Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   F2A31_RS11285 Genome accession   NZ_CP043909
Coordinates   2447977..2449014 (+) Length   345 a.a.
NCBI ID   WP_004638216.1    Uniprot ID   N9GFN0
Organism   Acinetobacter suaedae strain C16S1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2442977..2454014
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F2A31_RS11275 (F2A31_11275) - 2446577..2447167 (+) 591 WP_150026457.1 hypothetical protein -
  F2A31_RS11280 (F2A31_11280) - 2447164..2447850 (-) 687 WP_150026458.1 YggS family pyridoxal phosphate-dependent enzyme -
  F2A31_RS11285 (F2A31_11285) pilT 2447977..2449014 (+) 1038 WP_004638216.1 type IV pilus twitching motility protein PilT Machinery gene
  F2A31_RS11290 (F2A31_11290) pilU 2449043..2450161 (+) 1119 WP_150026459.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  F2A31_RS11295 (F2A31_11295) fur 2450247..2450684 (-) 438 WP_075316014.1 ferric iron uptake transcriptional regulator -
  F2A31_RS11300 (F2A31_11300) - 2450796..2451194 (+) 399 WP_150026460.1 outer membrane protein assembly factor BamE -
  F2A31_RS11305 (F2A31_11305) - 2451251..2451571 (-) 321 WP_150026461.1 RnfH family protein -
  F2A31_RS11310 (F2A31_11310) - 2451571..2452653 (-) 1083 WP_150026462.1 hypothetical protein -
  F2A31_RS11315 (F2A31_11315) - 2452903..2453358 (-) 456 WP_150027793.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38356.96 Da        Isoelectric Point: 6.3479

>NTDB_id=386479 F2A31_RS11285 WP_004638216.1 2447977..2449014(+) (pilT) [Acinetobacter suaedae strain C16S1]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDFEEDLETDFSFEVPNIAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGTIFKEICDYPRGIVLVTGPTGSGKSTTLAAMIDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLVRENKVAQMYSAIQTGANYGMTTLDQSL
KSLVSKGLISPQTARTVAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=386479 F2A31_RS11285 WP_004638216.1 2447977..2449014(+) (pilT) [Acinetobacter suaedae strain C16S1]
ATGGATATTACAGAATTACTCGCCTTTTCAGTGAAAAACGGTGCTTCAGATTTACACCTTTCAGCAGGTATGCCACCGAT
GATTCGTGTCGATGGTGAAGTTCGTCGCATTAACTTACCAGCATTAGAACATAAAGATGTACACCGTTTAGTGTACGACA
TTATGAATGACAAGCAACGTCGTGATTTCGAAGAAGACCTTGAAACAGACTTTTCATTTGAAGTTCCAAACATCGCCCGT
TTCCGTGTGAATGCATTCAACCAAAACCGTGGTGCTGGTGCAGTATTCCGTACCATTCCATCGAAAGTTTTAACCATGGA
AGACTTGGGTTTAGGCACAATTTTCAAAGAAATTTGTGACTATCCACGTGGCATCGTACTGGTCACAGGGCCTACTGGTT
CTGGTAAATCAACCACCTTGGCTGCGATGATCGACTATATTAATGAAAATCGTTATGACCATATCTTAACAGTCGAAGAT
CCAATCGAATTTGTACATCAATCAAAAAAATGCCTAATTAACCAACGTGAAGTGCATCGTGATACGCATGGTTTTAACGA
GGCACTACGTTCAGCACTGCGTGAAGATCCAGATATTATTCTAGTTGGTGAGATGCGTGACTTAGAGACCATTCGCCTTG
CGCTCACCGCAGCAGAAACAGGTCACCTCGTATTTGGTACACTGCATACCACCTCAGCGGCAAAAACCATTGACCGTGTC
ATCGATGTATTCCCTGCTGAAGAAAAAGACATGGTTCGTGCTATGCTCTCTGAGTCATTACAAGCAGTAATTTCTCAAAC
CTTATTGAAAAAAAATGGCGGCGGTCGTGTTGCAGCGCATGAAATCATGATCGGAATTCCAGCGATTCGTAACCTCGTCC
GTGAAAACAAAGTCGCGCAAATGTATTCTGCAATTCAAACAGGTGCTAACTATGGTATGACGACCTTAGATCAAAGTCTA
AAAAGTCTTGTATCTAAAGGTCTTATTAGTCCACAAACAGCACGTACTGTCGCAAAACAACCAGAATCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9GFN0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

96.522

100

0.965
  pilT Acinetobacter baumannii D1279779

96.232

100

0.962
  pilT Acinetobacter baumannii strain A118

96.232

100

0.962
  pilT Acinetobacter baylyi ADP1

87.5

99.71

0.872
  pilT Pseudomonas aeruginosa PAK

83.721

99.71

0.835
  pilT Pseudomonas stutzeri DSM 10701

83.43

99.71

0.832
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.345

97.391

0.675
  pilT Vibrio cholerae strain A1552

69.345

97.391

0.675
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.513
  pilU Vibrio cholerae strain A1552

41.791

97.101

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.101

0.4
  pilU Acinetobacter baylyi ADP1

39.306

100

0.394

Multiple sequence alignment