Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   F0Q04_RS00330 Genome accession   NZ_CP043575
Coordinates   75079..76215 (+) Length   378 a.a.
NCBI ID   WP_116927663.1    Uniprot ID   A0A363RD62
Organism   Comamonas koreensis strain T50-37     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 70079..81215
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F0Q04_RS00305 - 70693..71109 (-) 417 WP_116927672.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  F0Q04_RS00310 - 71192..72328 (-) 1137 WP_116927661.1 pyridoxal-phosphate-dependent aminotransferase family protein -
  F0Q04_RS00315 - 72434..73162 (-) 729 WP_182343914.1 YggS family pyridoxal phosphate-dependent enzyme -
  F0Q04_RS00320 pilT 73246..74289 (+) 1044 WP_021027363.1 type IV pilus twitching motility protein PilT Machinery gene
  F0Q04_RS00325 - 74397..75005 (+) 609 WP_021027362.1 cyclic nucleotide-binding domain-containing protein -
  F0Q04_RS00330 pilU 75079..76215 (+) 1137 WP_116927663.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  F0Q04_RS00335 - 76292..77218 (+) 927 WP_021027360.1 NAD(P)-dependent oxidoreductase -
  F0Q04_RS00340 - 77437..78096 (-) 660 Protein_63 BON domain-containing protein -
  F0Q04_RS00345 - 78096..78695 (-) 600 WP_116927665.1 SIS domain-containing protein -
  F0Q04_RS00350 - 78884..79330 (-) 447 WP_182343916.1 YraN family protein -
  F0Q04_RS00355 rsmI 79321..80253 (+) 933 WP_182343919.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41985.36 Da        Isoelectric Point: 6.7686

>NTDB_id=385244 F0Q04_RS00330 WP_116927663.1 75079..76215(+) (pilU) [Comamonas koreensis strain T50-37]
MERDQASKFINDLLKLMVQRNGSDLFITADFPPAIKIDGKVTKVSSQPLSPTHSLTLARSIMSDKQVAEFEKTKECNFAI
SPSGIGRFRVNAFMQQGHVGLVLRTIPLTLPTIDGLGVPQVLKDLAMTKRGLCIMVGATGSGKSTTLAAMVDWRNENSYG
HIITVEDPVEFVHPHKNCIVTQREVGLDTDNWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRGLVSQRLLSKQDSKGRVAAVEVMLNSPLISDMIFKGEVGEIKEIMKKSRNLG
MQTFDQALFDLYEANMITYEDALRNADSVNDLRLQIKLGSQRAKRSDLAEGTEHLTIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=385244 F0Q04_RS00330 WP_116927663.1 75079..76215(+) (pilU) [Comamonas koreensis strain T50-37]
ATGGAACGGGATCAGGCCAGTAAGTTCATCAACGACCTGTTGAAGCTGATGGTGCAGCGCAATGGCAGCGATTTGTTCAT
CACCGCTGACTTTCCGCCGGCCATCAAGATCGATGGCAAGGTCACCAAGGTCTCCAGCCAGCCGCTGTCGCCCACCCACT
CGCTGACGCTGGCCCGCTCGATCATGAGCGACAAGCAGGTCGCCGAGTTCGAAAAGACCAAGGAATGCAATTTCGCGATC
TCGCCCTCGGGCATCGGGCGCTTCCGGGTCAATGCCTTCATGCAGCAAGGCCATGTGGGTCTGGTACTGCGGACCATTCC
ATTGACCCTGCCAACGATTGACGGCCTGGGCGTGCCCCAGGTGCTCAAGGACCTGGCGATGACCAAGCGCGGCCTGTGCA
TCATGGTGGGCGCCACCGGCTCGGGCAAGTCCACCACCTTGGCGGCGATGGTCGACTGGCGCAACGAAAACTCCTACGGC
CACATCATCACCGTCGAAGACCCGGTGGAATTTGTCCACCCGCACAAGAACTGCATCGTGACCCAGCGCGAAGTGGGCCT
GGACACCGACAACTGGGAAGCGGCGCTGAAAAACACCTTGCGCCAGGCGCCCGACGTCATCCTGATGGGCGAAATCCGCG
ACCGCGAAACCATGGAGCACGCGGTGGCCTTTGCCGAAACCGGTCACCTGTGCCTGGCCACCCTGCACGCCAACAGCGCC
AACCAGGCGCTCGACCGGATCATCAACTTCTTCCCCGAAGAGCGCCGCGCGCAGCTGCTGATGGACCTGTCGCTCAACCT
GCGCGGCCTGGTGTCGCAGCGCCTTTTGAGCAAGCAGGACAGCAAGGGCCGCGTCGCCGCCGTCGAGGTAATGCTCAACT
CGCCGCTGATCTCCGACATGATCTTCAAGGGCGAAGTGGGCGAGATCAAGGAGATCATGAAGAAAAGCCGCAACCTGGGC
ATGCAGACCTTTGACCAGGCGCTGTTTGACCTCTACGAGGCCAACATGATCACCTACGAAGACGCGCTGCGCAACGCCGA
CTCGGTCAACGACCTGCGCCTGCAGATCAAGCTGGGCAGCCAGCGCGCCAAGCGCAGTGACCTGGCCGAGGGCACGGAGC
ACCTGACAATCGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-251)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A363RD62

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.568

96.825

0.606
  pilU Acinetobacter baylyi ADP1

57.382

94.974

0.545
  pilU Vibrio cholerae strain A1552

53.911

94.709

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.51

89.153

0.397
  pilT Pseudomonas aeruginosa PAK

43.027

89.153

0.384
  pilT Acinetobacter baumannii D1279779

43.243

88.095

0.381
  pilT Acinetobacter nosocomialis M2

43.243

88.095

0.381
  pilT Acinetobacter baumannii strain A118

43.243

88.095

0.381
  pilT Legionella pneumophila strain ERS1305867

42.943

88.095

0.378
  pilT Legionella pneumophila strain Lp02

42.943

88.095

0.378
  pilT Acinetobacter baylyi ADP1

42.342

88.095

0.373
  pilT Pseudomonas stutzeri DSM 10701

41.543

89.153

0.37

Multiple sequence alignment