Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   HF909_RS03960 Genome accession   NZ_CP051169
Coordinates   819840..820280 (+) Length   146 a.a.
NCBI ID   WP_193035852.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain RUN2474     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 814840..825280
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HF909_RS03935 (HF909_03990) - 815403..815681 (-) 279 WP_193027652.1 hypothetical protein -
  HF909_RS03940 (HF909_03995) proC 815704..816528 (-) 825 WP_193027653.1 pyrroline-5-carboxylate reductase -
  HF909_RS03945 (HF909_04000) - 816563..817285 (-) 723 WP_193027654.1 YggS family pyridoxal phosphate-dependent enzyme -
  HF909_RS03950 (HF909_04005) pilT 817410..818453 (+) 1044 WP_193027655.1 type IV pilus twitching motility protein PilT Machinery gene
  HF909_RS03955 (HF909_04010) pilU 818506..819645 (+) 1140 WP_193027656.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HF909_RS03960 (HF909_04015) pilE 819840..820280 (+) 441 WP_193035852.1 type IV pilin protein Machinery gene
  HF909_RS03965 (HF909_04020) - 820284..820772 (+) 489 WP_016723684.1 Tfp pilus assembly protein FimT/FimU -
  HF909_RS03970 (HF909_04025) pilV 820769..821359 (+) 591 WP_193035854.1 type IV pilus modification protein PilV -
  HF909_RS03975 (HF909_04030) - 821356..822384 (+) 1029 WP_193035856.1 PilW family protein -
  HF909_RS03980 (HF909_04035) - 822388..822924 (+) 537 WP_193035858.1 PilX N-terminal domain-containing pilus assembly protein -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15260.31 Da        Isoelectric Point: 9.0659

>NTDB_id=382938 HF909_RS03960 WP_193035852.1 819840..820280(+) (pilE) [Ralstonia pseudosolanacearum strain RUN2474]
MASKLACQRARSARGFTLIELMIVAAIVAILAVLAYPSYVQYIVRSNRAATESFMQEVAAAQERFLLDNRAYASDLATLQ
YASSVPASVAANYQFSFTAVTTAPPAYTLNAKPTGSQASSDAACGTLGLSNTGSKTASGGASNCWK

Nucleotide


Download         Length: 441 bp        

>NTDB_id=382938 HF909_RS03960 WP_193035852.1 819840..820280(+) (pilE) [Ralstonia pseudosolanacearum strain RUN2474]
GTGGCAAGCAAGCTTGCATGCCAGCGCGCGCGGTCAGCGCGGGGCTTCACGCTGATCGAGCTCATGATCGTGGCGGCTAT
TGTCGCCATTCTTGCGGTTCTGGCGTATCCCTCTTACGTTCAGTACATCGTGCGCTCCAACCGCGCGGCGACGGAATCGT
TCATGCAGGAAGTGGCTGCGGCCCAGGAGCGCTTCCTGCTCGATAACCGAGCGTATGCCTCGGACCTGGCGACGCTGCAA
TATGCCAGCAGCGTTCCGGCCAGCGTCGCCGCCAACTACCAGTTCAGCTTCACTGCCGTTACGACGGCTCCGCCGGCTTA
TACGCTGAATGCAAAGCCGACGGGCAGCCAGGCCTCCAGCGACGCCGCGTGCGGCACGCTCGGTCTGTCGAATACCGGCA
GCAAGACGGCCTCCGGCGGTGCCTCCAACTGCTGGAAATAG

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(11-36)

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(37-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Acinetobacter baumannii D1279779

39.552

91.781

0.363
  comF Acinetobacter baylyi ADP1

39.259

92.466

0.363