Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FBAL_RS15095 Genome accession   NC_014541
Coordinates   3312497..3313531 (-) Length   344 a.a.
NCBI ID   WP_013346450.1    Uniprot ID   E1STB0
Organism   Ferrimonas balearica DSM 9799     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3307497..3318531
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FBAL_RS15070 (Fbal_2937) - 3308745..3309572 (-) 828 WP_013346445.1 mechanosensitive ion channel domain-containing protein -
  FBAL_RS15075 (Fbal_2938) - 3309572..3310072 (-) 501 WP_013346446.1 OmpA family protein -
  FBAL_RS15080 (Fbal_2939) - 3310116..3310712 (-) 597 WP_013346447.1 SOS response-associated peptidase family protein -
  FBAL_RS15085 (Fbal_2940) - 3310753..3311361 (-) 609 WP_013346448.1 DUF924 family protein -
  FBAL_RS15090 (Fbal_2941) pilU 3311372..3312487 (-) 1116 WP_013346449.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FBAL_RS15095 (Fbal_2942) pilT 3312497..3313531 (-) 1035 WP_013346450.1 type IV pilus twitching motility protein PilT Machinery gene
  FBAL_RS15100 (Fbal_2943) - 3313559..3314242 (+) 684 WP_013346451.1 YggS family pyridoxal phosphate-dependent enzyme -
  FBAL_RS15105 (Fbal_2944) proC 3314248..3315072 (+) 825 WP_013346452.1 pyrroline-5-carboxylate reductase -
  FBAL_RS15110 (Fbal_2945) - 3315091..3315639 (+) 549 WP_013346453.1 YggT family protein -
  FBAL_RS15115 (Fbal_2946) yggU 3315643..3315933 (+) 291 WP_013346454.1 DUF167 family protein YggU -
  FBAL_RS15120 (Fbal_2947) - 3315970..3316407 (+) 438 WP_013346455.1 DUF4426 domain-containing protein -
  FBAL_RS15125 (Fbal_2948) rdgB 3316512..3317108 (+) 597 WP_041251803.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  FBAL_RS15130 (Fbal_2949) hemW 3317109..3318248 (+) 1140 WP_013346457.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38096.52 Da        Isoelectric Point: 6.6217

>NTDB_id=38436 FBAL_RS15095 WP_013346450.1 3312497..3313531(-) (pilT) [Ferrimonas balearica DSM 9799]
MEITELLAFSVKHNASDLHLSAGVQPMIRVDGEVRRINLPQLDHKQVHALIFDIMNDRQRKEFEEHLEVDFSFEVPGLAR
FRVNAFNQNRGAGAVFRTIPSKVLTLDQLNAPDIFRDIASYPKGLVLVTGPTGSGKSTTLAAMVDYVNDNRHDHILTIED
PIEFVHDNKNCLINQREVHKDTHSFSNALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPAGEKDMVRAMLSESLQAVISQTLVKKVGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQSL
TNLVNRGEITRADALTKTANKNAL

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=38436 FBAL_RS15095 WP_013346450.1 3312497..3313531(-) (pilT) [Ferrimonas balearica DSM 9799]
ATGGAAATTACTGAACTACTGGCCTTTAGTGTAAAACACAACGCTTCGGATCTGCACCTCTCTGCCGGGGTACAACCGAT
GATTCGGGTGGATGGTGAGGTTCGACGGATCAACCTGCCCCAACTCGATCACAAACAGGTGCATGCGCTGATCTTTGACA
TTATGAACGACCGTCAGCGCAAGGAGTTTGAAGAGCACCTGGAGGTGGATTTCTCCTTCGAGGTGCCCGGCCTGGCTCGC
TTCCGTGTTAACGCCTTTAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGTACCATTCCCAGCAAAGTACTGACGCTGGA
CCAGCTCAACGCCCCGGACATCTTCCGCGATATCGCCTCCTATCCGAAAGGACTGGTATTGGTAACCGGCCCCACCGGCT
CCGGTAAAAGTACCACGCTGGCGGCGATGGTGGATTACGTCAATGACAACCGTCATGACCATATCCTCACCATTGAAGAC
CCCATCGAATTTGTCCACGACAACAAGAACTGCCTGATCAACCAGCGGGAAGTGCACAAGGACACCCACAGTTTCTCCAA
CGCCCTGCGCAGCGCCCTGCGTGAAGACCCGGATGTGATTCTGGTGGGTGAGATGCGTGACCTGGAAACCATCCGCCTGG
CCCTGACCGCGGCGGAAACCGGCCACCTGGTGTTTGGCACCCTGCACACCACCTCCGCCGCCAAAACCATCGACCGTATC
GTCGACGTGTTCCCTGCGGGTGAGAAGGACATGGTGCGGGCGATGTTGTCGGAATCCCTGCAGGCCGTTATCTCCCAGAC
TCTGGTCAAGAAAGTGGGCGGCGGCCGGGTCGCGGCGCACGAGATCATGATCGGCACCCCGGCCATCCGTAACCTGATCC
GCGAAGACAAGGTGGCGCAGATGTACTCCGCCATCCAGACCGGTATGGCCCATGGCATGCAGACCCTGGAGCAGAGTCTG
ACCAACCTGGTTAATCGCGGCGAAATCACCCGCGCAGACGCGCTGACCAAGACCGCCAACAAGAACGCGCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E1STB0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.952

97.674

0.791
  pilT Acinetobacter baumannii D1279779

80.952

97.674

0.791
  pilT Acinetobacter baumannii strain A118

80.952

97.674

0.791
  pilT Pseudomonas stutzeri DSM 10701

79.822

97.965

0.782
  pilT Pseudomonas aeruginosa PAK

79.525

97.965

0.779
  pilT Acinetobacter baylyi ADP1

80.851

95.64

0.773
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

74.481

97.965

0.73
  pilT Vibrio cholerae strain A1552

74.481

97.965

0.73
  pilT Legionella pneumophila strain Lp02

71.637

99.419

0.712
  pilT Legionella pneumophila strain ERS1305867

71.637

99.419

0.712
  pilT Neisseria meningitidis 8013

66.957

100

0.672
  pilT Neisseria gonorrhoeae MS11

66.667

100

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.183

98.256

0.503
  pilU Pseudomonas stutzeri DSM 10701

42.899

100

0.43
  pilU Vibrio cholerae strain A1552

41.159

100

0.413
  pilU Acinetobacter baylyi ADP1

41.04

100

0.413

Multiple sequence alignment