Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   F2A31_RS11290 Genome accession   NZ_CP043909
Coordinates   2449043..2450161 (+) Length   372 a.a.
NCBI ID   WP_150026459.1    Uniprot ID   A0A5P1UXB2
Organism   Acinetobacter suaedae strain C16S1     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2444043..2455161
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F2A31_RS11275 (F2A31_11275) - 2446577..2447167 (+) 591 WP_150026457.1 hypothetical protein -
  F2A31_RS11280 (F2A31_11280) - 2447164..2447850 (-) 687 WP_150026458.1 YggS family pyridoxal phosphate-dependent enzyme -
  F2A31_RS11285 (F2A31_11285) pilT 2447977..2449014 (+) 1038 WP_004638216.1 type IV pilus twitching motility protein PilT Machinery gene
  F2A31_RS11290 (F2A31_11290) pilU 2449043..2450161 (+) 1119 WP_150026459.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  F2A31_RS11295 (F2A31_11295) fur 2450247..2450684 (-) 438 WP_075316014.1 ferric iron uptake transcriptional regulator -
  F2A31_RS11300 (F2A31_11300) - 2450796..2451194 (+) 399 WP_150026460.1 outer membrane protein assembly factor BamE -
  F2A31_RS11305 (F2A31_11305) - 2451251..2451571 (-) 321 WP_150026461.1 RnfH family protein -
  F2A31_RS11310 (F2A31_11310) - 2451571..2452653 (-) 1083 WP_150026462.1 hypothetical protein -
  F2A31_RS11315 (F2A31_11315) - 2452903..2453358 (-) 456 WP_150027793.1 bacteriohemerythrin -
  F2A31_RS11320 (F2A31_11320) - 2453630..2454478 (-) 849 WP_150026463.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41715.77 Da        Isoelectric Point: 5.9701

>NTDB_id=386480 F2A31_RS11290 WP_150026459.1 2449043..2450161(+) (pilU) [Acinetobacter suaedae strain C16S1]
MDFNDLLNLMIQQNASDLFITADVEPSMKINGQIVPVSKTKLSADIVGQLVNSIMSDKQREEFAETHECNFAITNREKTA
RFRVSAFQQRDMPGMILRKIETHIPSMDDLKLPEVLKELAMTKRGIIIFVGATGTGKSTSLASMIGYRNQNSKGHIITIE
DPIEFVHQHAGCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVLATLHANNANQALDR
IIHFFEADRHSQLYMDLSLNLKALVAQQLIPTPDGNARRAAIEILINTPLLADYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLSEEGADQLLNASRNITFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=386480 F2A31_RS11290 WP_150026459.1 2449043..2450161(+) (pilU) [Acinetobacter suaedae strain C16S1]
ATGGATTTTAATGATTTACTCAATTTGATGATTCAACAAAATGCATCTGATTTATTCATCACTGCTGATGTTGAACCATC
AATGAAAATTAACGGGCAAATTGTGCCTGTCTCTAAAACCAAGTTATCTGCGGATATTGTGGGGCAACTTGTCAATTCAA
TTATGAGTGACAAACAACGTGAGGAGTTTGCGGAGACACACGAATGTAACTTTGCGATTACCAATCGTGAAAAAACAGCA
CGCTTTCGTGTCAGTGCATTTCAGCAACGCGATATGCCTGGAATGATTTTACGTAAAATTGAAACCCATATTCCAAGCAT
GGATGATTTAAAGTTACCAGAAGTACTCAAAGAACTTGCGATGACTAAACGAGGTATTATTATCTTCGTTGGTGCAACAG
GTACGGGTAAATCAACCTCATTAGCTTCAATGATTGGTTATCGCAATCAAAATTCTAAAGGGCATATTATTACCATCGAA
GATCCAATTGAATTTGTCCATCAACATGCTGGCTGTATTGTGACGCAACGTGAAGTCGGGATTGATACAGAATCTTTTGA
AGTTGCATTAAAAAATACACTTCGTCAGGCGCCAGATGTCATTTTGATTGGTGAGATTCGTTCACGCGAAACCATGGACT
ATGCGATCGCCTTTGCTGAAACGGGTCATTTAGTCCTCGCCACCCTGCATGCGAACAACGCCAACCAAGCCCTCGATCGT
ATTATTCACTTCTTTGAAGCAGATCGTCATAGCCAGTTGTATATGGATTTATCCCTTAACTTAAAGGCATTGGTTGCTCA
ACAATTGATTCCAACACCTGACGGAAATGCACGTCGAGCTGCGATTGAAATTTTAATTAATACCCCATTATTAGCCGACT
ATATCCGTAAGGGTGAAATTCATGAGATCAAAGATCTAATGAAACGCTCTCGTGAATTAGGTATGCAAACCTTTGACCAA
GCTTTGTTTGATCTTTATAAAGCAGGACAAATCACTTACAAAGATGCGCTTAAACATGCAGACTCACCAAATGACTTACG
TCTCACCATCAAGTTATCCGAAGAAGGTGCCGATCAATTATTGAATGCAAGCCGCAACATTACCTTTGATGGTCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5P1UXB2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.72

100

0.78
  pilU Pseudomonas stutzeri DSM 10701

70.35

99.731

0.702
  pilU Vibrio cholerae strain A1552

54.054

99.462

0.538
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.385

97.849

0.395
  pilT Legionella pneumophila strain Lp02

41.42

90.86

0.376
  pilT Legionella pneumophila strain ERS1305867

41.42

90.86

0.376
  pilT Neisseria gonorrhoeae MS11

39.706

91.398

0.363
  pilT Neisseria meningitidis 8013

39.706

91.398

0.363
  pilT Pseudomonas aeruginosa PAK

39.017

93.011

0.363
  pilT Acinetobacter baumannii D1279779

39.067

92.204

0.36
  pilT Acinetobacter nosocomialis M2

39.067

92.204

0.36
  pilT Acinetobacter baumannii strain A118

39.067

92.204

0.36
  pilT Pseudomonas stutzeri DSM 10701

38.728

93.011

0.36

Multiple sequence alignment