Detailed information NTG0038415

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: HELO_RS17095
• Genome accession: NC_014532
• Coordinates: 3661032..3662066 (-)
• Length: 344 a.a.
• NCBI ID: WP_013333867.1
• Uniprot ID: E1VAP8
• Organism: Halomonas elongata DSM 2581
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3656032..3667066

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HELO_RS17085 (HELO_4111)	tyrS	3658582..3659781 (-)	1200	WP_013333865.1	tyrosine--tRNA ligase	-
HELO_RS17090 (HELO_4112)	-	3659893..3661023 (+)	1131	WP_013333866.1	anhydro-N-acetylmuramic acid kinase	-
HELO_RS17095 (HELO_4113)	pilT	3661032..3662066 (-)	1035	WP_013333867.1	type IV pilus twitching motility protein PilT	Machinery gene
HELO_RS17100 (HELO_4114)	-	3662123..3662827 (+)	705	WP_013333868.1	YggS family pyridoxal phosphate-dependent enzyme	-
HELO_RS17105 (HELO_4115)	proC	3662888..3663712 (+)	825	WP_013333869.1	pyrroline-5-carboxylate reductase	-
HELO_RS17110 (HELO_4116)	-	3663739..3664332 (+)	594	WP_013333870.1	YggT family protein	-
HELO_RS17115 (HELO_4117)	-	3664375..3665532 (+)	1158	WP_013333871.1	homoserine O-acetyltransferase	-
HELO_RS17120 (HELO_4118)	metW	3665532..3666158 (+)	627	WP_041602709.1	methionine biosynthesis protein MetW	-
HELO_RS17125 (HELO_4119)	sfsA	3666165..3666896 (-)	732	WP_013333873.1	DNA/RNA nuclease SfsA	-

Sequence

Protein

• Length: 344 a.a.
• Molecular weight: 36829.38 Da
• Isoelectric Point: 6.6657

>NTDB_id=38415 HELO_RS17095 WP_013333867.1 3661032..3662066(-) (pilT) [Halomonas elongata DSM 2581]
MNITELLAASAKRGASDLHLSAGMAPMMRVDGELEALDGPPVDDPTLRRELRRLLGESHWATFESELEVDVALELPGVAR
FRGNVFQHGRGIGAVFRTIPGDVPGLAALGMADTLGALAELRRGLVLVTGPTGSGKSTTLAALVNAINETRREHILTIED
PVEFVHHGKRCLVSQREVSRDTRSFAAALRSALREDPDVIMVGEMRDLETMRLALTAAETGHLVLGTLHTLSAAKTIDRI
IDAFPGPEQGMVRAMLSESLQAVVSQVLLKRCGGGRVAAHEVMVATPAVRNLIREDKVAQLHSVLQTGAAQGMQTLDTAL
ARLVAEGVVAPDEAQSRARAPLRR

Nucleotide

• Length: 1035 bp

>NTDB_id=38415 HELO_RS17095 WP_013333867.1 3661032..3662066(-) (pilT) [Halomonas elongata DSM 2581]
ATGAATATTACCGAACTGCTGGCAGCCTCGGCAAAGCGGGGCGCCTCCGATCTGCATCTGTCGGCAGGCATGGCACCGAT
GATGCGCGTCGACGGCGAGCTGGAAGCGCTCGATGGGCCGCCCGTCGATGACCCGACCTTGCGCAGAGAACTTCGAAGAC
TGCTCGGTGAGTCGCATTGGGCAACCTTCGAGTCCGAGTTGGAAGTCGACGTTGCCCTGGAGCTTCCCGGCGTGGCGCGT
TTCCGGGGCAACGTCTTCCAGCATGGCCGGGGCATCGGCGCGGTGTTTCGCACCATCCCCGGCGATGTGCCTGGACTGGC
TGCGCTCGGCATGGCGGATACCCTGGGGGCTCTGGCCGAACTGCGACGCGGACTGGTGCTGGTGACGGGGCCCACTGGTT
CGGGCAAGAGCACGACCCTGGCGGCGCTGGTCAATGCCATCAACGAGACGCGCCGCGAGCACATCCTGACCATCGAGGAT
CCGGTGGAGTTCGTGCATCACGGCAAGCGGTGCCTGGTCAGCCAGCGCGAGGTGTCGCGGGATACCCGGAGCTTTGCGGC
GGCCTTGCGCAGCGCGCTGCGCGAGGATCCCGATGTCATCATGGTCGGTGAAATGCGCGACCTGGAGACCATGCGGCTGG
CGCTGACTGCCGCCGAGACGGGGCACCTGGTACTGGGAACCCTGCATACGCTCTCGGCGGCCAAGACCATCGATCGCATC
ATCGATGCCTTCCCCGGCCCCGAACAGGGCATGGTGCGGGCGATGCTGTCGGAATCCCTGCAGGCGGTCGTCTCGCAGGT
GCTGTTGAAGCGCTGTGGGGGCGGGCGCGTGGCGGCTCATGAAGTCATGGTCGCCACGCCGGCGGTGCGTAACCTGATTC
GCGAGGACAAGGTGGCGCAACTCCATTCGGTGCTGCAGACCGGCGCCGCCCAGGGCATGCAGACACTGGACACGGCGCTG
GCGCGGCTGGTGGCCGAGGGTGTGGTGGCGCCTGACGAGGCGCAGTCGCGGGCGCGCGCTCCCCTGAGGCGGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (13-268)

3D structure

• AlphaFold DB: E1VAP8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Acinetobacter baumannii D1279779	62.17	99.128	0.616
pilT	Acinetobacter baumannii strain A118	62.17	99.128	0.616
pilT	Pseudomonas stutzeri DSM 10701	62.17	99.128	0.616
pilT	Pseudomonas aeruginosa PAK	61.877	99.128	0.613
pilT	Acinetobacter nosocomialis M2	61.877	99.128	0.613
pilT	Acinetobacter baylyi ADP1	60.704	99.128	0.602
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	60.35	99.709	0.602
pilT	Vibrio cholerae strain A1552	60.35	99.709	0.602
pilT	Neisseria meningitidis 8013	59.118	98.837	0.584
pilT	Neisseria gonorrhoeae MS11	58.824	98.837	0.581
pilT	Legionella pneumophila strain Lp02	58.284	98.256	0.573
pilT	Legionella pneumophila strain ERS1305867	58.284	98.256	0.573
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	48.538	99.419	0.483
pilU	Pseudomonas stutzeri DSM 10701	39.701	97.384	0.387
pilU	Vibrio cholerae strain A1552	38.806	97.384	0.378
pilB	Acinetobacter baylyi ADP1	32.061	100	0.366
pilU	Acinetobacter baylyi ADP1	36.152	99.709	0.36