Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FZF10_RS18600 Genome accession   NZ_CP043440
Coordinates   3579582..3580625 (+) Length   347 a.a.
NCBI ID   WP_149137443.1    Uniprot ID   -
Organism   Cupriavidus campinensis strain MJ1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3574582..3585625
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FZF10_RS18570 - 3574983..3575939 (+) 957 WP_149137438.1 LysR substrate-binding domain-containing protein -
  FZF10_RS18575 - 3576142..3576336 (+) 195 WP_149137439.1 hypothetical protein -
  FZF10_RS18580 - 3576426..3576911 (+) 486 WP_092298875.1 Dps family protein -
  FZF10_RS18585 ubiA 3577000..3577893 (+) 894 WP_149137440.1 4-hydroxybenzoate octaprenyltransferase -
  FZF10_RS18590 proC 3577915..3578745 (-) 831 WP_149137441.1 pyrroline-5-carboxylate reductase -
  FZF10_RS18595 - 3578827..3579516 (-) 690 WP_149137442.1 YggS family pyridoxal phosphate-dependent enzyme -
  FZF10_RS18600 pilT 3579582..3580625 (+) 1044 WP_149137443.1 type IV pilus twitching motility protein PilT Machinery gene
  FZF10_RS18605 pilU 3580641..3581780 (+) 1140 WP_149137444.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FZF10_RS18610 - 3581842..3582336 (+) 495 WP_149137445.1 glutathione peroxidase -
  FZF10_RS18615 - 3582360..3583052 (-) 693 WP_144202478.1 YafY family protein -
  FZF10_RS18620 - 3583218..3583943 (-) 726 WP_149137446.1 LrgB family protein -
  FZF10_RS18625 - 3583940..3584338 (-) 399 WP_149137447.1 CidA/LrgA family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38378.17 Da        Isoelectric Point: 6.8705

>NTDB_id=384112 FZF10_RS18600 WP_149137443.1 3579582..3580625(+) (pilT) [Cupriavidus campinensis strain MJ1]
MDIAQLLAFAVKNKASDLHLSADMPPMVRIHGDMRRINVAAMGHKDVHAMVYDIMSDVQRKSYEERLEVDFSFEIAGLSR
FRVNAYTTQRGSAAVFRTIPSKVLTLDDLHAPAVFADLCMKPRGLVLVTGPTGSGKSTTLAAMVDHRNNEDMGHILTVED
PIEFVHNSKKSLINQRELGPHTHSFANALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFATLHTSSAAKTIDRV
VDVFPPEEKDMVRTMLSESLEAVISQTLLKTRDGNGRTAAHEIMIATPAIRHLIRENKIAQMYSMMQTSSGMGMQTLDQC
LSDLIKRGMINYNDARAIAKNPDAFMG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=384112 FZF10_RS18600 WP_149137443.1 3579582..3580625(+) (pilT) [Cupriavidus campinensis strain MJ1]
ATGGACATCGCGCAGCTATTGGCTTTTGCGGTCAAGAACAAGGCATCCGATCTCCACCTCTCGGCAGACATGCCGCCGAT
GGTGCGTATTCACGGGGATATGCGTCGGATCAACGTGGCGGCCATGGGCCACAAGGACGTCCACGCCATGGTGTACGACA
TCATGAGCGACGTTCAGCGCAAGAGCTACGAAGAACGCCTCGAGGTCGACTTCTCGTTCGAGATTGCCGGCCTGTCACGG
TTCCGGGTCAATGCCTACACCACGCAGCGCGGTTCTGCCGCCGTGTTCCGGACCATTCCGTCGAAAGTCCTGACGCTCGA
TGACCTCCATGCACCGGCCGTGTTCGCGGACCTCTGCATGAAGCCACGCGGCCTCGTGCTCGTGACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCCATGGTCGATCACCGCAACAACGAGGACATGGGTCACATCCTCACGGTGGAAGAC
CCGATCGAATTCGTCCACAACTCCAAGAAAAGCCTGATCAACCAGCGCGAACTGGGCCCGCACACGCATTCGTTCGCGAA
CGCGCTGCGCTCGGCACTGCGTGAGGATCCGGACGTGATCCTGGTGGGCGAACTGCGTGACCTGGAAACCATCCGGCTGG
CGCTGACCGCCGCCGAAACCGGCCACCTGGTCTTCGCCACGCTGCACACAAGCTCCGCCGCCAAGACTATCGACCGGGTC
GTGGACGTGTTCCCGCCCGAGGAAAAGGACATGGTGCGGACCATGCTGTCGGAATCGCTCGAGGCCGTGATCTCCCAGAC
CCTGCTCAAGACGCGCGACGGCAACGGCCGGACCGCGGCGCACGAGATCATGATCGCCACGCCCGCCATCCGCCACCTGA
TCCGCGAGAACAAGATCGCGCAGATGTATTCGATGATGCAGACCTCGAGCGGCATGGGCATGCAGACGCTCGACCAGTGC
CTGAGCGACCTGATAAAGCGCGGCATGATCAACTACAACGACGCGCGGGCGATTGCCAAGAATCCCGACGCCTTCATGGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

70.52

99.712

0.703
  pilT Acinetobacter baumannii D1279779

70.52

99.712

0.703
  pilT Acinetobacter baumannii strain A118

70.52

99.712

0.703
  pilT Acinetobacter baylyi ADP1

69.275

99.424

0.689
  pilT Pseudomonas stutzeri DSM 10701

69.275

99.424

0.689
  pilT Pseudomonas aeruginosa PAK

68.406

99.424

0.68
  pilT Neisseria gonorrhoeae MS11

66.57

99.135

0.66
  pilT Neisseria meningitidis 8013

66.57

99.135

0.66
  pilT Legionella pneumophila strain Lp02

65.797

99.424

0.654
  pilT Legionella pneumophila strain ERS1305867

65.797

99.424

0.654
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.579

97.118

0.637
  pilT Vibrio cholerae strain A1552

65.579

97.118

0.637
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.986

99.424

0.487
  pilU Acinetobacter baylyi ADP1

40.698

99.135

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.299

96.542

0.389
  pilU Vibrio cholerae strain A1552

40

96.542

0.386

Multiple sequence alignment