Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FYK34_RS17635 Genome accession   NZ_CP043473
Coordinates   3719706..3720743 (-) Length   345 a.a.
NCBI ID   WP_149298732.1    Uniprot ID   A0A5C1DKS6
Organism   Chromobacterium paludis strain IIBBL 257-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3714706..3725743
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FYK34_RS17620 (FYK34_17620) - 3715149..3716762 (-) 1614 WP_149298725.1 response regulator -
  FYK34_RS17625 (FYK34_17625) - 3716839..3718452 (-) 1614 WP_149298728.1 response regulator -
  FYK34_RS17630 (FYK34_17630) pilU 3718478..3719614 (-) 1137 WP_149298730.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FYK34_RS17635 (FYK34_17635) pilT 3719706..3720743 (-) 1038 WP_149298732.1 type IV pilus twitching motility protein PilT Machinery gene
  FYK34_RS17640 (FYK34_17640) - 3720993..3721688 (+) 696 WP_149298734.1 YggS family pyridoxal phosphate-dependent enzyme -
  FYK34_RS17645 (FYK34_17645) proC 3721742..3722545 (+) 804 WP_149298736.1 pyrroline-5-carboxylate reductase -
  FYK34_RS17650 (FYK34_17650) - 3722555..3723139 (+) 585 WP_149298738.1 YggT family protein -
  FYK34_RS17655 (FYK34_17655) - 3723217..3723522 (+) 306 WP_149298740.1 c-type cytochrome -
  FYK34_RS17660 (FYK34_17660) dksA 3723808..3724218 (+) 411 WP_043627703.1 RNA polymerase-binding protein DksA -
  FYK34_RS17665 (FYK34_17665) aroG 3724362..3725423 (+) 1062 WP_149298742.1 3-deoxy-7-phosphoheptulonate synthase AroG -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38217.91 Da        Isoelectric Point: 6.7262

>NTDB_id=384398 FYK34_RS17635 WP_149298732.1 3719706..3720743(-) (pilT) [Chromobacterium paludis strain IIBBL 257-1]
MEISELLAFTVKNKASDLHLSAGLPPMIRVNGDIRRINLPPMDHHDVHDMVYDIMNDYQRKIFEDSYECDFSFELPGIAR
FRVNAFVQNRGMAAVFRVIPSKVLTLEQLSAPKIFQDIANFPRGLVLVTGPTGSGKSTTLAAMIDYVNENSFSHILTVED
PIEFVHESKKSLINQRELGLQTHSFANALKSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAGEKEMVRSMLSESVRAVIAQTLLKTKDGAGRVAAHEIMMGTPAIRNLIRENKIAQINSMIQTGQQHGMQTLDQC
LSDLVRRNLVSPGEARAKASNKDAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=384398 FYK34_RS17635 WP_149298732.1 3719706..3720743(-) (pilT) [Chromobacterium paludis strain IIBBL 257-1]
ATGGAAATCTCCGAGCTGCTGGCATTCACCGTCAAGAACAAGGCCTCCGACCTGCACCTGTCGGCCGGCCTGCCGCCGAT
GATCCGCGTCAACGGCGACATCCGCCGCATCAATCTGCCGCCGATGGACCATCATGACGTGCACGACATGGTGTACGACA
TCATGAACGACTACCAGCGCAAGATCTTCGAGGACAGCTACGAGTGCGACTTCTCGTTCGAGCTGCCCGGCATCGCCCGT
TTCCGCGTCAACGCCTTCGTGCAAAACCGCGGCATGGCGGCGGTGTTTCGGGTGATTCCAAGCAAGGTGCTGACGCTGGA
GCAGCTGTCCGCGCCCAAGATTTTCCAGGACATCGCCAACTTCCCGCGCGGCCTGGTGCTGGTCACCGGCCCCACCGGCT
CCGGCAAGTCCACCACGCTGGCGGCGATGATAGACTATGTCAACGAGAACTCTTTTTCGCACATTCTTACCGTGGAGGAC
CCAATCGAGTTCGTCCACGAGAGCAAGAAGAGCCTGATCAACCAGCGCGAACTGGGCCTGCAGACGCACAGCTTCGCCAA
CGCGCTGAAAAGCGCGCTGCGCGAGGACCCGGACGTGATCCTGGTGGGCGAATTGCGCGATCTGGAAACGATACGGTTGG
CGCTGACCGCGGCCGAGACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGCAGCGCGGCCAAGACCATAGACCGTATC
GTGGACGTATTCCCGGCCGGCGAGAAGGAAATGGTGCGCTCCATGCTGTCCGAATCGGTGCGCGCGGTCATCGCGCAGAC
ATTGCTGAAAACCAAGGACGGCGCGGGCCGCGTGGCGGCGCACGAGATCATGATGGGCACCCCGGCCATCCGCAACCTGA
TACGCGAAAACAAGATCGCCCAGATCAATTCGATGATACAGACCGGCCAGCAGCACGGCATGCAGACGCTGGACCAATGT
CTGTCTGACCTGGTGCGGCGCAATCTGGTGTCGCCGGGCGAAGCGCGCGCCAAGGCCAGCAACAAGGACGCCTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C1DKS6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

72.754

100

0.728
  pilT Acinetobacter nosocomialis M2

72.754

100

0.728
  pilT Acinetobacter baumannii strain A118

72.754

100

0.728
  pilT Neisseria meningitidis 8013

72.174

100

0.722
  pilT Neisseria gonorrhoeae MS11

72.174

100

0.722
  pilT Pseudomonas stutzeri DSM 10701

71.884

100

0.719
  pilT Pseudomonas aeruginosa PAK

71.594

100

0.716
  pilT Acinetobacter baylyi ADP1

71.014

100

0.71
  pilT Legionella pneumophila strain Lp02

70.145

100

0.701
  pilT Legionella pneumophila strain ERS1305867

70.145

100

0.701
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.751

97.971

0.664
  pilT Vibrio cholerae strain A1552

67.751

97.971

0.664
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.474

95.942

0.513
  pilU Pseudomonas stutzeri DSM 10701

43.519

93.913

0.409
  pilU Vibrio cholerae strain A1552

41.791

97.101

0.406
  pilU Acinetobacter baylyi ADP1

40.058

99.13

0.397

Multiple sequence alignment