Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FOB71_RS21375 Genome accession   NZ_CP044069
Coordinates   2893259..2894296 (-) Length   345 a.a.
NCBI ID   WP_026050359.1    Uniprot ID   -
Organism   Vibrio vulnificus strain FDAARGOS_663     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2888259..2899296
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOB71_RS21350 (FOB71_21345) rsmE 2889280..2890011 (+) 732 WP_017428735.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  FOB71_RS21355 (FOB71_21350) gshB 2890026..2890979 (+) 954 WP_026050357.1 glutathione synthase -
  FOB71_RS21360 (FOB71_21355) - 2891034..2891597 (+) 564 WP_011079465.1 YqgE/AlgH family protein -
  FOB71_RS21365 (FOB71_21360) ruvX 2891619..2892041 (+) 423 WP_011079464.1 Holliday junction resolvase RuvX -
  FOB71_RS21370 (FOB71_21365) pilU 2892134..2893240 (-) 1107 WP_026050358.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FOB71_RS21375 (FOB71_21370) pilT 2893259..2894296 (-) 1038 WP_026050359.1 type IV pilus twitching motility protein PilT Machinery gene
  FOB71_RS21380 (FOB71_21375) - 2894321..2895031 (+) 711 WP_017428739.1 YggS family pyridoxal phosphate-dependent enzyme -
  FOB71_RS21385 (FOB71_21380) proC 2895066..2895884 (+) 819 WP_017428740.1 pyrroline-5-carboxylate reductase -
  FOB71_RS21390 (FOB71_21385) - 2895926..2896483 (+) 558 WP_013571095.1 YggT family protein -
  FOB71_RS21395 (FOB71_21390) yggU 2896483..2896773 (+) 291 WP_017428741.1 DUF167 family protein YggU -
  FOB71_RS21400 (FOB71_21395) - 2896816..2897247 (+) 432 WP_017428742.1 DUF4426 domain-containing protein -
  FOB71_RS21405 (FOB71_21400) - 2897258..2897860 (+) 603 WP_026050360.1 XTP/dITP diphosphatase -
  FOB71_RS21410 (FOB71_21405) hemW 2897870..2899042 (+) 1173 WP_017428744.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38533.19 Da        Isoelectric Point: 6.3696

>NTDB_id=387395 FOB71_RS21375 WP_026050359.1 2893259..2894296(-) (pilT) [Vibrio vulnificus strain FDAARGOS_663]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGVPAFNHADVHRLVFEIMNDSQRSEFEEKLEVDFSFELPNVGR
FRVNAFNQSRGCSAVFRTIPTNIPTLEELECPQIFEKIAKCEKGLVLVTGPTGSGKSTTLAAMVDYINRNYNKHILTIED
PIEFVHQNQKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRIGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGKVAKEEVDAKIEIETIQF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=387395 FOB71_RS21375 WP_026050359.1 2893259..2894296(-) (pilT) [Vibrio vulnificus strain FDAARGOS_663]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAACGCATCAGATCTACATCTTTCTGCGGGTGTGCCTCCTAT
GGTACGCATAGATGGCGATGTAAGAAAGCTCGGTGTGCCCGCATTTAATCATGCTGATGTGCATCGTTTGGTTTTTGAGA
TCATGAACGATTCACAGCGCAGTGAGTTTGAAGAGAAATTGGAAGTCGATTTTTCCTTTGAATTGCCTAACGTAGGTCGC
TTTCGTGTTAACGCCTTCAACCAATCTCGTGGCTGCTCTGCGGTGTTTCGTACCATTCCAACCAATATCCCTACCTTGGA
AGAGCTTGAGTGCCCGCAAATTTTTGAAAAAATTGCGAAGTGTGAAAAAGGCTTGGTGCTGGTCACCGGGCCGACAGGTT
CAGGTAAATCAACCACGTTAGCGGCGATGGTGGATTACATTAACCGAAACTACAACAAACACATTCTGACCATTGAAGAT
CCGATTGAATTTGTTCACCAAAATCAGAAATGTTTGATTAACCAACGCGAAGTTCATCGCGACACTCACAGTTTTAAAAA
TGCGTTGCGCAGTGCCTTGCGTGAAGACCCGGATGTGATTTTAGTGGGTGAGTTACGTGACCAAGAGACCATCAGCCTAG
CGCTCACCGCGGCAGAAACGGGCCACTTGGTGTTTGGTACGTTGCACACCAGTAGTGCAGCAAAAACCATTGACCGTATT
ATTGATGTGTTCCCTGGCAGTGACAAAGACATGGTGCGCTCAATGCTTTCTGAATCACTGCGCGCCGTTATCGCCCAGAA
ACTGCTGAAACGCATTGGAGGCGGGCGAGTGGCGTGTCATGAAATCATGATGGCGACGCCTGCCATTCGTAACTTGATCC
GTGAAGATAAAGTGGCACAGATGTACTCCATTATCCAAACAGGGGCTGCGCACGGCATGCAGACGATGGAACAGAATGCG
CGTCAGTTGATGGCACAGGGCAAAGTGGCCAAAGAAGAAGTCGACGCCAAAATTGAGATTGAAACTATCCAGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.116

100

0.881
  pilT Vibrio cholerae strain A1552

88.116

100

0.881
  pilT Acinetobacter baumannii D1279779

72.892

96.232

0.701
  pilT Acinetobacter baumannii strain A118

72.892

96.232

0.701
  pilT Acinetobacter nosocomialis M2

72.59

96.232

0.699
  pilT Acinetobacter baylyi ADP1

72.477

94.783

0.687
  pilT Pseudomonas aeruginosa PAK

68.529

98.551

0.675
  pilT Pseudomonas stutzeri DSM 10701

69.139

97.681

0.675
  pilT Legionella pneumophila strain Lp02

68.58

95.942

0.658
  pilT Legionella pneumophila strain ERS1305867

68.58

95.942

0.658
  pilT Neisseria meningitidis 8013

66.066

96.522

0.638
  pilT Neisseria gonorrhoeae MS11

65.766

96.522

0.635
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.088

92.174

0.499
  pilU Pseudomonas stutzeri DSM 10701

42.515

96.812

0.412
  pilU Vibrio cholerae strain A1552

41.916

96.812

0.406
  pilU Acinetobacter baylyi ADP1

40

92.754

0.371

Multiple sequence alignment