Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FZF10_RS18605 Genome accession   NZ_CP043440
Coordinates   3580641..3581780 (+) Length   379 a.a.
NCBI ID   WP_149137444.1    Uniprot ID   -
Organism   Cupriavidus campinensis strain MJ1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3575641..3586780
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FZF10_RS18575 - 3576142..3576336 (+) 195 WP_149137439.1 hypothetical protein -
  FZF10_RS18580 - 3576426..3576911 (+) 486 WP_092298875.1 Dps family protein -
  FZF10_RS18585 ubiA 3577000..3577893 (+) 894 WP_149137440.1 4-hydroxybenzoate octaprenyltransferase -
  FZF10_RS18590 proC 3577915..3578745 (-) 831 WP_149137441.1 pyrroline-5-carboxylate reductase -
  FZF10_RS18595 - 3578827..3579516 (-) 690 WP_149137442.1 YggS family pyridoxal phosphate-dependent enzyme -
  FZF10_RS18600 pilT 3579582..3580625 (+) 1044 WP_149137443.1 type IV pilus twitching motility protein PilT Machinery gene
  FZF10_RS18605 pilU 3580641..3581780 (+) 1140 WP_149137444.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FZF10_RS18610 - 3581842..3582336 (+) 495 WP_149137445.1 glutathione peroxidase -
  FZF10_RS18615 - 3582360..3583052 (-) 693 WP_144202478.1 YafY family protein -
  FZF10_RS18620 - 3583218..3583943 (-) 726 WP_149137446.1 LrgB family protein -
  FZF10_RS18625 - 3583940..3584338 (-) 399 WP_149137447.1 CidA/LrgA family protein -
  FZF10_RS18630 glcF 3584399..3585649 (-) 1251 WP_144202474.1 glycolate oxidase subunit GlcF -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42402.64 Da        Isoelectric Point: 6.3204

>NTDB_id=384113 FZF10_RS18605 WP_149137444.1 3580641..3581780(+) (pilU) [Cupriavidus campinensis strain MJ1]
MLDRESAAKYINDLLELMVNNRGSDLFITSEFPPAIKVDGKITPVSQQPLNPAQALGLVRSIMNERQVKEFDESRECNFA
ITAPNAGRFRVSAFIQQGKAGMVVRTINTRIPSVEDLDLPTSLHEIVMAKRGLVIVTGATGSGKSTSLAAMLDHRNAHSY
GHIITIEDPIEYVHAHQNCIVTQREVGIDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAMQYAETGHLCLATLHANN
ANQAIDRIINFFPEEKRQQLLIDLSLNLKAMISQRLLPRAGRKGRVPAVEIMVGTPLVADLIFKGEIHELKEVIKKSREQ
GMISFDQALFELYEEEKITYEDALKNADSLNDLRLMIKLHSARHRDADLGAGTEHLNVI

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=384113 FZF10_RS18605 WP_149137444.1 3580641..3581780(+) (pilU) [Cupriavidus campinensis strain MJ1]
ATGCTCGACCGCGAATCCGCCGCGAAGTACATCAACGACCTGCTGGAGCTGATGGTCAACAACCGCGGCTCGGACCTGTT
CATCACGTCCGAATTCCCGCCGGCCATCAAGGTGGACGGCAAGATCACGCCCGTCTCGCAGCAGCCGCTCAACCCGGCAC
AGGCGCTGGGCCTGGTCCGCTCGATCATGAACGAGCGCCAGGTGAAGGAATTCGACGAAAGCCGCGAGTGCAACTTCGCC
ATCACCGCGCCGAATGCCGGGCGCTTCCGCGTCTCGGCCTTCATCCAGCAGGGCAAGGCCGGCATGGTGGTGCGGACGAT
CAACACGCGCATCCCGTCGGTGGAAGACCTGGACCTGCCCACGTCGCTCCATGAGATCGTCATGGCCAAGCGCGGGCTGG
TGATCGTGACCGGCGCCACGGGCTCGGGCAAGTCCACCTCGCTGGCGGCCATGCTCGACCACCGCAACGCGCACTCGTAC
GGCCACATCATCACGATCGAGGACCCGATCGAGTACGTGCACGCGCACCAGAACTGCATTGTCACGCAGCGCGAGGTCGG
CATCGACACCGAGTCGTGGCACGTGGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAAATCC
GCGACCGCGACACCATGGAATACGCGATGCAGTACGCCGAGACGGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAAC
GCCAACCAGGCCATCGACCGGATCATCAACTTCTTCCCCGAGGAGAAGCGCCAGCAACTGCTGATCGACCTGTCGCTGAA
CCTCAAGGCGATGATTTCGCAGCGGCTGCTGCCGCGCGCCGGCCGCAAGGGCCGCGTGCCGGCCGTGGAGATCATGGTGG
GCACGCCGCTGGTGGCCGACCTGATCTTCAAGGGCGAGATCCACGAGCTCAAGGAAGTCATCAAGAAGTCCCGCGAGCAG
GGCATGATCTCCTTCGACCAGGCGCTGTTCGAGCTGTACGAGGAAGAGAAGATCACCTACGAGGACGCGCTCAAGAACGC
CGACTCGCTGAACGACCTGCGCCTGATGATCAAGCTCCACAGCGCCCGCCACCGCGACGCCGACCTGGGCGCCGGCACCG
AGCACCTGAACGTCATTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(37-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.506

91.821

0.583
  pilU Acinetobacter baylyi ADP1

58.571

92.348

0.541
  pilU Vibrio cholerae strain A1552

54.27

95.778

0.52
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.214

88.918

0.393
  pilT Legionella pneumophila strain Lp02

44.444

87.863

0.39
  pilT Legionella pneumophila strain ERS1305867

44.444

87.863

0.39
  pilT Pseudomonas stutzeri DSM 10701

42.73

88.918

0.38
  pilT Acinetobacter nosocomialis M2

42.943

87.863

0.377
  pilT Acinetobacter baylyi ADP1

44.099

84.96

0.375
  pilT Pseudomonas aeruginosa PAK

42.136

88.918

0.375
  pilT Acinetobacter baumannii D1279779

43.887

84.169

0.369
  pilT Acinetobacter baumannii strain A118

43.887

84.169

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.533

83.641

0.364
  pilT Vibrio cholerae strain A1552

43.533

83.641

0.364

Multiple sequence alignment