Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FBAL_RS15090 Genome accession   NC_014541
Coordinates   3311372..3312487 (-) Length   371 a.a.
NCBI ID   WP_013346449.1    Uniprot ID   E1STA9
Organism   Ferrimonas balearica DSM 9799     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3306372..3317487
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FBAL_RS15060 (Fbal_2934) yciA 3306937..3307329 (-) 393 WP_013346442.1 acyl-CoA thioester hydrolase YciA -
  FBAL_RS15065 (Fbal_2935) ltaE 3307329..3308339 (-) 1011 WP_013346443.1 low-specificity L-threonine aldolase -
  FBAL_RS15070 (Fbal_2937) - 3308745..3309572 (-) 828 WP_013346445.1 mechanosensitive ion channel domain-containing protein -
  FBAL_RS15075 (Fbal_2938) - 3309572..3310072 (-) 501 WP_013346446.1 OmpA family protein -
  FBAL_RS15080 (Fbal_2939) - 3310116..3310712 (-) 597 WP_013346447.1 SOS response-associated peptidase family protein -
  FBAL_RS15085 (Fbal_2940) - 3310753..3311361 (-) 609 WP_013346448.1 DUF924 family protein -
  FBAL_RS15090 (Fbal_2941) pilU 3311372..3312487 (-) 1116 WP_013346449.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FBAL_RS15095 (Fbal_2942) pilT 3312497..3313531 (-) 1035 WP_013346450.1 type IV pilus twitching motility protein PilT Machinery gene
  FBAL_RS15100 (Fbal_2943) - 3313559..3314242 (+) 684 WP_013346451.1 YggS family pyridoxal phosphate-dependent enzyme -
  FBAL_RS15105 (Fbal_2944) proC 3314248..3315072 (+) 825 WP_013346452.1 pyrroline-5-carboxylate reductase -
  FBAL_RS15110 (Fbal_2945) - 3315091..3315639 (+) 549 WP_013346453.1 YggT family protein -
  FBAL_RS15115 (Fbal_2946) yggU 3315643..3315933 (+) 291 WP_013346454.1 DUF167 family protein YggU -
  FBAL_RS15120 (Fbal_2947) - 3315970..3316407 (+) 438 WP_013346455.1 DUF4426 domain-containing protein -
  FBAL_RS15125 (Fbal_2948) rdgB 3316512..3317108 (+) 597 WP_041251803.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41514.48 Da        Isoelectric Point: 5.8979

>NTDB_id=38435 FBAL_RS15090 WP_013346449.1 3311372..3312487(-) (pilU) [Ferrimonas balearica DSM 9799]
MAIQSLLDSMLARKASDLFITVGFPPSAKIDGELQQLNSEPLDRDGALALVESVMNEDNRRQFHQQREANFAFSVPGQAA
RFRVSAFWQRDNPGMVMRSIETRIPSMEELHLPEIMKNVCMAKRGLVILVGATGTGKSTSLAAMVGYRNQHQKGHILTIE
DPIEFVHEHDQSIVTQREVGIDTESFDAALKSALRQAPDVILLGEIRSQETMEFALSFAETGHLCMATLHANNANQALDR
ILHLVPKEKHQQLLFDLSLNLRAIMAQQLVPHRDGKGRRAAIEVLLNTPRVSDLIARNQLFELKETMSKGREQGMQTFDQ
ALFDLYMADEISMVDALHYADSPNDLRLMIKLKQANHTGSGYMDGVTIDLD

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=38435 FBAL_RS15090 WP_013346449.1 3311372..3312487(-) (pilU) [Ferrimonas balearica DSM 9799]
ATGGCGATTCAATCCCTGCTTGATTCCATGCTGGCGCGCAAAGCGTCGGATCTGTTTATCACCGTAGGCTTTCCGCCCAG
CGCGAAAATTGACGGCGAACTGCAGCAGCTGAACAGTGAACCTCTCGACCGCGACGGGGCCCTGGCTCTGGTGGAAAGCG
TGATGAACGAGGACAACCGGCGCCAGTTCCACCAACAGCGTGAAGCCAACTTTGCGTTCAGCGTGCCGGGCCAGGCTGCC
CGCTTTCGTGTGTCGGCGTTCTGGCAGCGTGACAATCCGGGCATGGTGATGCGTTCCATCGAGACCCGCATCCCCTCCAT
GGAGGAGCTTCACCTGCCGGAGATCATGAAAAACGTCTGCATGGCCAAGCGGGGACTGGTGATTCTGGTGGGAGCCACCG
GCACCGGTAAATCCACCTCGCTGGCGGCGATGGTGGGGTATCGCAACCAGCATCAGAAGGGCCATATTCTCACCATCGAA
GACCCCATCGAATTTGTTCACGAACACGACCAGAGCATCGTGACTCAGCGTGAAGTGGGCATCGATACCGAATCCTTTGA
TGCGGCCCTGAAAAGCGCCCTGCGTCAGGCGCCGGACGTGATTCTGCTGGGGGAGATCCGCTCCCAGGAAACCATGGAGT
TTGCGCTGTCGTTTGCGGAAACCGGCCACCTCTGTATGGCCACCCTGCATGCCAACAACGCCAACCAGGCGCTGGACCGC
ATTCTCCACCTGGTGCCCAAGGAGAAACACCAGCAACTGCTGTTTGACCTCTCCCTCAACCTGCGCGCCATCATGGCGCA
GCAGTTGGTGCCGCACCGGGACGGCAAGGGGCGTCGCGCCGCCATCGAAGTGCTGCTGAACACCCCACGTGTGTCGGACC
TGATTGCCCGCAATCAGCTGTTTGAGCTGAAGGAAACCATGTCCAAGGGGCGTGAACAGGGGATGCAGACCTTTGATCAG
GCCCTGTTCGACCTCTATATGGCGGATGAGATCTCCATGGTGGATGCGCTGCATTACGCCGACAGCCCCAACGATCTGCG
CCTGATGATCAAGCTCAAGCAGGCCAACCACACCGGTTCCGGTTATATGGATGGCGTGACCATCGATCTGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E1STA9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

61.725

100

0.617
  pilU Pseudomonas stutzeri DSM 10701

59.943

94.879

0.569
  pilU Acinetobacter baylyi ADP1

59.322

95.418

0.566
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.808

90.027

0.412
  pilT Pseudomonas aeruginosa PAK

41.908

93.261

0.391
  pilT Pseudomonas stutzeri DSM 10701

41.04

93.261

0.383
  pilT Acinetobacter baumannii strain A118

41.108

92.453

0.38
  pilT Acinetobacter baylyi ADP1

41.108

92.453

0.38
  pilT Acinetobacter baumannii D1279779

41.108

92.453

0.38
  pilT Acinetobacter nosocomialis M2

41.108

92.453

0.38
  pilT Legionella pneumophila strain ERS1305867

42.169

89.488

0.377
  pilT Legionella pneumophila strain Lp02

42.169

89.488

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.438

86.253

0.375
  pilT Vibrio cholerae strain A1552

43.438

86.253

0.375
  pilT Neisseria gonorrhoeae MS11

42.154

87.601

0.369
  pilT Neisseria meningitidis 8013

42.154

87.601

0.369

Multiple sequence alignment