSSGHII
CIEDPIEYIHPHQRSIVTQREVGIDTESFEVALRNTLRQAPDVIMIGEIRSRETMEHALTFAETGHLCLATLHANNANQA
LDRIIHFFPEERHEQVWMDLSLNLKGIVAQQLLPHRSGNTTQRVPAIEVMLRSPLIVDLIRKGAVVEIKDVMKRSQQQGM
MTFDQSLYALHQQGLITEEVALAHADSANDLRLMIKFGDSDSAQEAQLDVLNAASRFSLQGDDD

Nucleotide

• Length: 1155 bp

>NTDB_id=390816 F8A90_RS00885 WP_166019002.1 190650..191804(+) (pilU) [Cobetia sp. cqz5-12]
ATGACTCCTCATGAATGGCTGCATGAACTTCTGCAGTTGATGGTCAGCAAGGGCAGCTCGGACCTGTTCATCTCCACCGG
CACGCCGCCGCAGATGAAGGTCAATGGCCGCATGGTGGCGCTGGGTGACAAGAAGCTGGGTGTCGATCAGGTGAGAGAGC
TGGTGCTCGCGCCGATGAGTGACATGCAGCGTGAGCGCTTCGAGGAAGAGCGCGAGGCCAACTTCGCGCATAGTCTGCCG
GGAGTCGGGCGCTTCAGGATCAGTGCCTTCTACCAGCGTAGCCAGATGGGCATGGTGATTCGCCGTATCCAGCTGTCAAT
TCCCAGCCTGGAAGAGTTGCGGCTACCGGAGATCATCAAGGGGCTTTCCGAGACCAAGCGTGGTCTGGTGATCTTCGTCG
GCGGTACCGGCGCCGGCAAGTCGACCTCGCTGGCAGCGATGATCCAGCACCGCAACCAGACCTCGAGCGGGCATATCATC
TGTATCGAAGACCCGATCGAGTATATCCACCCTCACCAGCGCTCCATCGTGACGCAGCGTGAAGTGGGGATCGATACCGA
GTCCTTCGAGGTGGCACTGCGCAATACGCTGCGTCAGGCGCCGGACGTGATCATGATCGGTGAGATCCGCTCGCGTGAGA
CCATGGAACACGCGCTGACCTTCGCCGAGACAGGCCACCTGTGTCTTGCGACGTTGCATGCCAACAATGCCAACCAGGCC
CTGGATCGCATCATCCACTTCTTCCCGGAGGAGCGTCATGAGCAGGTGTGGATGGATCTATCGCTGAACCTCAAGGGCAT
CGTGGCCCAGCAGCTGTTGCCTCACAGGAGTGGCAATACCACCCAGCGCGTGCCGGCCATTGAGGTGATGCTGCGTTCGC
CGCTGATCGTCGACCTGATCCGCAAGGGCGCGGTGGTCGAGATCAAGGACGTCATGAAGCGCTCGCAGCAGCAGGGCATG
ATGACATTTGATCAATCGTTGTATGCCTTGCATCAGCAGGGCTTGATCACTGAGGAGGTGGCATTGGCGCATGCCGATTC
CGCCAATGACCTGCGCCTGATGATCAAGTTCGGCGATTCCGATAGCGCCCAGGAAGCCCAGCTGGATGTCCTGAATGCCG
CCAGTCGCTTCTCGCTGCAGGGGGATGACGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	65.341	91.667	0.599
pilU	Vibrio cholerae strain A1552	56.369	96.094	0.542
pilU	Acinetobacter baylyi ADP1	55.932	92.188	0.516
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	44.058	89.844	0.396
pilT	Pseudomonas aeruginosa PAK	41.742	86.719	0.362
pilT	Neisseria meningitidis 8013	40.525	89.323	0.362