Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   F0Q04_RS00320 Genome accession   NZ_CP043575
Coordinates   73246..74289 (+) Length   347 a.a.
NCBI ID   WP_021027363.1    Uniprot ID   A0AAW4Y2E3
Organism   Comamonas koreensis strain T50-37     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 68246..79289
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F0Q04_RS00295 norR 68385..70004 (+) 1620 WP_182343912.1 nitric oxide reductase transcriptional regulator NorR -
  F0Q04_RS00300 - 70032..70634 (+) 603 WP_165841247.1 zinc-ribbon domain containing protein -
  F0Q04_RS00305 - 70693..71109 (-) 417 WP_116927672.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  F0Q04_RS00310 - 71192..72328 (-) 1137 WP_116927661.1 pyridoxal-phosphate-dependent aminotransferase family protein -
  F0Q04_RS00315 - 72434..73162 (-) 729 WP_182343914.1 YggS family pyridoxal phosphate-dependent enzyme -
  F0Q04_RS00320 pilT 73246..74289 (+) 1044 WP_021027363.1 type IV pilus twitching motility protein PilT Machinery gene
  F0Q04_RS00325 - 74397..75005 (+) 609 WP_021027362.1 cyclic nucleotide-binding domain-containing protein -
  F0Q04_RS00330 pilU 75079..76215 (+) 1137 WP_116927663.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  F0Q04_RS00335 - 76292..77218 (+) 927 WP_021027360.1 NAD(P)-dependent oxidoreductase -
  F0Q04_RS00340 - 77437..78096 (-) 660 Protein_63 BON domain-containing protein -
  F0Q04_RS00345 - 78096..78695 (-) 600 WP_116927665.1 SIS domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38356.03 Da        Isoelectric Point: 6.7744

>NTDB_id=385243 F0Q04_RS00320 WP_021027363.1 73246..74289(+) (pilT) [Comamonas koreensis strain T50-37]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDPLDHKQVHAMVYDIMSDAQRKQYEEFLEVDFSFEIEGLAR
FRVNAFNQNRGSGAVFRTIPSKILTLEQLNAPKIFADLSLKPRGLVLVTGPTGSGKSTTLAAMVNYLNENEYGHILTVED
PIEFVHESKKCLINQREVGPQTLSFSAALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTPAIRNLIREAKVAQMYSTIQTSNSMGMQTLDQN
LTDLVRRNVISPAEARAKAKTPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=385243 F0Q04_RS00320 WP_021027363.1 73246..74289(+) (pilT) [Comamonas koreensis strain T50-37]
GTGGATATCACCCAATTGCTGGCATTCAGTGTCAAAAATAAAGCGTCGGACTTGCACCTGTCTGCAGGCCTGCCGCCGAT
GATCCGGGTCCATGGCGATGTGCGCCGCATCAATGTCGATCCGCTGGACCACAAACAGGTCCATGCGATGGTCTACGACA
TCATGAGCGATGCCCAGCGCAAGCAGTATGAAGAGTTTCTCGAGGTCGACTTCTCGTTCGAGATCGAAGGCCTGGCCCGC
TTCCGTGTCAATGCTTTCAACCAGAACCGCGGCTCGGGTGCCGTGTTCCGGACCATTCCGAGCAAGATCCTGACCCTCGA
GCAGCTCAACGCCCCCAAGATCTTTGCCGACCTGTCGCTCAAGCCGCGCGGTCTGGTGCTGGTGACCGGGCCGACCGGCT
CGGGCAAGTCCACCACCTTGGCGGCCATGGTCAACTACCTCAACGAAAACGAATACGGCCACATCCTGACGGTGGAAGAC
CCAATCGAATTCGTGCACGAGTCCAAGAAATGCCTGATCAACCAGCGCGAAGTGGGCCCGCAGACGCTGTCGTTCAGCGC
CGCGCTGCGCTCTGCGCTGCGTGAAGACCCGGACGCGATCCTGGTGGGCGAAATGCGTGACCTCGAAACCATCCGCCTGG
CGATGACGGCCGCAGAAACCGGCCACTTGGTGTTCGGCACCCTGCACACCTCCAGCGCCGCCAAGACCATTGACCGGATC
ATCGACGTCTTCCCCGCCGAAGAAAAGGAAATGGTCCGCGCGATGCTGTCCGAATCGCTGCAGGCTGTGATCTCCCAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGCGTGGCCGCCCACGAGATCATGCTGGGCACGCCCGCCATCCGCAACCTGA
TCCGTGAGGCCAAGGTCGCGCAGATGTATTCCACCATCCAGACCAGCAATAGCATGGGCATGCAGACGCTGGACCAGAAC
CTGACCGACCTGGTGCGCCGCAATGTGATCAGCCCGGCCGAGGCCCGCGCCAAGGCGAAGACGCCGGAGAACTTCCCGGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.971

99.424

0.775
  pilT Acinetobacter nosocomialis M2

77.391

99.424

0.769
  pilT Acinetobacter baumannii D1279779

77.391

99.424

0.769
  pilT Acinetobacter baumannii strain A118

77.391

99.424

0.769
  pilT Pseudomonas stutzeri DSM 10701

76.812

99.424

0.764
  pilT Acinetobacter baylyi ADP1

75.652

99.424

0.752
  pilT Legionella pneumophila strain Lp02

73.156

97.695

0.715
  pilT Legionella pneumophila strain ERS1305867

73.156

97.695

0.715
  pilT Neisseria meningitidis 8013

67.536

99.424

0.671
  pilT Neisseria gonorrhoeae MS11

67.246

99.424

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.343

97.406

0.666
  pilT Vibrio cholerae strain A1552

68.343

97.406

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.424

0.507
  pilU Vibrio cholerae strain A1552

42.899

99.424

0.427
  pilU Acinetobacter baylyi ADP1

41.618

99.712

0.415
  pilU Pseudomonas stutzeri DSM 10701

41.159

99.424

0.409

Multiple sequence alignment