Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   F1719_RS13665 Genome accession   NZ_CP043852
Coordinates   2727098..2728078 (-) Length   326 a.a.
NCBI ID   WP_073984187.1    Uniprot ID   -
Organism   Escherichia coli ATCC 8739     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2722098..2733078
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F1719_RS13635 (F1719_13960) yggI 2722762..2723259 (+) 498 WP_001300769.1 SprT family zinc-dependent metalloprotease -
  F1719_RS13640 (F1719_13965) endA 2723354..2724061 (+) 708 WP_063116340.1 deoxyribonuclease I -
  F1719_RS13645 (F1719_13970) rsmE 2724141..2724872 (+) 732 WP_001300912.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  F1719_RS13650 (F1719_13975) gshB 2724885..2725835 (+) 951 WP_000593273.1 glutathione synthase -
  F1719_RS13655 (F1719_13980) yqgE 2725944..2726507 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  F1719_RS13660 (F1719_13985) ruvX 2726507..2726923 (+) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  F1719_RS13665 (F1719_13990) pilT 2727098..2728078 (-) 981 WP_073984187.1 type IV pilus twitching motility protein PilT Machinery gene
  F1719_RS13670 (F1719_13995) yggS 2728096..2728800 (+) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  F1719_RS13675 (F1719_14000) yggT 2728818..2729384 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  F1719_RS13680 (F1719_14005) yggU 2729381..2729671 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  F1719_RS13685 (F1719_14010) rdgB 2729679..2730272 (+) 594 WP_063116339.1 XTP/dITP diphosphatase -
  F1719_RS13690 (F1719_14015) hemW 2730265..2731401 (+) 1137 WP_000239922.1 radical SAM family heme chaperone HemW -
  F1719_RS13695 (F1719_14020) yggM 2731556..2732563 (-) 1008 WP_000745244.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35969.15 Da        Isoelectric Point: 5.9549

>NTDB_id=386278 F1719_RS13665 WP_073984187.1 2727098..2728078(-) (pilT) [Escherichia coli ATCC 8739]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDTPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRHGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=386278 F1719_RS13665 WP_073984187.1 2727098..2728078(-) (pilT) [Escherichia coli ATCC 8739]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGATACGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC
AGCGCATTCGCGCAACGGCATGGCATTTCGCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTTTCAGCAGCGA
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(61-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

38.554

100

0.393
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362

Multiple sequence alignment