Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FSC10_RS11655 Genome accession   NZ_CP044463
Coordinates   2424682..2425719 (+) Length   345 a.a.
NCBI ID   WP_004811122.1    Uniprot ID   A0A1P8PHZ9
Organism   Acinetobacter schindleri strain HZE23-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2419682..2430719
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FSC10_RS11645 (FSC10_11675) - 2422720..2423826 (+) 1107 WP_163171948.1 acyltransferase family protein -
  FSC10_RS11650 (FSC10_11680) - 2423872..2424558 (-) 687 WP_163172755.1 YggS family pyridoxal phosphate-dependent enzyme -
  FSC10_RS11655 (FSC10_11685) pilT 2424682..2425719 (+) 1038 WP_004811122.1 type IV pilus twitching motility protein PilT Machinery gene
  FSC10_RS11660 (FSC10_11690) pilU 2425752..2426873 (+) 1122 WP_048881346.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FSC10_RS11665 (FSC10_11695) fur 2426952..2427389 (-) 438 WP_004811125.1 ferric iron uptake transcriptional regulator -
  FSC10_RS11670 (FSC10_11700) - 2427502..2427900 (+) 399 WP_004811126.1 outer membrane protein assembly factor BamE -
  FSC10_RS11675 (FSC10_11705) - 2428010..2428336 (-) 327 WP_163171950.1 RnfH family protein -
  FSC10_RS11680 (FSC10_11710) - 2428336..2429415 (-) 1080 WP_163171952.1 hypothetical protein -
  FSC10_RS11685 (FSC10_11715) - 2429555..2430010 (-) 456 WP_004893331.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38209.77 Da        Isoelectric Point: 6.5054

>NTDB_id=390176 FSC10_RS11655 WP_004811122.1 2424682..2425719(+) (pilT) [Acinetobacter schindleri strain HZE23-1]
MDITELLAFSAKNGASDLHLSAGMPPLIRVDGEVRRINLPALEHKEVHKLVYDIMNDKQRRDYEENLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTIEDLGMGQIFKDICDYPRGLVLVTGPTGSGKSTTLAAMLDYINDNRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVVSQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KTLVSKGVISPQVARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=390176 FSC10_RS11655 WP_004811122.1 2424682..2425719(+) (pilT) [Acinetobacter schindleri strain HZE23-1]
ATGGACATTACAGAATTATTAGCATTTTCTGCTAAAAATGGCGCGTCAGATTTACACTTGTCTGCGGGGATGCCACCACT
GATTCGTGTTGATGGTGAAGTTCGTCGTATTAACCTGCCTGCCCTGGAACATAAAGAAGTGCACAAGCTGGTTTACGATA
TCATGAACGATAAACAGCGCCGTGACTATGAAGAAAATCTAGAAACTGACTTTTCCTTTGAAGTTCCTGGCGTTGCCCGT
TTCCGTGTGAACGCATTTAACCAGAACCGGGGTGCCGGTGCCGTATTCCGTACCATTCCCTCTAAAGTTTTAACCATTGA
AGATCTGGGCATGGGTCAGATCTTTAAAGATATTTGTGACTATCCACGTGGTCTGGTACTGGTCACAGGCCCAACCGGTT
CAGGTAAATCAACGACGCTGGCAGCGATGCTAGACTATATCAATGATAACCGTTATGACCATATTCTGACCGTCGAAGAT
CCAATTGAATTTGTACACCAATCCAAAAAATGTCTGATCAACCAGCGTGAAGTGCATCGTGACACGCATGGTTTCAATGA
AGCATTACGTTCTGCATTACGTGAAGACCCGGATATTATCCTGGTGGGTGAGATGCGTGACCTTGAAACCATCCGTCTGG
CACTCACTGCTGCTGAAACCGGTCACCTGGTGTTTGGCACCCTGCATACGACTTCTGCTGCTAAAACTATTGACCGTGTG
ATCGACGTATTCCCGGCCGAAGAAAAAGACATGGTTCGTGCCATGCTGTCTGAATCATTACAAGCGGTTGTCTCACAAAC
CCTGCTCAAGAAAAATGGTGGTGGTCGTGTCGCTGCACATGAAATCATGATCGGTATTCCGGCCATCCGTAACCTGATCC
GCGAAAACAAAGTTGCTCAAATGTACTCTGCCATTCAGACCGGTGCCAACTATGGTATGACTACGCTGGATCAGAGCCTG
AAAACACTGGTATCTAAAGGCGTGATCAGTCCACAAGTTGCGCGTACTGCTGCTAAACAGCCTGAATCTTTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1P8PHZ9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

95.652

100

0.957
  pilT Acinetobacter baumannii D1279779

95.362

100

0.954
  pilT Acinetobacter baumannii strain A118

95.362

100

0.954
  pilT Acinetobacter baylyi ADP1

87.209

99.71

0.87
  pilT Pseudomonas aeruginosa PAK

84.302

99.71

0.841
  pilT Pseudomonas stutzeri DSM 10701

84.012

99.71

0.838
  pilT Legionella pneumophila strain Lp02

74.709

99.71

0.745
  pilT Legionella pneumophila strain ERS1305867

74.709

99.71

0.745
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.536

100

0.675
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.88

96.232

0.672
  pilT Vibrio cholerae strain A1552

69.88

96.232

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.519
  pilU Vibrio cholerae strain A1552

41.298

98.261

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.692

95.942

0.4
  pilU Acinetobacter baylyi ADP1

38.15

100

0.383

Multiple sequence alignment