Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FOB71_RS21370 Genome accession   NZ_CP044069
Coordinates   2892134..2893240 (-) Length   368 a.a.
NCBI ID   WP_026050358.1    Uniprot ID   -
Organism   Vibrio vulnificus strain FDAARGOS_663     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2887134..2898240
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOB71_RS21345 (FOB71_21340) - 2887709..2889043 (-) 1335 WP_017428734.1 hypothetical protein -
  FOB71_RS21350 (FOB71_21345) rsmE 2889280..2890011 (+) 732 WP_017428735.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  FOB71_RS21355 (FOB71_21350) gshB 2890026..2890979 (+) 954 WP_026050357.1 glutathione synthase -
  FOB71_RS21360 (FOB71_21355) - 2891034..2891597 (+) 564 WP_011079465.1 YqgE/AlgH family protein -
  FOB71_RS21365 (FOB71_21360) ruvX 2891619..2892041 (+) 423 WP_011079464.1 Holliday junction resolvase RuvX -
  FOB71_RS21370 (FOB71_21365) pilU 2892134..2893240 (-) 1107 WP_026050358.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FOB71_RS21375 (FOB71_21370) pilT 2893259..2894296 (-) 1038 WP_026050359.1 type IV pilus twitching motility protein PilT Machinery gene
  FOB71_RS21380 (FOB71_21375) - 2894321..2895031 (+) 711 WP_017428739.1 YggS family pyridoxal phosphate-dependent enzyme -
  FOB71_RS21385 (FOB71_21380) proC 2895066..2895884 (+) 819 WP_017428740.1 pyrroline-5-carboxylate reductase -
  FOB71_RS21390 (FOB71_21385) - 2895926..2896483 (+) 558 WP_013571095.1 YggT family protein -
  FOB71_RS21395 (FOB71_21390) yggU 2896483..2896773 (+) 291 WP_017428741.1 DUF167 family protein YggU -
  FOB71_RS21400 (FOB71_21395) - 2896816..2897247 (+) 432 WP_017428742.1 DUF4426 domain-containing protein -
  FOB71_RS21405 (FOB71_21400) - 2897258..2897860 (+) 603 WP_026050360.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41201.30 Da        Isoelectric Point: 6.7294

>NTDB_id=387394 FOB71_RS21370 WP_026050358.1 2892134..2893240(-) (pilU) [Vibrio vulnificus strain FDAARGOS_663]
MDLNKILEGMITLKASDLYITVGAPVLFRVDGELRPQGEKLSQIEVAQLLDGAMDEDRRADFRKSRESNFAIVRDSGRFR
VSAFYQRELPGAVIRRIETNIPTFEELKLPHVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNTHRGGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLKGVVGQQLIRDKNGKGRHGVFEVLLNSPRVSDLIRRGDLHELKMTMAKSKEVGMQTFDQSLY
TLVVEGKISEEDAMHSADSANDLRLMLKTQRGDISSPGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=387394 FOB71_RS21370 WP_026050358.1 2892134..2893240(-) (pilU) [Vibrio vulnificus strain FDAARGOS_663]
ATGGATTTAAACAAGATTTTAGAAGGGATGATCACGCTTAAAGCGTCGGATCTTTATATCACGGTTGGTGCCCCCGTGCT
GTTTCGTGTCGATGGTGAGCTGCGGCCGCAAGGCGAAAAATTATCGCAAATTGAAGTAGCTCAACTGCTGGATGGTGCGA
TGGACGAAGATCGGCGCGCTGACTTTCGCAAAAGCCGCGAGTCAAACTTTGCCATTGTGCGAGATTCGGGCCGTTTTCGT
GTCAGTGCCTTCTACCAACGAGAGCTTCCTGGTGCGGTGATTCGCCGCATTGAAACCAATATTCCAACCTTTGAGGAGCT
TAAGCTGCCTCATGTTTTGCAAGACTTAGCGATTGCCAAGCGTGGATTGGTGCTGGTGGTGGGGGCAACGGGCTCGGGTA
AATCGACCACCATGGCTGCGATGACAGGCTATCGAAATACCCACCGTGGTGGACACATTTTGACGGTGGAAGACCCAATT
GAATTTGTTCACGAACATAAACGCTGTATTGTCACCCAGCGAGAAGTCGGCTTGGATACGGAAAGCTATGAAGTCGCACT
AAAGAACTCGCTGCGTCAAGCGCCAGATATGATTCTGATTGGTGAGATTCGCAGTCGCGAAACCATGGAATACGCCATGA
CCTTTGCTGAAACGGGTCACTTGTGTATGGCGACACTGCATGCCAATAACGCCAACCAAGCACTGGAGCGCATCTTACAC
TTGGTGCCTAAAGAACAAAAAGAGCAGTTTTTGTTTGACCTTTCCATGAACTTAAAAGGGGTGGTGGGTCAGCAGTTGAT
TCGTGATAAAAATGGTAAAGGTCGTCACGGTGTGTTCGAGGTGTTACTTAACAGCCCGCGCGTGTCGGATTTGATCCGTC
GTGGCGATTTGCATGAGCTGAAAATGACTATGGCGAAGTCGAAAGAAGTCGGTATGCAGACCTTTGACCAATCACTCTAT
ACATTAGTAGTGGAAGGCAAAATCAGCGAAGAAGACGCCATGCACAGTGCCGATTCGGCCAACGATTTACGTTTGATGCT
GAAGACGCAGCGAGGTGATATTAGTTCCCCCGGCTCATTGGCGAACGTGAAGATTGATATGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

84.239

100

0.842
  pilU Pseudomonas stutzeri DSM 10701

59.143

95.109

0.563
  pilU Acinetobacter baylyi ADP1

54.416

95.38

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.753

88.043

0.394
  pilT Acinetobacter baylyi ADP1

42.229

92.663

0.391
  pilT Legionella pneumophila strain ERS1305867

41.888

92.12

0.386
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Legionella pneumophila strain Lp02

41.888

92.12

0.386
  pilT Acinetobacter baumannii D1279779

41.593

92.12

0.383
  pilT Acinetobacter baumannii strain A118

41.593

92.12

0.383
  pilT Acinetobacter nosocomialis M2

41.593

92.12

0.383
  pilT Pseudomonas stutzeri DSM 10701

40.413

92.12

0.372
  pilT Neisseria meningitidis 8013

40.06

90.217

0.361
  pilT Neisseria gonorrhoeae MS11

40.06

90.217

0.361
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.761

0.361
  pilT Vibrio cholerae strain A1552

39.82

90.761

0.361

Multiple sequence alignment