Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HF909_RS03950 Genome accession   NZ_CP051169
Coordinates   817410..818453 (+) Length   347 a.a.
NCBI ID   WP_193027655.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain RUN2474     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 812410..823453
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HF909_RS03915 (HF909_03970) - 813294..813497 (+) 204 WP_230642591.1 hypothetical protein -
  HF909_RS03920 (HF909_03975) - 813556..813798 (+) 243 WP_011002597.1 hypothetical protein -
  HF909_RS03925 (HF909_03980) - 813957..814457 (+) 501 WP_003271865.1 Dps family protein -
  HF909_RS03930 (HF909_03985) ubiA 814531..815406 (+) 876 WP_193027651.1 4-hydroxybenzoate octaprenyltransferase -
  HF909_RS03935 (HF909_03990) - 815403..815681 (-) 279 WP_193027652.1 hypothetical protein -
  HF909_RS03940 (HF909_03995) proC 815704..816528 (-) 825 WP_193027653.1 pyrroline-5-carboxylate reductase -
  HF909_RS03945 (HF909_04000) - 816563..817285 (-) 723 WP_193027654.1 YggS family pyridoxal phosphate-dependent enzyme -
  HF909_RS03950 (HF909_04005) pilT 817410..818453 (+) 1044 WP_193027655.1 type IV pilus twitching motility protein PilT Machinery gene
  HF909_RS03955 (HF909_04010) pilU 818506..819645 (+) 1140 WP_193027656.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HF909_RS03960 (HF909_04015) pilE 819840..820280 (+) 441 WP_193035852.1 type IV pilin protein Machinery gene
  HF909_RS03965 (HF909_04020) - 820284..820772 (+) 489 WP_016723684.1 Tfp pilus assembly protein FimT/FimU -
  HF909_RS03970 (HF909_04025) pilV 820769..821359 (+) 591 WP_193035854.1 type IV pilus modification protein PilV -
  HF909_RS03975 (HF909_04030) - 821356..822384 (+) 1029 WP_193035856.1 PilW family protein -
  HF909_RS03980 (HF909_04035) - 822388..822924 (+) 537 WP_193035858.1 PilX N-terminal domain-containing pilus assembly protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38257.82 Da        Isoelectric Point: 6.8705

>NTDB_id=382936 HF909_RS03950 WP_193027655.1 817410..818453(+) (pilT) [Ralstonia pseudosolanacearum strain RUN2474]
MDIAQLLAFSAKNKASDLHLSAGLPPMIRIHGDMRRINVPPLTHQDVHSMVYDIMSDVQRKHYEENLEADFSFEIPGLSR
FRVNAFNQNRGASAVFRTIPSKVLTLEDLKAPAVFSDLAMKPRGLVLVTGPTGSGKSTTLAAMVNHRNENDLGHILTVED
PIEFVHESKKSLINQRELGPHTHSFANALKSALREDPDVVLVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPSDEKDMVRTMLSESLEAVISQTLLKTRDGSGRVAAHEIMICTPAIRHLIRENKISQMYSMMQTSSGLGMQTLDQC
LSELIKRSAINYADARAIAKNPDAFAN

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=382936 HF909_RS03950 WP_193027655.1 817410..818453(+) (pilT) [Ralstonia pseudosolanacearum strain RUN2474]
ATGGACATCGCGCAGCTTCTGGCCTTCTCTGCCAAGAACAAAGCGTCTGATCTGCACTTGTCGGCGGGCTTGCCTCCGAT
GATCCGGATCCATGGCGACATGCGCCGTATCAACGTGCCGCCGCTCACGCACCAGGATGTCCACTCCATGGTGTACGACA
TCATGAGCGACGTGCAGCGCAAGCATTACGAAGAAAACCTGGAAGCGGACTTCTCGTTCGAGATTCCCGGTCTGTCGCGT
TTCCGGGTCAACGCCTTCAACCAGAACCGCGGCGCTTCCGCCGTGTTCCGGACGATTCCGTCCAAGGTGCTGACGCTGGA
AGACCTGAAGGCGCCGGCCGTCTTCTCCGACCTCGCCATGAAGCCGCGCGGCCTGGTGCTGGTGACGGGGCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTTAACCACCGCAACGAAAACGACCTGGGCCACATCCTCACGGTGGAAGAC
CCGATCGAATTCGTGCACGAATCCAAGAAGAGCCTGATCAACCAGCGCGAACTGGGGCCGCATACCCATTCGTTCGCCAA
CGCGCTGAAATCGGCGCTGCGGGAAGACCCGGACGTGGTCCTGGTCGGCGAATTGCGTGACCTGGAAACCATCCGCCTGG
CGCTGACGGCGGCCGAAACCGGCCACTTGGTGTTTGGCACGCTGCACACGAGTTCCGCGGCCAAGACCATCGACCGGGTG
GTCGACGTGTTCCCCTCGGATGAGAAGGACATGGTCCGCACCATGTTGTCCGAATCGCTGGAAGCAGTGATCTCGCAGAC
GCTGCTCAAGACGCGCGACGGCTCCGGCCGGGTCGCGGCGCACGAGATCATGATCTGCACGCCGGCCATCCGACACCTGA
TCCGCGAGAACAAGATCTCGCAGATGTACTCGATGATGCAGACCAGCAGCGGGCTGGGCATGCAGACACTGGATCAGTGC
CTGTCGGAGCTCATCAAGCGCTCGGCGATCAACTACGCCGACGCGCGCGCCATCGCCAAGAACCCGGACGCGTTCGCGAA
CTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

72.174

99.424

0.718
  pilT Acinetobacter baumannii D1279779

72.174

99.424

0.718
  pilT Acinetobacter baumannii strain A118

72.174

99.424

0.718
  pilT Pseudomonas aeruginosa PAK

71.304

99.424

0.709
  pilT Pseudomonas stutzeri DSM 10701

71.014

99.424

0.706
  pilT Acinetobacter baylyi ADP1

70.145

99.424

0.697
  pilT Neisseria gonorrhoeae MS11

68.208

99.712

0.68
  pilT Neisseria meningitidis 8013

68.208

99.712

0.68
  pilT Legionella pneumophila strain Lp02

67.536

99.424

0.671
  pilT Legionella pneumophila strain ERS1305867

67.536

99.424

0.671
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.062

97.118

0.651
  pilT Vibrio cholerae strain A1552

67.062

97.118

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.289

99.712

0.501
  pilU Acinetobacter baylyi ADP1

42.442

99.135

0.421
  pilU Pseudomonas stutzeri DSM 10701

42.687

96.542

0.412
  pilU Vibrio cholerae strain A1552

41.493

96.542

0.401