Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MPQ_RS03165 Genome accession   NC_014733
Coordinates   648583..649719 (+) Length   378 a.a.
NCBI ID   WP_013441455.1    Uniprot ID   -
Organism   Methylovorus sp. MP688     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 643583..654719
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MPQ_RS03140 (MPQ_0645) rng 643725..645182 (+) 1458 WP_013441450.1 ribonuclease G -
  MPQ_RS03145 (MPQ_0646) - 645281..645856 (-) 576 WP_013441451.1 YggT family protein -
  MPQ_RS03150 (MPQ_0647) proC 645858..646670 (-) 813 WP_013441452.1 pyrroline-5-carboxylate reductase -
  MPQ_RS03155 (MPQ_0648) - 646747..647448 (-) 702 WP_013441453.1 YggS family pyridoxal phosphate-dependent enzyme -
  MPQ_RS03160 (MPQ_0649) pilT 647491..648534 (+) 1044 WP_013441454.1 type IV pilus twitching motility protein PilT Machinery gene
  MPQ_RS03165 (MPQ_0650) pilU 648583..649719 (+) 1137 WP_013441455.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MPQ_RS03170 (MPQ_0651) - 649795..650763 (+) 969 WP_013441456.1 DUF72 domain-containing protein -
  MPQ_RS03175 (MPQ_0652) - 650823..652100 (-) 1278 WP_013441457.1 dihydroorotase -
  MPQ_RS03180 (MPQ_0653) - 652097..653050 (-) 954 WP_013441458.1 aspartate carbamoyltransferase catalytic subunit -
  MPQ_RS03185 (MPQ_0654) pyrR 653050..653547 (-) 498 WP_013441459.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  MPQ_RS03190 (MPQ_0655) ruvX 653571..654089 (-) 519 WP_013441460.1 Holliday junction resolvase RuvX -
  MPQ_RS03195 (MPQ_0656) - 654122..654682 (-) 561 WP_013441461.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42518.07 Da        Isoelectric Point: 6.1875

>NTDB_id=38997 MPQ_RS03165 WP_013441455.1 648583..649719(+) (pilU) [Methylovorus sp. MP688]
MEEGQAEKFVFDLLRMMIAKKASDLFITAAFPPAMKIDGKMTPMSSQPLTPQHVREIARAIMNDKQAAEFEATRECNFAI
SMPRVARFRVNAFVQRGSVGLVFRTITSAIPKFDDLRLPEVLKEVSMTKRGLVILVGGTGSGKSTTLAAMMGYRNENSYG
HIITIEDPIEFIHDHKNCIVTQREVGVDTDNWFAALKNTLRQAPDVIMIGEIRDRETMDYAIAFAETGHLCMATLHANST
NQALDRIINFFPEERRQQLLMDLSLNMRAFVSQRLIPKRDGQGRAAAIEIMLNSPLISDLVLKGEVHGIKEIMAKSRELG
MQTFDQALFELYERDEISYEDALRNADSINDIRLKIKLEGKDAQDRDMSSGLDKLGIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=38997 MPQ_RS03165 WP_013441455.1 648583..649719(+) (pilU) [Methylovorus sp. MP688]
ATGGAAGAAGGTCAGGCAGAGAAGTTTGTTTTTGATTTGTTGCGCATGATGATTGCCAAGAAAGCCTCTGATCTGTTCAT
CACGGCGGCCTTTCCACCGGCAATGAAGATCGATGGCAAGATGACGCCCATGAGCAGCCAGCCACTCACGCCCCAGCACG
TGCGCGAGATTGCCCGTGCCATCATGAACGACAAGCAGGCTGCCGAATTCGAAGCGACCCGCGAATGCAATTTCGCCATC
AGCATGCCGCGTGTGGCCCGGTTCCGGGTCAATGCCTTTGTGCAGCGTGGCTCGGTCGGTCTGGTATTCCGGACCATTAC
CTCGGCTATTCCAAAGTTTGATGACCTGCGCCTGCCTGAGGTATTGAAGGAAGTCTCCATGACCAAGCGCGGCCTGGTGA
TTCTGGTCGGCGGCACGGGCTCCGGCAAATCCACCACGCTGGCGGCGATGATGGGCTACCGCAACGAAAACAGTTACGGT
CATATCATCACCATTGAAGACCCGATTGAATTCATACACGACCACAAAAACTGCATCGTGACCCAGCGCGAAGTGGGCGT
GGATACCGATAACTGGTTTGCTGCGCTGAAAAACACCCTGCGCCAGGCGCCTGATGTGATCATGATCGGCGAAATCCGCG
ATCGCGAAACCATGGACTACGCCATTGCCTTTGCCGAAACCGGCCACCTGTGCATGGCAACACTGCACGCCAACAGCACC
AACCAGGCGCTGGACCGCATTATCAACTTCTTCCCTGAAGAGCGTCGTCAGCAATTGTTGATGGATCTGTCGCTGAACAT
GCGGGCCTTTGTCTCGCAACGCCTGATTCCGAAGCGTGATGGTCAGGGCCGTGCGGCGGCGATTGAGATCATGCTGAATT
CCCCCCTTATTTCTGACCTGGTGCTGAAGGGCGAAGTGCATGGCATCAAGGAGATCATGGCGAAGTCACGCGAACTGGGC
ATGCAGACCTTTGACCAGGCCCTGTTTGAGTTGTATGAGCGCGATGAAATCAGCTACGAAGATGCACTGCGCAATGCAGA
CTCCATCAACGATATCCGCCTCAAGATCAAGCTGGAGGGCAAGGACGCGCAAGATCGCGATATGTCCTCCGGGCTCGATA
AACTGGGAATAGTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.732

94.709

0.585
  pilU Acinetobacter baylyi ADP1

60.057

92.063

0.553
  pilU Vibrio cholerae strain A1552

54.648

93.915

0.513
  pilT Pseudomonas aeruginosa PAK

44.214

89.153

0.394
  pilT Legionella pneumophila strain ERS1305867

43.844

88.095

0.386
  pilT Legionella pneumophila strain Lp02

43.844

88.095

0.386
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.323

89.153

0.386
  pilT Acinetobacter nosocomialis M2

42.943

88.095

0.378
  pilT Acinetobacter baumannii D1279779

42.943

88.095

0.378
  pilT Acinetobacter baumannii strain A118

42.943

88.095

0.378
  pilT Pseudomonas stutzeri DSM 10701

42.433

89.153

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.462

85.979

0.365
  pilT Vibrio cholerae strain A1552

42.462

85.979

0.365
  pilT Acinetobacter baylyi ADP1

41.141

88.095

0.362

Multiple sequence alignment