Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MPQ_RS03160 Genome accession   NC_014733
Coordinates   647491..648534 (+) Length   347 a.a.
NCBI ID   WP_013441454.1    Uniprot ID   -
Organism   Methylovorus sp. MP688     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 642491..653534
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MPQ_RS03130 (MPQ_0643) - 642558..643106 (+) 549 WP_013441448.1 DJ-1 family glyoxalase III -
  MPQ_RS03135 (MPQ_0644) - 643103..643693 (+) 591 WP_041361989.1 nucleoside triphosphate pyrophosphatase -
  MPQ_RS03140 (MPQ_0645) rng 643725..645182 (+) 1458 WP_013441450.1 ribonuclease G -
  MPQ_RS03145 (MPQ_0646) - 645281..645856 (-) 576 WP_013441451.1 YggT family protein -
  MPQ_RS03150 (MPQ_0647) proC 645858..646670 (-) 813 WP_013441452.1 pyrroline-5-carboxylate reductase -
  MPQ_RS03155 (MPQ_0648) - 646747..647448 (-) 702 WP_013441453.1 YggS family pyridoxal phosphate-dependent enzyme -
  MPQ_RS03160 (MPQ_0649) pilT 647491..648534 (+) 1044 WP_013441454.1 type IV pilus twitching motility protein PilT Machinery gene
  MPQ_RS03165 (MPQ_0650) pilU 648583..649719 (+) 1137 WP_013441455.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MPQ_RS03170 (MPQ_0651) - 649795..650763 (+) 969 WP_013441456.1 DUF72 domain-containing protein -
  MPQ_RS03175 (MPQ_0652) - 650823..652100 (-) 1278 WP_013441457.1 dihydroorotase -
  MPQ_RS03180 (MPQ_0653) - 652097..653050 (-) 954 WP_013441458.1 aspartate carbamoyltransferase catalytic subunit -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38356.94 Da        Isoelectric Point: 6.5020

>NTDB_id=38996 MPQ_RS03160 WP_013441454.1 647491..648534(+) (pilT) [Methylovorus sp. MP688]
MDISDLLAFSVKNKASDLHLSAGMPPMIRVHGDVRKINLPSMDHSQVHDMVYDIMNDGQRKIYEETLECDFSFEIPNLAR
FRVNAFNQNRGAGAVFRTIPSKILSMEELNCPPIFREIAQNPRGIVLVTGPTGSGKSTTLAAMVDFINENEYGHILTVED
PIEFVHTSKKCLINQREVGPHTLSFSNALRSALREDPDVILVGEMRDLETIRLALSAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKEMVRSMLSESLRAVISQTLCKTKDEQGRVAAHEIMIGTPAIRNLIRENKIAQMYSAIQTGQNVGMQTLDQN
LQELVKRNIISVGEARGKAANKESFGG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=38996 MPQ_RS03160 WP_013441454.1 647491..648534(+) (pilT) [Methylovorus sp. MP688]
GTGGATATTTCGGATTTGCTGGCGTTCTCGGTTAAAAACAAGGCTTCTGATTTGCATCTTTCCGCCGGTATGCCACCCAT
GATCCGGGTGCATGGCGACGTGCGCAAAATCAACCTGCCCTCCATGGATCACAGCCAGGTGCACGACATGGTGTATGACA
TCATGAACGACGGCCAGCGCAAGATTTACGAAGAAACCCTGGAGTGCGATTTCTCGTTCGAGATTCCCAATCTTGCCCGT
TTCCGGGTCAATGCCTTCAATCAGAATCGCGGTGCGGGCGCGGTATTCCGTACCATTCCTTCCAAGATTCTGAGCATGGA
AGAGCTGAACTGTCCGCCTATCTTCCGTGAAATCGCGCAAAACCCGCGCGGCATCGTGCTGGTGACCGGGCCGACCGGTT
CCGGCAAATCCACTACGCTGGCGGCCATGGTCGATTTCATCAATGAAAACGAGTACGGCCATATCCTGACGGTGGAAGAC
CCGATTGAGTTCGTCCACACCTCGAAAAAGTGTTTGATCAACCAGCGCGAAGTCGGTCCGCATACCCTGTCGTTCTCGAA
TGCCTTGCGCTCAGCGCTGCGTGAAGACCCTGACGTGATTCTGGTTGGCGAAATGCGCGACCTGGAAACCATACGCCTGG
CCCTGTCGGCCGCTGAAACCGGCCACCTGGTGTTTGGCACCCTGCATACCAGCTCGGCGGCCAAGACCATAGACCGTATC
GTCGATGTATTCCCCGCGGCCGAAAAGGAAATGGTGCGCTCCATGCTCTCTGAGTCCTTACGGGCGGTAATCTCGCAAAC
ACTGTGCAAAACCAAGGATGAGCAGGGCCGTGTTGCCGCGCATGAGATCATGATAGGCACGCCGGCCATCCGTAACCTCA
TCCGCGAGAACAAGATTGCGCAGATGTATTCCGCCATCCAGACCGGGCAAAATGTGGGCATGCAGACGCTGGACCAGAAC
CTGCAGGAGCTGGTGAAGCGCAATATCATTTCGGTGGGCGAAGCCCGAGGCAAGGCTGCCAACAAGGAATCCTTCGGCGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.681

99.424

0.772
  pilT Acinetobacter baumannii D1279779

77.681

99.424

0.772
  pilT Acinetobacter baumannii strain A118

77.681

99.424

0.772
  pilT Acinetobacter baylyi ADP1

75.652

99.424

0.752
  pilT Pseudomonas stutzeri DSM 10701

75.072

99.424

0.746
  pilT Pseudomonas aeruginosa PAK

75.072

99.424

0.746
  pilT Legionella pneumophila strain Lp02

74.783

99.424

0.744
  pilT Legionella pneumophila strain ERS1305867

74.783

99.424

0.744
  pilT Neisseria meningitidis 8013

72.174

99.424

0.718
  pilT Neisseria gonorrhoeae MS11

71.884

99.424

0.715
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.751

97.406

0.66
  pilT Vibrio cholerae strain A1552

67.751

97.406

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

99.424

0.504
  pilU Acinetobacter baylyi ADP1

42.857

96.83

0.415
  pilU Pseudomonas stutzeri DSM 10701

40.896

96.542

0.395
  pilU Vibrio cholerae strain A1552

40.597

96.542

0.392

Multiple sequence alignment