Detailed information NTG0381499

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: G6008_RS18335
• Genome accession: NZ_CP050646
• Coordinates: 3835685..3836665 (+)
• Length: 326 a.a.
• NCBI ID: WP_001055620.1
• Uniprot ID: -
• Organism: Escherichia coli strain PapM-36-2
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3830685..3841665

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
G6008_RS18305 (G6008_18310)	yggM	3831200..3832207 (+)	1008	WP_000745217.1	DUF1202 family protein	-
G6008_RS18310 (G6008_18315)	hemW	3832362..3833498 (-)	1137	WP_000239944.1	radical SAM family heme chaperone HemW	-
G6008_RS18315 (G6008_18320)	rdgB	3833491..3834084 (-)	594	WP_001174743.1	XTP/dITP diphosphatase	-
G6008_RS18320 (G6008_18325)	yggU	3834092..3834382 (-)	291	WP_001277222.1	DUF167 family protein YggU	-
G6008_RS18325 (G6008_18330)	yggT	3834379..3834945 (-)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
G6008_RS18330 (G6008_18335)	yggS	3834963..3835667 (-)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
G6008_RS18335 (G6008_18340)	pilT	3835685..3836665 (+)	981	WP_001055620.1	type IV pilus twitching motility protein PilT	Machinery gene
G6008_RS18340 (G6008_18345)	ruvX	3836841..3837257 (-)	417	WP_000017111.1	Holliday junction resolvase RuvX	-
G6008_RS18345 (G6008_18350)	yqgE	3837257..3837820 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
G6008_RS18350 (G6008_18355)	gshB	3837929..3838879 (-)	951	WP_000593273.1	glutathione synthase	-
G6008_RS18355 (G6008_18360)	rsmE	3838892..3839623 (-)	732	WP_001222508.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
G6008_RS18360 (G6008_18365)	endA	3839703..3840410 (-)	708	WP_000286500.1	deoxyribonuclease I	-
G6008_RS18365 (G6008_18370)	yggI	3840505..3841002 (-)	498	WP_000858396.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35903.08 Da
• Isoelectric Point: 5.5860

>NTDB_id=381499 G6008_RS18335 WP_001055620.1 3835685..3836665(+) (pilT) [Escherichia coli strain PapM-36-2]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=381499 G6008_RS18335 WP_001055620.1 3835685..3836665(+) (pilT) [Escherichia coli strain PapM-36-2]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATATCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	49.541	100	0.497
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.541	100	0.497
pilT	Neisseria meningitidis 8013	48.78	100	0.491
pilT	Neisseria gonorrhoeae MS11	48.476	100	0.488
pilT	Acinetobacter baumannii strain A118	46.483	100	0.466
pilT	Acinetobacter baylyi ADP1	46.483	100	0.466
pilT	Acinetobacter baumannii D1279779	46.483	100	0.466
pilT	Acinetobacter nosocomialis M2	46.483	100	0.466
pilT	Pseudomonas stutzeri DSM 10701	46.483	100	0.466
pilT	Pseudomonas aeruginosa PAK	45.872	100	0.46
pilT	Legionella pneumophila strain ERS1305867	44.954	100	0.451
pilT	Legionella pneumophila strain Lp02	44.954	100	0.451
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.138	97.546	0.411
pilU	Vibrio cholerae strain A1552	40.694	97.239	0.396
pilU	Pseudomonas stutzeri DSM 10701	37.879	100	0.383
pilU	Acinetobacter baylyi ADP1	37.346	99.387	0.371
pilB	Legionella pneumophila strain ERS1305867	31.152	100	0.365